Studies of unicellular microorganisms Saccharomyces cerevisiae by means of positron annihilation lifetime spectroscopy

Ewelina Kubicz 1 , Bożena Jasińska 3 , Bożena Zgardzińska 3 , Tomasz Bednarski 1 , Piotr Białas 1 , Eryk Czerwiński 1 , Aleksander Gajos 1 , Marek Gorgol 3 , Daria Kamińska 1 , Łukasz Kapłon 2 , Andrzej Kochanowski 4 , Grzegorz Korcyl 1 , Paweł Kowalski 5 , Tomasz Kozik 1 , Wojciech Krzemień 6 , Szymon Niedźwiecki 1 , Marek Pałka 1 , Lech Raczyński 5 , Zenon Rajfur 1 , Zbigniew Rudy 1 , Oleksandr Rundel 1 , Neha Gupta Sharma 1 , Michał Silarski 1 , Artur Słomski 1 , Adam Strzelecki 1 , Anna Wieczorek 2 , Wojciech Wiślicki 5 , Marcin Zieliński 1 , and Paweł Moskal 1
  • 1 Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, 11 S. Łojasiewicza Str., 30-348 Kraków, Poland
  • 2 Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, 11 S. Łojasiewicza Str., 30-348 Kraków, Poland and Institute of Metallurgy and Materials Science PAS, 25 W. Reymonta Str., 30-059 Kraków, Poland
  • 3 Department of Nuclear Methods, Institute of Physics, Maria Curie-Sklodowska University, 1 M. Curie-Sklodowskiej Sq., 20-031 Lublin, Poland
  • 4 Faculty of Chemistry, Department of Chemical Technology, Jagiellonian University, 3 R. Ingardena Str., 30-059 Kraków, Poland
  • 5 Świerk Computing Centre, National Centre for Nuclear Research, 7 Andrzeja Soltana Str., 05-400 Otwock/Świerk, Poland
  • 6 High Energy Physics Division, National Centre for Nuclear Research, 7 Andrzeja Soltana Str., 05-400 Otwock/Świerk, Poland

Abstract

Results of positron annihilation lifetime spectroscopy (PALS) and microscopic studies on simple microorganisms, brewing yeasts, are presented. Lifetime of ortho-positronium (o-Ps) were found to change from 2.4 to 2.9 ns (longer-lived component) for lyophilized and aqueous yeasts, respectively. Also hygroscopicity of yeasts in time was examined, allowing to check how water – the main component of the cell – affects PALS parameters, thus lifetime of o-Ps were found to change from 1.2 to 1.4 ns (shorter-lived component) for the dried yeasts. The time sufficient to hydrate the cells was found below 10 hours. In the presence of liquid water, an indication of reorganization of yeast in the molecular scale was observed. Microscopic images of the lyophilized, dried, and wet yeasts with best possible resolution were obtained using inverted microscopy (IM) and environmental scanning electron microscopy (ESEM) methods. As a result, visible changes to the surface of the cell me mbrane were observed in ESEM images.

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