Characterization and assessment of biosurfactant producing indigenous hydrocarbonoclastic bacteria: potential application in bioremediation

Open access

Abstract

Petroleum and hydrocarbons contamination can be remediated by physical, chemical or biological methods. Among these, in situ bioremediation is considered to be environmentally friendly because it restores the soil structure, requires less energy input and involves the notable removal after degradation of biosurfactant. The present study involves the characterization and assessment of biosurfactant producing indigenous hydrocarbonoclastic bacteria and their potential application in bioremediation processes. Three bacterial strains were isolated from various crude oil contaminated environments and characterized using standard identification techniques. The results clearly demonstrate the capability of utilizing hydrocarbon and biosurfactant produced by the bacterial strains. 16S rDNA sequencing followed by BLAST analysis revealed their similarity to Pseudomonas aeruginosa. The physico-chemical characterization of the biosurfactants revealed significant surface properties with stability at extreme temperature conditions (up to 121˚C), pH (5 - 8) and salinity (up to 4 %). Further, the mass spectrometry confirmed predominance of di-rhamnolipids in biosurfactant mixtures. The biosurfactants were found to be efficient in the removal of crude oil from the contaminated sand suggesting its applicability in bioremediation technology. Further, improved discharge of crude oil at elevated temperatures also confirms their thermo-stability which, could be exploited in microbial enhanced oil recovery processes. Thus, the applications of biosurfactants produced by the indigenous hydrocarbonoclastic strains appeared to be advantageous for bioremediation of petroleum-contaminated environments.

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • Aparna A Srinikethan G Smitha H (2012) Production and Characterization of biosurfactant produced by a novel Pseudomonas sp. 2B. Colloids Surf. B Biointerfaces 95: 2-29.

  • Banat IM (1993) The isolation of a thermophilic biosurfactant producing Bacillus sp. Biotechnol. Lett. 15: 591-594.

  • Benincasa M Accorsini FR (2008) Pseudomonas aeruginosa LBI production as an integrated process using the wastes from sunflower-oil refining as a substrate. Bioresour. Technol. 99: 3843-3849.

  • Bharali P Konwar BK (2011) Production and physicochemical characterization of a biosurfactant produced by Pseudomonas aeruginosa OBP1 isolated from petroleum sludge. Appl. Biochem. Biotechnol. 164(8): 1444-1460.

  • Bharali P Singh SP Dutta N Gogoi S Bora LC Debnath P Konwar BK (2014) Biodiesel derived waste glycerol as an economic substrate for biosurfactant production using indigenous Pseudomonas aeruginosa. RSC Adv. 4: 38698-38706.

  • Bodour AA Maier RM (1998) Application of a modified drop collapse technique for surfactant quantification and screening of biosurfactant-producing microorganisms. J. Microbiol. Methods 32: 273-280.

  • Bordoloi NK Konwar BK (2007) Microbial surfactantenhanced mineral oil recovery under laboratory conditions. Colloids Surf. B Biointerfaces 63: 73-82.

  • Chandrasekaran EV BeMiller JN (1980) Constituents analysis of glycosaminoglycans. In Whistler RL (Ed.) Methods in carbohydrate chemistry Academic Press New York USA 349 p.

  • Chen J Wu Q Hua Y Chen J Zhang H Wang H (2017) Potential applications of biosurfactant rhamnolipids in agriculture and biomedicine. Appl. Microbiol. Biotechnol. 101: 8309-8319.

  • De S Malik S Ghosh A Saha R Saha B (2015) A review on natural surfactants. RSC Adv. 5: 65757-65767.

  • García-Reyes S Yáñez-Ocampo G Wong-Villarreal A Rajaretinam RK Thavasimuthu C Patiño R Ortiz- Hernández ML (2017) Partial characterization of a biosurfactant extracted from Pseudomonas sp. B0406 that enhances the solubility of pesticides. Environ. Technol. 22: 1-10.

  • Haba E Pinazo A Jauregui O Espuny MJ Infante MR Manresa A (2003) Physicochemical characterization and antimicrobial properties of rhamnolipids produced by Pseudomonas aeruginosa 47T2 NCBIM 40044. Biotechnol. Bioeng. 81: 316-322.

  • Hajibagheri F Lashkarbolooki M Ayatollahi S Hashemi A (2017) The synergic effects of anionic and cationic chemical surfactants and bacterial solution on wettability alteration of carbonate rock: An experimental investigation. Colloids Surf. A 513: 422-429.

  • Irorere VU Tripathi L Marchant R McClean S Banat IM (2017) Microbial rhamnolipid production: a critical reevaluation of published data and suggested future publication criteria. Appl. Microbiol. Biotechnol. 101: 3941-3951.

