The effect of medium composition on the production of sophorolipids and the tensiometric properties by Starmerella bombicola MTCC 1910
Starmerella bombicola a teleomorph of Candida bombicola is capable of producing extracellular secondary metabolites known as sophorolipids. In the present work the performance of Starmerella in producing sophorolipids, with standard medium ingredients glucose, sunflower oil, yeast extract and urea was studied. The quantities of different medium ingredients were optimized to maximize the production of sophorolipids. Variation in tensiometric properties like surface tension and interfacial tension during the incubation period were also reported. The optimized mixed substrate composition was found to be 200 g/l, containing equal amounts of glucose and sunflower oil, 4 g/l of yeast extract and 0.6 g/l of urea. With the optimized substrate composition 38.6 g/l of sophorolipids was obtained. The minimum surface tension produced by the culture free cell broth was 36.2 mN/m. Increasing the temperature from 25°C to 35°C has shown adverse effects on sophorolipids production.
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Rosenberg E. & Ron E. Z. (1999). High molecular mass microbial surfactants. Appl. Microbiol. Biotechnol. 52 154-162. DOI 10.1007/s002530051502.
Mulligan C. N. (2005). Environmental applications for Biosurfactants Environ. Pollut. 133 183-198. DOI 10.1016/ j.envpol.2004.06.009.
Inoue S. & Ito S. (1982) Sophorolipids form Torulopsis bombicola: Possible relation to alkane uptake. Appl. Environ. Microbiol. 43 1278-1283.
Asmer H. J. Lang S. Wagner F. & Wray V. (1988) Microbial production structure elucidation and bioconversion of sophorose lipids. J Am Oil Chem Soc. 65 1460-1466. DOI 10.1007/BF02898308.
Tulloch A. P. Spenser J. F. T. & Gorin P. A. J. (1962). The fermentation of long chain compounds by Torulopsis magnoliae. Can. Journal of Chem. 40 1326-1338. DOI 10.1139/v62-203.
Gorin P. A. J. Spenser J. F. T. & Tulloch A. P. (1961) Hydroxy Fatty acid Glycosides of Sophorose from Torulopsis magnoliae. Can. Journal of Chem. 39 846-855. DOI 10.1139/ v61-104.
Linton J. D. (1991) Metabolite production and growth efficiency. Antonie van Leeuwenhoek. 60 293-311. DOI 10.1007/BF00430371.
Inge N. A. Bogaert V. Saerens K. Muynck C. D. Develter D. Soetaert W. & Vandamme E. J. (2007) Microbial production and application of Sophorolipids. Appl. Microbiol Biotechnol. 76 23-34. DOI 10.1007/s00253-007-0988-7.
Casas J. A. & Garcia-Ochoa F. (1999). Sophorolipid production by Candida bombicola: Medium composition and Culture methods. J. Biosci. Bioeng. 88 488-494. DOI 10.1016/ S1389-1723(00)87664-4.
Zhou Q. H. Klekner V. & Kosaric N. (1992) Production of sophorose lipids by Torulopsis bombicola from Safflower Oil and Glucose. Appl. Environ. Microbiol. 47 89-91. DOI 10.1007/BF02635883.
Surubbo L. A. Farias C. B. B. & Campos-Takaki G. M. (2007). Co Utilization of Canola Oil and Glucose on the production of a surfactant by Candida lipolytica. Curr. Microbiol. 54 68-73. DOI 10.1007/s00284-006-0412-z.
Ashby R. D. Nunez A. Solaiman D. K. Y. & Foglia T. A. (2005). Sophorolipid biosynthesis from a Biodiesel co product stream. J Am Oil Chem Soc. 82 625-630. DOI 10.1007/s11746-005-1120-3.
Rau U. Manzke C. & Wagner F. (1996). Influence of substrate supply on the production of sophorose lipids by Candida bombicola ATCC 22214. Biotechnol. Lett. 18 149-154. DOI 10.1007/BF00128670.
Felse P. A. Shah V. Chan J. Rao K. J. & Gross R. A. (2007). Sophorolipid biosynthesis by Candida bombicola from industrial fatty acid residues. Enzyme and Microb Technol. 40 316-323. DOI 10.1016/j.enzmietec.2006.04.013.
Fleurackers S. J. J. (2006). On the use of Waste frying oil in the synthesis of sophorolipids. Eur. J. Lipid. Sci. Technol. 108 5-12. DOI 10.1002/ejlt.200500237.
Kim Soo H. Kim Y. B. Lee B. S. & Kim E. K. (2005) Sophorolipid Production by Candida bombicola ATCC 22214 from a Corn Oil processing byproduct. J. Microbiol. Biotechnol. 15 55-58.
Cavalero D. A. & Cooper D. G. (2003). The effect of medium composition on the structure and physical state of Sophorolipids produced by Candida bombicola ATCC 22214. J. Biotechnol. 103 31-41. DOI 10.1016/S0168-1656(03)00067-1.