Elicitation Phenolic Compounds in Cell Culture of Vitis vinifera L. by Phaeomoniella chlamydospora

Martin Sák 1 , 2 , Ivana Dokupilová 1 , 2 , Daniel Mihálik 3 , 4 , Jana Lakatošová 1 , 2 , Marcela Gubišová 3 , 5 , and Ján Kraic 3 , 6
  • 1 Research Institute of Viticulture and Enology, Hlavná ulica 326, SK-90041, Rovinka, Slovak Republic
  • 2 Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, SK-81237 Bratislava, Slovak Republic
  • 3 Research Institute of Plant Production, Bratislavská cesta 122, 921 68, Piešťany, Slovak Republic
  • 4 Institute of High Mountain Biology, University of Žilina, SK-05956 Tatranská Javorina 7, Slovak Republic
  • 5 Department of Botany and Genetics, Faculty of Natural Sciences, Constantine The Philosopher University, Tr. A. Hlinku 1, SK-94974 Nitra, Slovak Republic
  • 6 Department of Biotechnology, Faculty of Natural Sciences, University of SS. Cyril and Methodius, J. Herdu 2, SK-91701 Trnava, Slovak Republic


The in vitro cell cultures of Vitis vinifera L. cv. St. Laurent were treated with two elicitors - synthetic methyl jasmonate and natural, prepared from grapevine plant infected with the Phaeomoniella chlamydospora, the agent causing the Esca disease of grapevine. Efficiency of phenolic compounds production after elicitation of cell culture was analysed immediately after treatment (15 min, 30 min, 60 min) and later (after 24, 48, and 72 hours). The cell growth and content of phenolic compounds (+)-catechin, (-)-epicatechin, p-coumaric acid, syringaldehyde, rutin, vanillic acid, and trans-resveratrol were analysed in cultivated cells as well as in cultivation medium. Pch-treatment increased production of total polyphenols the most significantly 15 min after the elicitation and in optimal time was 2.86 times higher than in nonelicited culture and 1.44 times higher than in MeJa induced cell culture.

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • ABDULLAHIL BAQUE, M., MOH, S.-H., LEE, E.-J., ZHONG, J.-J., PAEK, K.-Y.: Production of biomass and useful compounds from adventitious roots of high-value added medicinal plants using bioreactor. Biotechnol. Adv., 30, 2012, 1255-1267

  • APOSTOLOU, A., STAGOS, D., GALITSIOU, E., SPYROU, A., HAROUTOUNIAN, S., PORTESIS, N., TRIZOGLOU, I., WALLACE HAYES, A., TSATSAKIS, A.M., KOURETAS, D.: Assessment of polyphenolic content, antioxidant activity, protection against ROS-induced DNA damage and anticancer activity of Vitis vinifera stem extracts. Food Chem. Toxicol., 61, 2013, 60-68.

  • BRUNO, G., SPARAPANO, G.: Effects of three esca-associated fungi on Vitis vinifera L.: I. Characterization of secondary metabolites in culture media and host responses to the pathogens in calli. Physiol. Mol. Plant Pathol., 69, 2006, 209-223.

  • CAI, Z., KNORR, D., SMETANSKA, I.: Enhanced anthocyanins and resveratrol production in Vitis vinifera cell suspension culture by indanoyl-isoleucine, Nlinolenoyl- l glutamine and insect saliva. Enzyme Microb. Technol., 50, 2012, 29-34.

  • CAI, Z., RIEDEL, H., SAW, N.M.M.T, MEWIS, I., REINEKE, K., KNORR, D., SMETANSKA, I.: Effects of elicitors and high hydrostatic pressure on secondary metabolism of Vitis vinifera suspension culture. Process Biochem., 46, 2011, 1411-1416.

  • DELGADO ADÁMEZ, J., GAMERO SAMINO, E., VALDÉS SÁNCHEZ, E., GONZÁLEZ-GÓMEZ, D.: In vitro estimation of the antibacterial activity and antioxidant capacity of aqueous extracts from grape-seeds (Vitis vinifera L.). Food Control, 24, 2012, 136-141.

