Genetic diversity of Acinetobacter spp. adapted to heavy metal polluted environments

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Abstract

Multiple metallotolerant bacterial strains were isolated from soil and drainage water samples collected from three industrially heavy metals polluted areas in Slovakia. Obtained bacterial isolates were identified using MALDI-TOF mass spectrometry and bacterial isolates that belonged to the Acinetobacter genus were subjected for the further study. A. calcoaceticus was found to be prevalent species among analyzed Acinetobacter spp. strains, followed by A. lwoffii and A. johnsonii. A. calcoaceticus strains exhibited higher minimum inhibitory concentration to Mn, Zn, and Cu cations compared to A. lwoffii and A. johnsonii. On the other hand minimum inhibitory concentration to Co and Ni were identical in all Acinetobacter spp. isolates. Genetic analyses demonstrated multiple plasmids presence in A. lwoffii and A. johnsonii but not in A. calcoaceticus. Using ERIC-PCR the presence of two different genotypes of A. calcoaceticus was detected in heavy metal polluted environments in Slovakia.

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  • Abboud MM Khleifat KM Batarseh M Tarawneh KA Al- Mustafa A Al-Madadhah M (2017) Different optimization conditions required for enhancing the biodegradation of linear alkylbenzosulfonate and sodium dodecyl sulfate surfactants by novel consortium of Acinetobacter calcoaceticus and Pantoea agglomerans. Enzyme Microb. Technol. 41: 432-439.

  • Abdel-El-Haleem D (2003) Acinetobacter: environmental and biotechnological applications. Afr. J. Biotechnol. 2: 71-74.

  • Dougrahi HJ Ndakidemi PA Human IS Benade S (2011) The ecology biology and pathogenesis of Acinetobacter spp.: an overview. Microbes Environ. 26: 101-112.

  • El-Sayed MH (2016) Multiple heavy metal and antibiotic resistance of Acinetobacter baumanii strain HAF-13 isolated from industrial effluents. Am. J. Microbiol. Res. 4: 26-36.

  • Ferreira L Sánchez-Juanes F García-Fraile P Rivas R Mateos PF Martínez-Molina E González-Buitrago JM Velázquez E (2011) MALDI-TOF mass spectrometry is a fast and reliable platform for identification and ecological studies of species from family Rhizobiaceae. PloS ONE 6: e20223.

  • Gaidhani S Singh R Singh D Patel U Shevade K Yeshvekar R Chopade BA (2013) Biofilm disruption activity of silver nanoparticles synthesized by Acinetobacter calcoaceticus PUCM 1005. Mater. Lett. 108: 324-327.

  • Gaidhani SV Yeshvekar RK Shedbalkar UU Bellare JH Chopade BA (2014) Bio-reduction of hexachloroplatinic acid to platinum nanoparticles employing Acinetobacter calcoaceticus. Process Biochem. 49: 2313-2319.

  • Ghodake G Jadhav S Dawkar V Govindar S (2009) Biodegradation of diazo dye Direct brown MR by Acinetobacter calcoaceticus NCIM 2890. Int. Biodeter. Biodegr. 63: 433-439.

  • Li A-D Li L-G Zhang T (2015) Exploring antibiotic resistance genes and metal resistance genes in plasmid metagenomes from wastewater treatment plants. Front. Microbiol. 6: 1025.

  • Marín M Pedregosa A Ríos S Ortiz L Laborda F (1995) Biodegradation of diesel and heating oil by Acinetobacter calcoaceticus MM5: its possible applications on bioremediation. Int. Biodeter. Biodegr. 35: 269-285.

  • Mindlin S Petrenko A Kurakov A Beletsky A Mardanov A Petrova M (2016) Resistance of permafrost and modern Acinetobacter lwoffii strains to heavy metals and arsenic revealed by genome analysis. BioMed Res. Int. Article ID 3970831.

  • Nies DH (1999) Microbial heavy-metal resistance. Appl. Microbiol. Biotechnol. 51: 730-750.

  • Pailan S Sengupta K Ganguly U Saha P (2016) Evidence of biodegradation of chlorpyrifos by a newly isolated heavy metal-tolerant bacterium Acinetobacter sp. strain MemCl4. Environ. Earth Sci. 75: 1019.

  • Raja CE Elvam GS Omine K (2009) Isolation identification and characterization of heavy metal resistent bacteria from sewage. In: International Joint Symposium on Geodisaster Prevention and Geoenvironment in Asia JS-Fukuoka.

  • Rajbanshi A (2008) Study on heavy metal resistant bacteria in Guheswori sewage treatment plant. Our Nature 6: 52-57.

  • Peng SM Liao TL Lin AC Huang TW Lauderdale TL Chen YT (2017) Sequencing and analysis of an oxacillinase-encoding plasmid from Acinetobacter spp. [online; cit. 2017-02-27 www.ncbi.nlm.nih.gov/nuccore/NC_025117.1].

  • Pristas P Stramova Z Kvasnova S Judova J Perhacova Z Vidova B Sramkova Z Godany A (2015) Non-ferrous metal industry waste disposal sites as s source of polyextremotolerat bacteria. Nova Biotechnol. Chim. 14: 62-68.

  • Versalovic J Koeuth T Lupski JR (1991) Distribution f repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes. Nucleic Acids Res. 19: 6823-6831.

  • Yavankar SP Pardesi KR Chopade BA (2007) Species distribution and physiological characterization of Acinetobacter genospecies from healthy human skin of tribal population in India. Indian J. Med. Microbiol. 25: 336-345.

  • Zong Z (2017) The complete genome of Acinetobacter johnsonii strain XBB1 carrying blaNDM-1 and blaOXA-58 from hospital sewage. [online cit. 2017-02- 27 www.ncbi.nlm.nih.gov/nuccore/NZ_CP010351.1].

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