Elicitation Phenolic Compounds in Cell Culture of Vitis vinifera L. by Phaeomoniella chlamydospora

Martin Sák 1 , 2 , Ivana Dokupilová 1 , 2 , Daniel Mihálik 3 , 4 , Jana Lakatošová 1 , 2 , Marcela Gubišová 3 , 5  und Ján Kraic 3 , 6
  • 1 Research Institute of Viticulture and Enology, Hlavná ulica 326, SK-90041, Rovinka, Slovak Republic
  • 2 Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, SK-81237 Bratislava, Slovak Republic
  • 3 Research Institute of Plant Production, Bratislavská cesta 122, 921 68, Piešťany, Slovak Republic
  • 4 Institute of High Mountain Biology, University of Žilina, SK-05956 Tatranská Javorina 7, Slovak Republic
  • 5 Department of Botany and Genetics, Faculty of Natural Sciences, Constantine The Philosopher University, Tr. A. Hlinku 1, SK-94974 Nitra, Slovak Republic
  • 6 Department of Biotechnology, Faculty of Natural Sciences, University of SS. Cyril and Methodius, J. Herdu 2, SK-91701 Trnava, Slovak Republic

Abstract

The in vitro cell cultures of Vitis vinifera L. cv. St. Laurent were treated with two elicitors - synthetic methyl jasmonate and natural, prepared from grapevine plant infected with the Phaeomoniella chlamydospora, the agent causing the Esca disease of grapevine. Efficiency of phenolic compounds production after elicitation of cell culture was analysed immediately after treatment (15 min, 30 min, 60 min) and later (after 24, 48, and 72 hours). The cell growth and content of phenolic compounds (+)-catechin, (-)-epicatechin, p-coumaric acid, syringaldehyde, rutin, vanillic acid, and trans-resveratrol were analysed in cultivated cells as well as in cultivation medium. Pch-treatment increased production of total polyphenols the most significantly 15 min after the elicitation and in optimal time was 2.86 times higher than in nonelicited culture and 1.44 times higher than in MeJa induced cell culture.

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