A new species of Tetradesmus (Chlorophyceae, Chlorophyta) isolated from desert soil crust habitats in southwestern North America

Abstract

A new species of Tetradesmus (Tetradesmus adustus) is described from desert soils of southwestern North America. The identification is based on phylogenetic analysis of data from nuclear (ITS2 rDNA) and plastid (rbcL, tufA) barcode markers. This newly described species represents the fifth cryptic species of arid-adapted algae in Scenedesmaceae. A re-analysis of published sequences attributed to desert Tetradesmus in the context of our newly obtained data reiterates the importance of robust phylogenetic analysis in identification of cryptic taxa, such as species of Tetradesmus.

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • An, S. S., Friedl, T. & Hegewald, E. 1999. Phylogenetic relationships of Scenedesmus and Scenedesmus-like coccoid green algae as inferred from ITS-2 rDNA sequence comparisons. Plant Biology 1: 418–428.

  • Belnap, J. & Lange, O. L. 2001. Biological soil crusts: structure, function, and management. Springer, Berlin.

  • Bold, H. C. 1949. The morphology of Chlamydomonas chlamydogama, Sp. Nov. Bulletin Torrey botanical club 76: 101–108.

  • Büdel, B., Darienko, T., Deutschewitz, K., Dojani, S., Friedl, T., Mohr, K. I., Salisch, M., Reisser, W. & Weber, B. 2009. Southern African biological soil crusts are ubiquitous and highly diverse in drylands, being restricted by rainfall frequency. Soil Microbiology 57: 229–247.

  • Cameron, R. E. 1960. Communities of soil algae occurring in the Sonoran desert in Arizona. Journal of Arizona Academy of Science 1: 85–88.

  • Cameron, R. E. 1964. Algae of Southern Arizona. II. Algal Flora (Exclusive of Bluegreen Algae). Review Algology 7: 151–177.

  • Cardon, Z. G., Peredo, E. L., Dohnalkova, A. C., Gershone, H. L. & Bezanilla, M. 2018. A model suite of green algae within the Scenedesmaceae for investigating contrasting desiccation tolerance and morphology. Journal of Cell Science 131: jcs212233

  • De Clerck, O., Guiry, M. D., Leliaert, F., Samyn, Y. & Verbruggen, H. 2013. Algal taxonomy: a road to nowhere? Journal of Phycology 49: 215–225.

  • Evans, R. D. & Johansen, J. R. 1999. Microbiotic crusts and ecosystem pocesses. Critical Reviews in Plant Sciences 18: 183–225.

  • Famá, P., Wysor, B., Kooistra, W. & Zuccarello, G. C. 2012. Molecular phylogeny of the genus Caulerpa (Caulerpales, Chlorophyta) inferred from chloroplast tufA gene. Journal of Phycology 38: 1040–1050.

  • Flechtner, V. R., Johansen, J. R. & Clark, W.H. 1998. Algal composition of microbiotic crusts from the Central Desert in Baja California, Mexico. Great Basin Naturalist 58: 295–311.

  • Fučíková, K., Lewis, P. O. & Lewis, L. A. 2014. Widespread desert affiliation of trebouxiophycean Algae (Trebouxiophyceae, Chlorophyta) including discovery of three new desert genera. Phycological Research 62: 294–305.

  • Guindon, S., Dufayard, J. F., Lefort, V., Anisimova, M., Hordijk, W. & Gascuel, O. 2010. New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Systematic Biology 59: 307–321.

  • Hall, J., Fučíková, K., Lo, C., Lewis, L. A. & Karol, K. 2010. An assessment of proposed DNA barcodes in freshwater green algae. Cryptogamie Algologie 31: 529–555.

  • Huss, V. A. R., Frank, C., Hartmann, E. C., Hirmer. M., Kloboucek, A., Seidel, B. M., Wenzeler, P. & Kessler, E. 1999. Biochemical taxonomy and molecular phylogeny of the genus Chlorella sensu lato (Chlorophyta). Journal of Phycology 35: 587–598.

  • Koetschan, C., Hackl, T., Müller, T., Wolf, M., Förster, F. & Schultz, J. 2012. ITS2 database IV: interactive taxon sampling for Internal Transcribed Spacer 2 based phylogenies. Molecular Phylogenetics and Evolution 63: 585–588.

  • Lanfear, R., Calcott, B., Ho, S. Y. & Guindon, S. 2012. PartitionFinder: combined selection of partitioning schemes and substitution models for phylogenetic analyses. Molecular Biology and Evolution 29: 1695–1701.

  • Lanfear, R., Frandsen, P. B., Wright, A. M., Senfeld, T. & Calcott, B. 2017. PartitionFinder 2: new methods for selecting partitioned models of evolution for molecular and morphological phylogenetic analyses. Molecular Biology and Evolution 34: 772–773.

  • Lange, O. L., Kidron, G. J., Büdel, B., Meyer, A., Kilian, E. & Abeliovich, A. 1992. Taxonomic composition and photosynthetic characteristics of the biological soil crusts covering sand dunes in the Western Negev Desert. Functional Ecology 56: 519–527.

