Phytotoxicity of colloidal solutions of stabilized and non-stabilized nanoparticles of essential metals and their oxides

Open access


Advances in nanotechnology in various fields of human activity contribute to increase of their production, improved properties and wider implementation of nanomaterials. However, increasing use may enhance their release into the environment and can lead to affecting human health. The toxicity of colloidal solutions of metal nanoparticles (Cu, Mn) and their oxides, obtained in the absence and presence of a stabilizer, was examined and compared with the use of the standard test system of Allium cepa L.. The phytotoxicity of the experimental solutions was evaluated according to the growth response of the onion roots; the cyto- and genotoxicity were estimated due to the proliferative activity of the root meristem cells. It was established that solutions of stabilized metal nanoparticles were at given concentration toxic to Allium cepa L. according to the integral index of roots growth, however, were not cytotoxic. Difference in the phytotoxicity of stabilized and non-stabilized metal nanoparticles and their oxides depended on their phase composition and affected root growth.

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • Abdel Latef AAH Abu Alhmad MF Abdelfattah KE (2017) The possible roles of priming with ZnO nanoparticles in mitigation of salinity stress in lupine (Lupinus termis) plants. J. Plant Growth Regul. 36: 60-70.

  • Auffan M Rose J Bottero JY Lowry GV Jolive JP Wiesner MR (2009) Towards a definition of inorganic nanoparticles from an environmental health and safety perspective. Nat. Nanotechnol. 4: 634-641.

  • Barbez E Dünser K Gaidora A Lendl T Busch W (2017) Apoplastic pH regulation in A. thaliana roots. Proc. Natl. Acad. Sci. USA. 114: E4884-E4893.

  • Cunningham S Brennan-Fournet ME Ledwith D Byrnes L Joshi L (2013) Effect of nanoparticle stabilization and physicochemical properties on exposure outcome: acute toxicity of silver nanoparticle preparations in zebrafish (Danio rerio). Environ. Sci. Technol. 47: 3883-3892.

  • Dimkpa CO Calder A Britt DW McLean JE Anderson AJ (2011) Responses of a soil bacterium Pseudomonas chlororaphis O6 to commercial metal oxide nanoparticles compared with responses to metal ions. Environ. Pollut. 159: 1749-1756.

  • Ditta A Arshad M (2016) Applications and perspectives of using nanomaterials for sustainable plant nutrition. Nanotechnol. Rev. 5: 209-229.

  • Fiskesjo G (1997) Allium-test for screening chemicals; evaluation of cytologic parameters. In Wang W Gorsuch JW Hughes JS (Eds.) Plants for environmental studies Boca Raton CRC Lewis Publishers New York p. 308.

  • Fiskesjo G (1985) The Allium-test as a standard in environmental monitoring. Hereditas. 10: 99-112.

  • Fu PP Xia Q Hwang H-M Ray PC Yu H (2014) Mechanisms of nanotoxicity: Generation of reactive oxygen species. J. Food Drug Anal. 22: 64-75.

  • Gottschalk F Sun T Nowak B (2013) Environmental concentrations of engineered nanomaterials: review of modeling and analytical studies. Environ. Pollut. 181: 287-300.

  • Griffitt RJ Luo J Gao J Bonzongo J Barber DS (2008) Effects of particle composition and species on toxicity of metallic nanomaterials in aquatic organisms. Environ. Toxicol. Chem. 27: 1972-1978.

  • Horie M Fujita K Kato H Endoh S Nishio K Komaba LK Nakamura A Miyauchi A Kinugasa H Hagihara Y Niki E Yoshida Y Iwahashi H (2012) Association of the physical and chemical properties and the cytotoxicity of metal oxide nanoparticles: metal ion release adsorption ability and specific surface area. Metallomics. 4: 350-360.

  • Konate A He X Zhang Z Ma Y Zhang P Alugongo GM Rui Y (2017) Magnetic (Fe3O4) nanoparticles reduce heavy metals uptake and mitigate their toxicity in wheat seedling. Sustainability 9: 790.

  • Konotop YeO Kovalenko MS Ulynets VZ Meleshko AO Batsmanova LM Taran NYu (2014) Phytotoxicity of colloidal solutions of metal-containing nanoparticles. Cytol. Genet. 48: 99-102.

  • Kumari M Mukherjee A Chandrasekaran N (2009) Genotoxicity of silver nanoparticles in Allium cepa. Sci. Total Environ. 407: 5243-5246.

  • Kumari M Khan SS Pakrashi S Mukherjee A Chandrasekaran N (2011) Cytogenetic and genotoxic effects of zinc oxide nanoparticles on root cells of Allium cepa. J. Hazard. Mater. 190: 613-621.

  • Lee S Kim S Lee I (2012) Effects of soil-plant interactive system on response to exposure to ZnO nanoparticles. J. Microbiol. Biotechnol. 22: 1264-1270.

  • Lin SY Tsai YT Chen C-C Lin CM Chen C-H (2004) Two-step functionalization of neutral and positively charged thiols onto citrate-stabilized Au nanoparticles. J. Phys. Chem. B. 108: 2134-2139.