  • Johnson M Boese-Marrazzo D (1980) Production and properties of heat stable extracellular hemolysin from Pseudomonas aeruginosa. Infect. Immun. 29: 1028-1033.

  • Kumar G Kumar R Sharma A (2015) Characterization of biosurfactants from indigenous soil bacteria recovered from oil contaminated sites. J. Environ. Bio. 36: 1101-1104.

  • Kumar S Tamura K Nei M (1994) MEGA: molecular evolutionary genetics analysis software for microcomputers. Comput. Appl. Biosci. 10: 189-191.

  • Kuppusamy S Thavamani P Venkateswarlu K Lee YB Naidu R Megharaj M (2017) Remediation approaches for polycyclic aromatic hydrocarbons (PAHs) contaminated soils: Technological constraints emerging trends and future directions. Chemosphere 168: 944-968.

  • Mulligan CN Gibbs BF (2004) Types production and applications of biosurfactants. Proc. Nat. Sci. Acad. 70: 31-55.

  • Parthipan P Elumalai P Sathishkumar K Sabarinathan D Murugan K Benelli G Rajasekar A (2017) Biosurfactant and enzyme mediated crude oil degradation by Pseudomonas stutzeri NA3 and Acinetobacter baumannii MN3. 3 Biotech 7: 278.

  • Pristas P Stramova Z Kvasnova S Judova J Perhacova Z Vidova B Sramkova Z Godany A (2015) Non-ferrous metal industry waste disposal sites as a source of polyextremotolerant bacteria. Nova Biotechnol. Chim. 14: 62-68.

  • Radzuan MN Banat IM Winterburn J (2017) Production and characterization of rhamnolipid using palm oil agricultural refinery waste. Bioresour. Technol. 225: 99-105.

  • Rahman MF Rusnam M Gusmanizar N Masdor NA Lee CH Shukor MS Roslan MAH Shukor MY (2016)

  • Molybdate-reducing and SDS-degrading Enterobacter sp. Strain Neni-13. Nova Biotechnol. Chim. 15: 166-181. Saikia RR Deka S Deka M Banat IM (2012) Isolation of biosurfactant-producing Pseudomonas aeruginosa RS29 from oil-contaminated soil and evaluation of different nitrogen sources in biosurfactant production. Ann. Microbiol. 62: 753-763.

  • Siegmund I Wagner F (1991) New method for detecting rhamnolipids excreted by Pseudomonas species during growth on mineral agar. Biotechnol. Technol. 5: 265-268.

  • Silva MA Silva AF Rufino RD Luna JM Santos VA Sarubbo LA (2017) Production of biosurfactants by Pseudomonas species for application in the petroleum industry. Water Environ. Res. 89: 117-126.

  • Suthar H Hingurao K Desai A Nerurkar A (2008) Evaluation of bioemulsifier mediated microbial enhanced oil recovery using sand pack column. J. Microbiol. Methods 75: 225-230.

  • Wittgens A Kovacic F Müller MM Gerlitzki M Santiago- Schübel B Hofmann D Tiso T Blank LM Henkel M Hausmann R Syldatk C Wilhelm S Rosenau F (2017) Novel insights into biosynthesis and uptake of rhamnolipids and their precursors. Appl. Microbiol. Biotechnol. 101: 2865-2878.

  • Wyrwas B Chrzanowski Ł Ławniczak Ł Szulc A Cyplik P Białas W Szymański A Hołderna-Odachowska A (2012) Utilization of Triton X-100 and polyethylene glycols during surfactant-mediated biodegradation of diesel fuel. J. Hazard. Mater. 197: 97-103.

  • Xia WJ Dong HP Yu L Yu DF (2011) Comparative study of biosurfactant produced by microorganisms isolated from formation water of petroleum reservoir. Colloids Surf. A Biointerfaces 392: 124-130.

  • Yonebayashi H Yoshida S Ono K Enomoto H (2000) Screening of microorganisms for microbial enhanced oil recovery process. Sekiyu Gakkaishi 43: 59-69.

  • Zhang Y Zhao Q Jiang J Wang K Wei L Ding J Yu H (2017) Acceleration of organic removal and electricity generation from dewatered oily sludge in a bioelectrochemical system by rhamnolipid addition. Bioresour. Technol. 243: 820-827.

  • Zaman AU (2010) Comparative study of municipal solid waste treatment technologies using life cycle assessment method. Int. J. Environ. Sci. 7: 225-235.

Search
Journal information
Impact Factor


CiteScore 2018: 0.68

SCImago Journal Rank (SJR) 2018: 0.173
Source Normalized Impact per Paper (SNIP) 2018: 0.288


Metrics
All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 1060 1060 27
PDF Downloads 320 320 17