  • DÖRNENBURG, H., KNORR, D.: Strategies for the improvement of secondary metabolite production in plant cell cultures. Enzyme Microb. Technol., 17, 1995, 674-684.

  • ESCORIAZA, G., SANSBERRO, P., GARCÍA-LAMPASONA, S., GATICA, M., BOTTINI, R., PICCOLI, P.: In vitro cultures of Vitis vinifera L. cv. Chardonnay synthesize the phytoalexin nerolidol upon infection by Phaeoacremonium parasiticum. Phytopath. Mediterranea, 52, 2013, 289-297.

  • FERNANDES, F., RAMALHOSA, E., PIRES, P., VERDIAL, J., VALENTÃO, P., ANDRADE, P., BENTO, A., PEREIRA, J.A.: Vitis vinifera leaves towards bioactivity. Ind. Crops Prod., 43, 2013, 434-440.

  • FRANCOIS, C., MARSHALL, R.D., NEUBERGER, A., Carbohydrates in proteins. 4. The determination of mannose in hen’s-egg albumin by radioisotope dilution. J. Biochem., 83, 1962, 335-341.

  • HIPPOLYTE, I., MARIN, B., BACCOU, J.C., JONARD, R.: Growth and rosmarinic acid production in cell suspension cultures of Salvia officinalis L. Plant Cell Rep., 11, 1992, 109-112.

  • IRITI, M., FAORO, F.: Grape phytochemicals: A bouquet of old and new nutraceuticals for human health. Med. Hypotheses, 67, 2006, 833-838.

  • JIMÉNEZ-APARICIO, A., GUTIÉRREZ-LÓPEZ, G.: Production of food related colorants by culture of plant cells. In: SHAHIDI, F., KOLODZIEJCZYK, P., WHITAKER, J.R., LOPEZ MUNGUIA, A., FULLER, G. (Eds.) Chemicals via higher plant bioengineering (Advances in experimental medicine and biology). Springer Science + Business Media, New York, 1999, 195-210.

  • LIANG, Z., OWENS, C.L., ZHONG, G.-Y., CHENG, L.: Polyphenolic profiles detected in the ripe berries of Vitis vinifera germplasm. Food Chem., 129, 2011, 940-950.

  • LIANG, Z., YANG, Y., CHENG, L., ZHONG, G.-Y.: Polyphenolic composition and content in the ripe berries of wild Vitis species, Food. Chem., 132, 2012, 730-738.

  • LIMA, M.R.M., DIAS, A.C.P.: Phaeomoniella chlamydospora - induced oxidative burst in Vitis vinifera cell suspensions: Role of NADPH oxidase and Ca2+. J. Phytopathol., 160, 2012, 129-134.

  • LIMA, M.R.M., FERRERES, F., DIAS, A.C.P.: Response of Vitis vinifera cell cultures to Phaeomoniella chlamydospora: changes in phenolic production, oxidative state and expression of defence-related genes. Eur. J. Plant Pathol., 132, 2012, 133-146.

  • LIU, W., LIU, C., YANG, C., WANGA, L., LI, S.: Effect of grape genotype and tissue type on callus growth and production of resveratrols and their piceids after UV-C irradiation. Food Chem., 122, 2010, 475-481.

  • LUINI, E., FLEURAT-LESSARD, P., ROUSSEAU, L., ROBLIN, G., BERJEAUD, J.-M.: Inhibitory effects of polypeptides secreted by the grapevine pathogens Phaeomoniella chlamydospora and Phaeoacremonium paleophilum on plant cell activities. Physiol. Mol. Plant Pathol., 74, 2010, 403-411.

  • MARTIN, N., VESENTINI, D., REGO, C., MONTEIRO, S. OLIVEIRA, H., BOAVIDA FERREIRA, R.: Phaeomoniella chlamydospora infection induces changes in phenolic compounds content in Vitis vinifera, Phytopathol. Mediterr., 48, 2009, 101-116.

  • MATKOWSKI, A.: Plant in vitro culture for the production of antioxidants - A review. Biotechnol. Adv., 26, 2008, 548-560.

