The Activity of Cell-Wall Modifying β-1,3-Glucanases in Soybean Grown in Presence of Heavy Metals

Open access


Cell walls represent the first barrier that can prevent the entrance of toxic heavy metals into plants. The composition and the flexibility of the cell wall are regulated by different enzymes. The ß-1,3-glucanases control the degradation of the polysaccharide callose as a flexible regulation mechanism of cell wall permeability and/or its ability to bind metals under stress conditions. The profile and activity of ß-1,3-glucanases in the presence of heavy metals, however, has rarely been studied. Here we studied these enzymes in four soybean varieties (Glycine max) grown in the presence of cadmium ions. These analyses revealed three acidic and one basic enzyme isoforms in each soybean variety, but only two of the acidic isoforms in the variety Moravians were substantially responsive to the presence of Cd2+. Since the responses of certain glucanases were detected mainly in the varieties sensitive to metal and accumulating high amounts of metals, we assume their role in the defense rather than strategic metal sequestration.

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • CORRALES I. POSCHENRIEDER CH. BARCELO J.: Boron-induced amelioration of aluminium toxicity in a monocot and a dicot species. J. Plant. Physiol. 165 2008 504-513.

  • DOBROVICZKÁ T. PIRŠELOVÁ B. MÉSZÁROS P. BLEHOVÁ A. LIBANTOVÁ J. MORAVČÍKOVÁ J. MATUŠÍKOVÁ I.: Effects of cadmium and arsenic ions on content of photosynthetic pigments in the leaves of Glycine max (L.) Merrill. Pak. J. Bot. 45 2013 105-110.

  • CHAOUI A. JARRAR B. EL FERJANI E.: Effects of cadmium and copper on peroxidase NADH oxidase and IAA oxidase activities in cell wall soluble and microsomal membrane fractions of pea roots. J. Plant. Physiol. 161 2004 1225-1234.

  • MÉSZÁROS P. RYBANSKÝ L. HAUPTVOGEL P. KUNA R. LIBANTOVÁ J. MORAVČÍKOVÁ J. PIRŠELOVÁ B. TIRPÁKOVÁ A. MATUŠÍKOVÁ I.: Cultivar-specific kinetics of chitinase induction in soybean roots during exposure to arsenic. Mol. Biol. Rep. 40 2013 2127-2138.

  • METWALLY A. SAFRONOVA V.I. BELIMOV A.A. DIETZ K.J.: Genotypic variation of the response to cadmium toxicity in Pisum sativum. J. Exp. Bot. 56 2005 167-178.

  • PIRŠELOVÁ B. MISTRÍKOVÁ V. LIBANTOVÁ J. MORAVČÍKOVÁ J. MATUŠÍKOVÁ I.: Study on metal-triggered callose deposition in roots of maize and soybean. Biologia 67 2012 698-705.

  • PIRŠELOVÁ B. MATUŠÍKOVÁ I.: Callose: the plant cell wall polysaccharide with multiple biological functions. Acta Physiol. Plant. 35 2013 635-644.

  • PIRŠELOVÁ B. KUNA R. LIBANTOVÁ J. MORAVČÍKOVÁ J. MATUŠÍKOVÁ I.: Biochemical and physiological comparison of heavy metal-triggered defense responses in the monocot maize and dicot soybean roots. Mol. Biol. Rep. 38 2011 3437-3446.

  • POPPER Z. MICHEL G. HERVÉ C. DOMOZYCH D.S. WILLATS W.G. TUOHY M.G. KLOAREG B. STENGEL D.B.: Evolution and diversity of plant cell walls: from algae to flowering plants. Annu. Rev. Plant Biol. 62 2011 567-590.

  • SOCHA P. BERNSTEIN N. RYBANSKÝ Ľ. MÉSZÁROS P. GÁLUSOVÁ T. SPIES N. LIBANTOVÁ J. MORAVČÍKOVÁ I.: Cd accumulation potential as a marker for heavy metal tolerance in soybean. Isr. J. Plant Sci. 62 2015 160-166.

  • YOKOYAMA R. NISHITANI K.: Genomic basis for cell-wall diversity in plants. A comparative approach to gene families in rice and Arabidopsis. Plant Cell Physiol. 45 2004 1111-1121.

Journal information
Impact Factor

CiteScore 2018: 0.68

SCImago Journal Rank (SJR) 2018: 0.173
Source Normalized Impact per Paper (SNIP) 2018: 0.288

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 263 89 3
PDF Downloads 129 60 3