Molybdate-reducing and SDS-degrading Enterobacter sp. Strain Neni-13

M.F. Rahman 1 , M. Rusnam 2 , N. Gusmanizar 1 , 3 , N.A. Masdor 4 , C.H. Lee 1 , M.S. Shukor 5 , M.A.H. Roslan 1  und M.Y. Shukor 1
  • 1 Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, UPM 43400, Selangor, Malaysia
  • 2 Department of Agricultural Engineering, Faculty of Agricultural Technology, Andalas University, Padang, 25163, Indonesia
  • 3 Department of Animal Nutrition, Faculty of Animal Science, Andalas University, Padang, 25163, Indonesia
  • 4 Biotechnology Research Centre, MARDI, P. O. Box 12301, Kuala Lumpur, 50774, Malaysia
  • 5 Snoc International Sdn Bhd, Lot 343, Jalan 7/16 Kawasan Perindustrian Nilai 7, Inland Port, Negeri Sembilan, 71800, Malaysia


Toxicants removal through microorganism’s action is intensely being sought due to economic reasons. The aim of this paper is to isolate a bacterium that is able to reduce molybdenum blue and at the same time can grow on the detergent Sodium Dodecyl Sulfate (SDS). Biochemical analysis resulted in a tentative identification of the bacterium as Enterobacter sp. strain Neni-13. Growth on SDS showed a 100 % removal at 800 mg/L SDS within 12 days. The removal of SDS from media was confirmed through Methylene Blue Active Substances Assay. Molybdenum reduction using sodium molybdate as a substrate was characterized using a microplate assay. The optimum pH and temperature for molybdenum reduction was between 6.0 and 6.5, and at 37 °C, respectively. Glucose was the best electron donor for molybdate reduction. Phosphate and molybdate concentrations of between 2.5 and 5.0 mM and at 15 mM, were optimal for molybdate reduction, respectively. Molybdate reduction was inhibited by the heavy metals mercury, silver, copper and chromium at 2 ppm. The ability of this bacterium to detoxify molybdate and degrade the SDS makes this bacterium an important tool for bioremediation of toxicants in soil.

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