Zinc bioaccumulation by microbial consortium isolated from nickel smelter sludge disposal site

Open access


Heavy metal pollution is one of the most important environmental issues of today. Bioremediation by microorganisms is one of technologies extensively used for pollution treatment. In this study, we investigated the heavy metal resistance and zinc bioaccumulation by microbial consortium isolated from nickel sludge disposal site near Sereď (Slovakia). The composition of consortium was analyzed based on MALDI-TOF MS of cultivable bacteria and we have shown that the consortium was dominated by bacteria of genus Arthrobacter. While consortium showed very good growth in the zinc presence, it was able to remove only 15 % of zinc from liquid media. Selected members of consortia have shown lower growth rates in the zinc presence but selected isolates have shown much higher bioaccumulation abilities compared to whole consortium (up to 90 % of zinc removal for NH1 strain). Bioremediation is frequently accelerated through injection of native microbiota into a contaminated area. Based on data obtained in this study, we can conclude that careful selection of native microbiota could lead to the identification of bacteria with increased bioaccumulation abilities.

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • Ahmed MJ Alam M (2003) A rapid spectrophotometric method for the determination of mercury in environmental biological soil and plant samples using diphenylthiocarbazone. Spectroscopy 17: 45-52.

  • Alvarez A Saez JM Costa JSD Colin VL Fuentes MS Cuozzo SA Amoroso MJ (2017) Actinobacteria: Current research and perspectives for bioremediation of pesticides and heavy metals. Chemosphere 166: 41-62.

  • Boriová K Urík M Matus P (2015) Biosorption bioaccumulation biovolatilization of potentially toxic elements by microorganisms. Chem. Listy 1092: 109-112.

  • Carpio IEM Franco DC Sato MIZ Sakata S Pellizari VH Ferreira Filho SS Rodrigues DF (2016) Biostimulation of metal-resistant microbial consortium to remove zinc from contaminated environments. Sci. Total Environ. 550: 670-675.

  • Dixit R Malaviya D Pandiyan K Singh UB Sahu A Shukla R Paul D (2015) Bioremediation of heavy metals from soil and aquatic environment: an overview of principles and criteria of fundamental processes. Sustainability 7: 2189-2212.

  • Gikas P (2008) Single and combined effects of nickel (Ni (II)) and cobalt (Co (II)) ions on activated sludge and on other aerobic microorganisms: a review.J. Hazard. Mater. 159: 187-203.

  • Gryta A Frąc M Oszust K (2014) The application of the Biolog EcoPlate approach in ecotoxicological evaluation of dairy sewage sludge. Appl. Biochem. Biotechnol. 174: 1434-1443.

  • Guo H Luo S Chen L Xiao X Xi Q Wei W He Y (2010) Bioremediation of heavy metals by growing hyperaccumulaor endophytic bacterium Bacillus sp. L14. Bioresour. Technol. 101: 8599-8605.

  • Henne KL Nakatsu CH Thompson DK Konopka AE (2009) High-level chromate resistance in Arthrobacter sp. strain FB24 requires previously uncharacterized accessory genes. BMC Microbiol. 9: 1-14.

  • Hussein H Farag S Kandil K Moawad H (2005) Tolerance and uptake of heavy metals by Pseudomonas. Process Biochem. 40: 955-961.

  • Insam H (1997) A new set of substrates proposed for community characterization in environmental samples. Springer Verlag Heidelberg Germany 261 p.

  • Kopcakova A Stramova Z Kvasnova S Godany A Perhacova Z Pristas P (2014) Need for database extension for reliable identification of bacteria from extreme environments using MALDI TOF mass spectrometry. Chem. Pap. 68: 1435-1442.

  • Ledin M (2000) Accumulation of metals by microorganisms - processes and importance for soil systems. Earth-Sci. Rev. 51: 1-31.

  • Ludwig W Euzéby J Schumann P Busse HJ Trujillo ME Kämpfer P Whitman WB (2012) Road map of the phylum Actinobacteria. In Bergey’s manual® of systematic bacteriology. Springer Verlag New York USA 1-28.

  • Malik A (2004) Metal bioremediation through growing cells. Environ. Int. 30: 261-278.

  • Mayor DJ Gray NB Elver-Evans J Midwood AJ Thornton B (2013) Metal-macrofauna interactions determine microbial community structure and function in copper contaminated sediments. PLoS One 8: 64-94.

  • Michaeli E Boltižiar M Solár V Ivanová M (2012) The landfill of industrial waste - lúženec near the former Nickel Smelter at Sereď Town as an example of environmental load. Životné prostredie 46: 63-68 (in Slovak).

  • Otth L Solis G Wilson M Fernandez H (2005) Susceptibility of Arcobacter butzleri to heavy metals Brazil J. Microbiol. 36: 286-288.

  • Pristas P Stramova Z Kvasnova S Judova J Perhacova Z Vidova B Godany A (2015) Non-ferrous metal industry waste disposal sites as a aource of poly-extremotolerant bacteria. Nova Biotechnol. Chim. 14: 62-68.

  • Remenár M Karelová E Harichová J Zámocký M Krčová K Ferianc P (2014) Actinobacteria occurrence and their metabolic characteristics in the nickel-contaminated soil sample. Biologia 69: 1453-1463.

  • Singh PK Singh AL Kumar A Singh MP (2012) Mixed bacterial consortium as an emerging tool to remove hazardous trace metals from coal. Fuel 102: 227-230.

  • Schmidt T Schlegel HG (1989) Nickel and cobalt resistance of various bacteria isolated from soil and highly polluted domestic and industrial wastes. FEMS Microbiol. Ecol. 5: 315-328.

  • Valentine NB Bolton HJR Kingsley MT Drake GR Balkwill DL Plymate AE (1996) Biosorption of cadmium cobalt nickel and strontium by a Bacillus simplex strain isolated from the vadose zone. J. lndust. Microbiol. 16: 189-l96.

  • Wu LF Navarro C De Pina K Quenard M Mandrand MA (1994) Antagonistic effect of nickel on the fermantative growth of Escherichia coli K-12 and comparison of nickel and cobalt toxicity on the aerobic and anaerobic growth. Environ. Health Perspect. 102: 297-300.

Journal information
Impact Factor

CiteScore 2018: 0.68

SCImago Journal Rank (SJR) 2018: 0.173
Source Normalized Impact per Paper (SNIP) 2018: 0.288

Cited By
All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 726 524 9
PDF Downloads 195 136 3