  • Lewis, L. A. & Flechtner, V. R. 2004. Cryptic species of Scenedesmus (Chlorophyta) from desert soil communities of Western North America. Journal of Phycology 40: 1127–1137.

  • Lewis, L. A. & Flechtner, V. R. 2019. Tetradesmus bajacalifornicus L.A.Lewis & Flechtner, sp. nov. and Tetradesmus deserticola L.A.Lewis & Flechtner, sp. nov. (Scenedesmaceae, Chlorophyta). Notulae algarum No. 88.

  • Lewis, L. A. & Lewis, P. O. 2005. Unearthing the molecular phylodiversity of desert soil green algae (Chlorophyta). Systematic Biology 54: 936–947.

  • Lürling, M. & Van Donk, E. 1996. Zooplankton-induced unicell-colony transformation in Scenedesmus acutus and its effect on growth of herbivore Daphnia. Oecologia 108: 432–437.

  • Mikhailyuk, T., Glaser, K., Tsarenko, P., Demchenko, E. & Karsten, U. 2018. Composition of biological soil crusts from sand dunes of the Baltic Sea coast, in the context of an integrative approach to the taxonomy of microalgae and cyanobacteria. European Journal of Phycology. In press.

  • Miller, M. A., Pfeiffer, W. & Schwartz, T. 2010. Creating the CIPRES Science Gateway for inference of large phylogenetic trees. Proceedings of the Gateway Computing Environments Workshop 14 Nov. 2010, New Orleans, LA, pp. 1–8.

  • Muggia, L., Leavitt, S. & Barreno, E. 2018. The hidden diversity of lichenised Trebouxiophyceae (Chlorophyta). Phycologia 57: 503–524.

  • Ocampo-Paus, R. & Friedmann, I. 1966. Radiosphaera Negevensis Sp. N., a new chlorococcalean desert alga. American Journal of Botany 53: 663–671.

  • Rambout, A., Drummond, A. J., Xie, D., Baele, G. & Suchard, M. A. 2018. Posterior summarisation in Bayesian phylogenetics using Tracer 1.7. Systematic Biology 67: 901–904.

  • Ronquist, F. & Huelsenbeck, J. P. 2003. MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19: 1572–1574.

  • Schultz, J., Müller, T., Achtziger, M., Seibel, P. N., Dandekar, T. & Wolf, M. 2006. The Internal Transcribed Spacer 2 Database – a web server for (not only) low level phylogenetic analyses. Nucleic Acids Research 34: 704–707.

  • Schwarz, G. 1978. Estimating the dimension of a model. The Annals of Statistics 6: 461–464.

  • Sciuto, K., Lewis, L. A., Verleyen, E., Moro, I. & La Rocca, N. 2015. Chodatodesmus australis Sp. Nov. (Scenedesmaceae, Chlorophyta) from Antarctica, with the emended description of the genus Chodatodesmus, and circumscription of Flechtneria rotunda Gen. et Sp. Nov. Journal of Phycology 51: 1172–1188.

  • Sherwood, A. R., Neumann, J. M., Dittbern-Wang, M. & Conklin, K. Y. 2018. Diversity of the green algal genus Spirogyra (Conjugatophyceae) in the Hawaiian Islands. Phycologia 57: 331–344.

  • Song, G., Li, X. & Hui, R. 2017. Effect of biological soil crusts on seed germination and growth of an exotic and two native plant species in an arid ecosystem. PLoS ONE 12: 529–555.

  • Swofford, D. L. 2003. PAUP*. Phylogenetic analysis using parsimony (* and Other Methods). Version 4. Sinauer Associates, Sunderland.

  • Trainor, F. R., Wallett, S. & Grochowski, J. 1991. A useful algal growth medium. Journal of Phycology 27: 460–461.

  • Trobajo, R., Mann, D. G., Clavero, E., Evans, K. M., Vanormelingen, P. & McGregor, R. C. 2010. The use of partial cox1, rbcL and LSU rDNA aequences for phylogenetics and species identification within the Nitzschia palea species complex (Bacillariophyceae). European Journal of Phycology 45: 413–425.

  • White, J., Bruns, T. T. D., S. Lee, B. & Taylor, J. W. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR Protocols: A Guide to Methods and Applications 18: 315–322.

  • Zhu, X., Wang, J., Lu, Y., Chen, Q. & Yang, Z. 2015. Grazer-induced morphological defense in Scenedesmus obliquus is affected by competition against Microcystis aeruginosa. Scientific Reports 5: 12743.

  • Zou, S., Fei, C., Gao, Z., Bao, Y., He, M. & Wang, C. 2016. How DNA barcoding can be more effective in microalgae identification: a case of cryptic diversity revelation in Scenedesmus (Chlorophyceae). Scientific Reports 9: 36822.

  • Zuccarello, G. C. & West, J. A. 2003. Multiple cryptic species: molecular diversity and reproductive isolation in the Bostrychia radicans / B. moritziana complex (Rhodomelaceae, Rhodophyta) with focus on North American isolates. Journal of Phycology 39: 948–959.

OPEN ACCESS

Journal + Issues

Search