Biological Stability of Lactofermented Beetroot Juice During Refrigerated Storage
Beetroot juice fermented by Lactobacillus brevis 0944 and Lactobacillus paracasei 0920 bacteria was stored for 180 days at 4°C. During storage, samples were taken at 7, 14, 30, 90 and 180 days and the following characteristics were determined: betalain content, ability to limit chemical mutations induced by N-methyl, N'nitro, N- nitrosoguanidine in the Ames test, and the size of the population of Lactobacillus bacteria. After 7 days of refrigerated storage of fermented beet juice, betalain content was found to be 88% as against the initial content. For up to 30 days of fermented beet juice storage, betalain content remained at the same level. After 90 and 180 days of storage, the level of betalains falls drastically and amounts to 32% and 25%, respectively, as compared to the initial level. The ability to limit chemical mutations in the Ames test is 57% for Salmonella TA 98 and 58% for Salmonella TA 100 at the starting point. The antimutagenic activity of fermented beet juice is preserved at the same level for 30 days. After 90 and 180 days, antimutagenic activity decreases to 32% and 41% for Salmonella TA 98 and to 41% and 22% for Salmonella TA 100. The population of Lactobacillus bacteria sp. after fermentation is at a level of 9.11 Log10 cfu/mL. After 30 days of storage, the population of bacteria falls to 8.15 Log10 cfu/mL. Further storage leads to a decrease in the population of Lactobacillus sp. bacteria to 6.80 Log10 cfu/mL after 180 days. Fermented beet juice preserves its high biological activity for up to 30 days in refrigerated storage.
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Czapski J. Mikołajczyk K. Kaczmarek M. Relationship between antioxidant capacity of red beet juice and contents of its betalain pigments. Pol. J. Food Nutr. Sci. 2009 59 119-122.
Czyżowska A. Klewicka E. Libudzisz Z. The influence of lactic acid fermentation process of red beet juice on the stability of biologically active colorants. Eur. Food Res. Technol. 2006 223 110-116.
Frank T. Stintzing F. C. Carle R. Bitsch I. Quaas D. Strab G. Bitsch R. Netzel M. Urinary pharmacokinetics of betalains following consumption of red beet juice in healthy humans. Pharmacol. Res. 2005 52 290-297.
Haveland-Smith R. B. Evaluation of the genotoxicity of some natural food colours using bacterial assays. Mutat. Res. 1981 91 285-290.
Kanai C. Pomerleau J. Lock K. McKee M. Getting children to eat more fruit and vegetables: A systematic review. Prev. Med. 2006 42 85-95.
Kanner J. Harel S. Granit R. Betalains - a new class of dietary cationized antioxidants. J. Agric. Food Chem. 2001 49 5178-5185.
Kapadia G. J. Azuine M. A. Sridhar R. Okuda Y. Tsuruta A. Ichiishi E. Mukainake T. Takasaki M. Konoshima T. Nishino H. Tokuda H. Chemoprevention of DMBA-induced UV-B promoted NOR-1-induced TPA promoted skin carcinogenesis and DEN-induced phenobarbital promoted liver tumors in mice by extract of beetroot. Pharmacol. Res. 2003 47 141-148.
Karovičová J. Kohajdová Z. Lactic acid fermented vegetable juices. Horticultural Sci. (Prague) 2003 30 152-158.
Klewicka E. Fermented beetrrot juice as a factor limiting chemical mutations induced by MNNG in Salmonella typhimurium TA 98 and TA100 strains. Food Technol. Biotechnol. 2010 48 229-233.
Klewicka E. Zduńczyk Z. Juśkiewicz J. Effect of lactobacillus fermented beetroot juice on composition and activity of cecal microflora of rats. Eur. Food Res. Technol. 2009 229 153—-157.
Lankaputhra W. E. V. Shah N. P. Antimutagenic properties of probiotic bacteria and of organic acids. Mutat. Res. 1998 397 169-182.
Maron D. M. Ames B. N. Revised methods for Salmonella mutagenicity test. Mutat. Res. 1983 113 173-215.
Nilsson T. Studies into the pigments in beetroot (Beta vulgaris L. ssp. vulgaris var. rubra L.) Lantbrukshogskolans Annaler 1970 36 179-219.
Pátkai Gy. Barta J. Varsányi I. Decomposition of anticarcinogen factors of the beetroot during juice and nectar production. Cancer Lett. 1997 114 105-106.
Reid G. Beuerman D. Heinemann C. Bruce A. W. Probiotic Lactobacillus dose required to restore and maintain a normal vaginal flora. FEMS Immunol. Med. Microbiol. 2001 32 37—-41.
Report of a Joint WHO/FAO Expert Consultation. Diet nutrition and the prevention of chronic diseases. 2003 Geneva.
Stintzing F. C. Carle R. Functional properties of anthocyanins and betalains in plants food and in human nutrition. Trends Food Sci. Technol. 2004 15 19-38.
Stintzing F. C. Schieber A. Carle R. Identification of betalains from yellow beet (Beta vulgaris L.) and Cactus Pear [Opuntia ficus-indica(L) Mill.] by high-performance liquid chromatography-electrospray ionization mass spectrometry. J. Agric. Food Chem. 2002 50 2302-2307.
Stintzing F. C. Trichterborn J. Carle R. Characterization of anthocyanin - betalain mixtures for food colouring by chromatic and HPLC-DAD-MS analyses. Food Chem. 2006 94 296-309.
Strack D. Vogt T. Schliemann W. Recent advances in betalain research. Phytochemistry 2003 62 247-269.
Urala N. Lähteenmäki L. Consumers' changing attitudes towards functional foods. Food Qual. Prefer. 2007 18 1-12.
Yoon K. Y. Woodams E. E. Hang Y. D. Fermentation of beet juice beneficial lactic acid bacteria. LWT - Food Sci. Technol. 2005 38 73-75.