  • Livanos P Apostolakos P Galatis B (2012) Plant cell division: ROS homeostasis is required. Plant Signal. Behav. 7: 771-778.

  • Liu RQ Zhang HY Lal R (2016) Effects of stabilized nanoparticles of copper zinc manganese and iron oxides in low concentrations on lettuce (Lactuca sativa) seed germination: nanotoxicants or nanonutrients? Water Air Soil Pollut. 227: 1-14.

  • Lopatko KG UA Patent 38459 2009.

  • Ma H Williams PL Diamond SA (2013) Ecotoxicity of manufactured ZnO nanoparticles - a review. Environ. Pollut. 172: 76-85.

  • Mirzajani F Askari H Hamzelou S Farzaneh M Ghassempour A (2013) Effect of silver nanoparticles on Oryza sativa L. and its rhizosphere bacteria. Ecotox. Environ. Safe. 88: 48-54.

  • Nagaonkar D Shende S Rai M (2015) Biosynthesis of copper nanoparticles and its effect on actively dividing cells of mitosis in Allium cepa. Biotechnol. Prog. 31: 557-565.

  • Navarro E Baun A Behra R Hartmann NB Filser J Miao AJ Quigg A Santschi PH Sigg L (2008) Environmental behavior and ecotoxicity of engineered nanoparticles to algae plants and fungi. Ecotoxicology 17: 372-386.

  • Olkhovych O Svietlova N Konotop Y Karaushu O Hrechishkina S (2016) Removal of metal nanoparticles colloidal solutions by water plants. Nanoscale Res. Lett. 11: 518.

  • Pakrashi S Jain N Dalai S Jayakumar J Chandrasekaran PT Raichur AM (2014) In vivo genotoxicity assessment of titanium dioxide nanoparticles by Allium cepa root tip assay at high exposure concentrations. PLoS One. 9: 877-889.

  • Pradhan S Patra P Das S Chandra S Mitra S Dey KK Akbar S Palit P Goswami A (2013) Photochemical modulation of biosafe manganese nanoparticles on Vigna radiata: a detailed molecular biochemical and biophysical study. Environ. Sci. Technol. 47: 13122-13131.

  • Rayle DL Cleland RE (1992) The acid growth theory of auxin-induced cell elongation is alive and well. Plant Physiol. 99: 1271-1274.

  • Rui MM Ma CX Hao Y Guo J Rui YK Tang XL Zhao Q Fan X Zhang ZT Hou TQ Zhu SY (2016) Iron oxide nanoparticles as a potential iron fertilizer for peanut (Arachis hypogaea). Front. Plant Sci. 7: 815.

  • Ruttkay-Nedecky B Krystofova O Nejdl L Adam V (2017) Nanoparticles based on essential metals and their phytotoxicity. J. Nanobiotechnology 15: 33.

  • Saxena R Tomar RS Kumar M (2016) Exploring nanobiotechnology to mitigate abiotic stress in crop plants. J. Pharm. Sci. Res. 8: 974-980.

  • Scrinis G Lyons K (2007) The emerging nano-corporate paradigm: nanotechnology and the transformation of nature food and agri-food systems. Int. J. Sociol. Food Agric. 15: 22–44.

  • Sharma VK Siskova KM Zboril R Gardea-Torresdey JL (2014) Organic-coated silver nanoparticles in biological and environmental conditions: fate stability and toxicity. Adv. Colloid Interface Sci. 204: 15-34.

  • Sperling RA Parak WJ (2010) Surface modification functionalization and bioconjugation of colloidal inorganic nanoparticles. Phil. Trans. R. Soc. A. 368: 1333-1383.

  • Tang Y He R Zhao J Nie G Xu L Xing B (2016) Oxidative stress-induced toxicity of CuO nanoparticles and related toxicogenomic responses in Arabidopsis thaliana. Environ. Pollut. 212: 605-614.

  • Taran N Batsmanova L Kovalenko M Okanenko A (2016) Impact of metal nanoform colloidal solution on the adaptive potential of plants. Nanoscale Res. Lett. 11: 89.

  • Teplicky T Chorvat D Michalka M Marcek Chorvatova A (2018) Preparation of metal nanoparticles by femtosecond laser ablation. Nova Biotechnol. Chim. 17: 38-47.

  • Trujillo-Reyes J Majumdar S Botez CE Peralta-Videa JR Gardea-Torresdey JL (2014) Exposure studies of core-shell Fe/Fe3O4and Cu/CuO NPs to lettuce (Lactuca sativa) plants: are they a potential physiological and nutritional hazard? J. Hazard. Mater. 267: 255-263.

  • Van NL Ma C Shang J Rui Y Liu S Xing B (2016) Effects of CuO nanoparticles on insecticidal activity and phytotoxicity in conventional and transgenic cotton. Chemosphere. 144: 661-670.

  • Wilkins DA (1978) The measurement of tolerance to edaphic factors by means of root length. New Phytol. 80: 623-633.

Journal information
Impact Factor

CiteScore 2018: 0.68

SCImago Journal Rank (SJR) 2018: 0.173
Source Normalized Impact per Paper (SNIP) 2018: 0.288

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 183 183 30
PDF Downloads 156 156 27