  • MULABAGAL, V., TSAY, H.-S.: Plant cell cultures - An alternative and efficient source for the production of biologically important secondary metabolites. Int. J. Appl. Sci. Eng., 2, 2004, 29-48.

  • MURASHIGE, T., SKOOG, F.: Revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant., 15, 1962, 473-497.

  • RAO, S.R., RAVISHANKAR, G.A., Plant cell cultures: Chemical factories of secondary metabolites. Biotechnol. Adv., 20, 2002, 101-153.

  • RODRIGUEZ-BERNALDO DE QUIRÓS, A., LAGE-YUSTY, M.A., LÓPEZHERNÁNDEZ, J.: HPLC-analysis of polyphenolic compounds in Spanish white wines and determination of their antioxidant activity by radical scavenging assay. Food Res. Inter., 42, 2009, 1018-1022.

  • SANTOS, C., FRAGOEIRO, S., VALENTIM, H., PHILLIPS, A.: Phenotypic characterisation of Phaeoacremonium and Phaeomoniella strains isolated from grapevines: enzyme production and virulence of extra-cellular filtrate on grapevine calluses. Sci. Hort., 107, 2006, 123-130.

  • SAW, N.M.M.T., RIEDEL, H., CAI, Z., KÜTÜK, O., SMETANSKA, I.: Stimulation of anthocyanin synthesis in grape (Vitis vinifera) cell cultures by pulsed electric fields and ethephon. Plant Cell Tissue Organ Cult., 108, 2012, 47-54.

  • SPARAPANO, L., BRUNO, G., GRANITI, A.: Effects on plants of metabolites produced in culture by Phaeoacremonium chlamydosporum, P. aleophilum and Fomitiporia punctata. Phytopathol. Mediterr., 39, 2000, 169-177.

  • TASSONI, A., FORNALÈ, S., FRANCESCHETTI, M., MUSIANI, F., MICHAEL, A.J., PERRY, B.: Jasmonates and Na-orthovanadate promote resveratrol production in Vitis vinifera cv. Barbera cell cultures. New Phytol., 166, 2005, 895-905.

  • TAVARES, S., VESENTINI, D., FERNANDES, J.C., FERREIRA, R.B., LAUREANO, O., RICARDO-DA-SILVA, J.M., AMÂNCIO, S.: Vitis vinifera secondary metabolism as affected by sulfate depletion: Diagnosis through phenylpropanoid pathway genes and metabolites. Plant Physiol. Biochem., 66, 2013, 118-126.

  • ULBRICH, B., WIESNER, W., ARENS, H.: Large scale production of rosmarinic acid from plant cell cultures of Coleus blumei Benth. In: DEUS-NEUMANN, B., BARZ, W., REINHARD, E. (Eds.) Secondary metabolism of plant cell culture. Springer-Verlag, Berlin, 1985, 293-303.

  • WILLIAMSON, G., CARUGHI, A.: Polyphenol content and health benefits of raisins. Nutr. Res., 30, 2010, 511-519.

  • XU, J., GE, X., DOLANA, M.C.: Towards high-yield production of pharmaceutical proteins with plant cell suspension cultures. Biotechnol. Adv., 29, 2011, 278-299.

  • YU, L.-J., LAN,W.-Z., QIN, W.-M., XU, H.-B., Effects of salicylic acid on fungal elicitor-induced membrane-lipid peroxidation and taxol production in cell suspension cultures of Taxus chinensis. Process Biochem., 37, 2001, 477-482.

  • ZENK, M.H., EI-SHAGI, H., SCHULTE, U., Anthraquinone production by cell suspension cultures of Morinda citrifolia. Planta Medica Suppl., 75, 1975, 79-101.

  • ZHANG, W., CURTIN, C., KIKUCHI, M., FRANCO, C.: Integration of jasmonic acid and light irradiation for enhancement of anthocyanin biosynthesis in Vitis vinifera suspension cultures. Plant Sci., 162, 2002, 459-468.

  • ZHAO, J., LAWRENCE, T., DAVIS, C., VERPOORTE, R.: Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnol. Adv., 23, 2005, 283-333


Journal + Issues