The stable EPR signal produced by ionizing radiation in crystalline D-mannose (C6H12O6) and separated from cranberries (Vaccinium oxycoccus) was studied. The isothermal heating of irradiated sample at 95°C for 10 minutes (melting point of D-mannose is 132°C) resulted in the modification and simplification of the EPR signal involved. The isotropic quartet has been recognized in the EPR signal of heat-treated sample. Molecular structure of the isotropic quartet identified in the complex EPR signal of D-mannose crystallite is proposed.
If the inline PDF is not rendering correctly, you can download the PDF file here.
1. European Committee for Standardization (CEN). (2003). European Standard: Foodstuffs – Detection of irradiated food containing crystalline sugar by ESR spectroscopy. EN 13708:2003. (ICS 67.050). Brussels.
2. European Committee for Standardization (CEN). (2001). European Standard: Foodstuffs – Detection of irradiated food containing cellulose by ESR spectroscopy. EN 1787:2001. (ICS 67.050). Brussels.
3. Poradnik fizykochemiczny (2nd ed.). (1974). Warszawa: Wydawnictwa Naukowo-Techniczne WNT.
5. Guzik, G. P., Stachowicz, W., & Michalik, J. (2008). Study on stable radicals produced by ionising radiation in dried fruits and related sugars by electron paramagnetic resonance spectrometry and photo-stimulated luminescence method – I. D-fructose. Nukleonika, 53(Suppl. 2), S89–S94.
6. Guzik, G. P., & Stachowicz, W. (2013). Study on radicals giving rise to multicomponent EMR spectra in dried fruits exposed to ionizing radiation II. D-Glucose. Nukleonika, 58(3), 425–428.
7. Guzik, G. P., & Stachowicz, W. (2016). Study on radiation-induced radicals giving rise to stable EPR signal suitable for the detection of irradiation in L-sorbose-containing fruits. Nukleonika, 61(4), 461–465. doi: 10.1515/nuka-2016-0075.
8. Vanhaelewyn, G., Jansen, B., Callens, F. J., & Sagstuen, E. (2004). ENDOR – Assisted study of the stable EPR spectrum of X-irradiated α-L-Sorbose single crystals: MLCFA and simulation decomposition analyses. Radiat. Res., 162, 96–104.
9. Jepson, R. G., Williams, G., & Craig, J. C. (2013). Cranberries for preventing urinary tract infections. Sao Paulo Med. J., 131(5), 363. DOI: 10.1590/1516-3180.2013.1315T1.
10. European Food Safety Authority. (2014). Scientific opinion on the substantiation of a health claim related to CranMax® and reduction of the risk of urinary tract infection by inhibiting the adhesion of certain bacteria in the urinary tract pursuant to Article 14 of Regulation (EC) No 1924/20061. EFSA Journal, 12(5), 3657.
11. Zeldes, B., & Leah, A. (2009, November 25). Eat this. Cranberries more than a thanksgiving condiment. Dining Chicago. Chicago’s Restaurant & Entertainment Guide, Inc. Food and Life Periodic of Chicago Restaurants.
12. Eck, P. (1990). The American cranberry. New Brunswick: Rutgers University Press.
13. Freeze, H. H., & Sharma, V. (2010). Metabolic manipulation of glycosylation disorders in humans and animal models. Semin. Cell Dev. Biol., 21(6), 655–662.
14. Alton, G., Hasilik, M., Niehues, R., Panneerselvam, K., Etchison, J. R., Fana, F., & Freeze, H. H. (1998). Direct utilization of mannose for mammalian glyco-protein biosynthesis. Glycobiology, 8(3), 285–295.
15. Davis, J. A., & Freeze, H. H. (2001). Studies of man-nose metabolism and effects of long-term mannose ingestion in the mouse. Biochim. Biophys. Acta, 1528(2/3), 116–126.
16. Vlahopoulos, S., Gritzapis, A. D., Perez, S. A., Cacoullos, N., Papamichail, M., & Baxevanis, C. N. (2009). Mannose addition by yeast Pichia Pastoris on recombinant HER-2 protein inhibits recognition by the monoclonal antibody herceptin. Vaccine, 27(34), 4704–4708.
17. Collins P. M. (Ed.). (2005). Dictionary of carbohydrates (2nd ed.). Boca Raton: CRC Press.
18. The President of the Office of the Registration of Curative Products, Medical Goods and Bioactive Products. (2014). The Polish pharmacopoeia. Pharmacopoea polonica. Vol. 1. Lumina the Publishing-Group.
19. European Communities. (1999). Directive 1999/2/EC of the European Parliament and of the Council of 22 February 1999 on the approximation of the laws of the Member States concerning foods and food ingredients treated with ionizing radiation. Official Journal of the European Communities, 13.3.1999, L66/16-L66/23.
20. European Communities. (1999). Directive 1999/3/EC of the European Parliament and of the Council of 22 February 1999 on the establishment of a Community list of foods and food ingredients treated with ionizing radiation. Official Journal of the European Communities, 13.3.1999, L66/24-L66/25.
21. FAO/WHO. (2003). General standard for irradiated foods. Codex Stan 106–1983. Rev.1.
22. Ayscough, P. B. (1967). Electron spin resonance in chemistry. Leeds: Department of Physical Chemistry of Leeds University.