Distribution of Zinc and Cadmium in Tissues of Giant Reed (Arundo Donax L.): Sequential Extraction - Radiometric Study

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Heavy metals are taken up by the vascular plant root system from water solutions in cationic forms. Subsequently, during both short and long distance transport to other plant tissues, cation forms are incorporated to many bioorganic compounds differing in stability, ionic character and physico-chemical properties such as solubility in lipid structures and mobility across cell membrane systems. Many sequential and single step extraction methods have been elaborated for characterization of the role of individual components of plant cells components in transport and detoxication of heavy metals. In our study, dry biomass of giant reed (Arundo donax L.) grown in hydroponic media spiked with 65ZnCl2 and 109CdCl2 was treated with dithizone solutions as complexing ligand in order to convert free Zn2+ and Cd2+ ions to corresponding dithizonates. Treatment with dithizone showed that up to 67 % of the total plant Cd and 56 % of the total plant Zn were transformed to dithizonate complexes extracted with chloroform. Extraction of biomass with Folch reagent showed that up to 48 % of the total root cadmium and up to 18 % of the total shoot cadmium is bound in lipid fraction. Zinc was not found in lipid fraction of root and shoot. Derivatization of the dried root and shoot lipid fraction by dithizone showed that two third of Cd in root and practically all Cd in shoot lipid fraction could be transformed to Cd-dithizonate. Methods of biomass treating with complexing ligands and a method of sequential extraction procedures with non-polar organic solvents and radiotracer methodology seem to be useful methods for the study of metal speciation and distribution in vascular plants

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  • BABEL S. DACERA D.D.M.: Heavy metal removal from contaminated sludge for land application. J. Waste Manag. 26 2006 988-1004.

  • BONANNO G.: Arundo donax as a potential biomonitor of trace element contamination in water and sediment. Ecotoxicol. Environ. Saf. 80 2012 20-27.

  • BOTTARI E. FESTA M.R.: On the behavior of cysteine as ligand of cadmium (II). Talanta 44 1997 1705-1718.

  • BRACKLOW U. DREWS A. VOCKS M. KRAUME M.: Comparison of nutrients degradation in small-scale MBR Fed with synthetic/domestic wastewater. J. Hazard. Mater. 144 2007 620-626.

  • CREA F.: Formation and stability of phytate complexes in solutions. Coord. Chem. Rev. 252 2008 1108-1120.

  • DÜREŠOVÁ Z. HORNÍK M. GUBIŠOVÁ M. GUBIŠ J.: Phytofiltration potential of Arundo donax in Cd removing from contaminated wastewater. Chem. Pap. 2014 (accepted).

  • DÜREŠOVÁ Z. ŠUŇOVSKÁ A. HORNÍK M. PIPÍŠKA M. GUBIŠOVÁ M GUBIŠ J. AUGUSTÍN J.: Phytofiltration of Cd and Zn by root system of energy plants. Proceedings of the International conference of Applied Natural Sciences. Nový Smokovec Slovakia October 2-4 2013 26.

  • FOLCH J. LEES M. SLOANE STANLEY G.H.: A simple method for the isolation and purification of total lipides from animal tissues. J. Biol. Chem. 226 1957 497-509.

  • FRIŠTÁK V. VALOVČIAKOVÁ M. PIPÍŠKA M. AUGUSTÍN J.: Simultaneous and sequential extraction protocols as tools for determination of Zinc bioavailability in dried anaerobic sludge. Nova Biotechnol. Chim. 11 2012 167-175.

  • HAN Z. HU Z.: Tolerance of Arundo donax to heavy metals. Chin. J. Appl. Ecol. 16 2005 161-165.

  • HIROSHI K. HIDEYUKI I. TOMOYUKI O.: Mutual separation of metal ions by dithizone extraction-isotachophoresis on the basis of HSAB principle. Analyst 9 1994 723-726.

  • HOAGLAND D.R.: Optimum nutrient solutions for plants. Science 52 1920 562-564.

  • HORNÍK M. ŠUŇOVSKÁ A. PIPÍŠKA M. GUBIŠOVÁ M. GUBIŠ J. AUGUSTÍN J.: Phytofiltration of Cd and Zn by roots of vascular plants: Effect of metals speciation. Proceedings of International conference of Applied Natural Sciences 2011 Faculty of Natural Sciences UCM Trnava 2011 210-214.

  • CHRISTENSEN J.J IZATT M.R.: Handbook of metal-ligand heats and related thermodynamic quantities. Marcel Dekker New York 1983 783 pp.

  • LINDSAY W.L. W.A. NORVELL W.A.: Development of a DTPA soil test for zinc iron manganese and copper. Soil Sci. Soc. Amer. J. 42 1978 421-428.

  • MARTELL A.E.: Stability constants of metal-ion complexes. The Chemical Society London 1964 754 pp.

  • MAVROGIANOPOULOS G. VOGLI V. KYRISIS S.: Use of wastewater as a nutrient solution in a closed gravel hydroponic culture of giant reed (Arundo donax). Bioresour. Technol. 82 2002 103-107.

  • MEHLICH A.: Mehlich-3 soil test extractant: a modification of Mehlich-2 extractant. Commn. Soil Sci. Plant Anal. 15 1984 1409-1416.

  • MEHLICH A.: Determination of P Ca Mg K Na and NH4. North Carolina Soil Test Division Raleig. 1953. No 1-53.

  • MURASHIGE T. SKOOG F.: A revised media for rapid growth and bio assay with tobacco tissue cultures. Physiol. Plant. 15 1962 473-479.

  • PILU R. BUCCI A. BADONE F.C. LANDONI M.: Giant reed (Arundo donax L.): A weed plant or a promising energy crop? Afr. J. Biotechnol. 11 2012 9163-9174.

  • SOLOV´EV V. SUKHNO I. BUZKO V. POLUSHIN A. MARCOU G. TSIVADZE A. VARNEK A.: Stability constants of complexes of Zn2+ Cd2+ and Hg2+ with organic ligands: QSPR consensus modeling and design of new metal binders. J. Incl. Phenom. Macrocycl. Chem. 72 2012 309-321.

  • TAKAHASHI W. TAKAMIZO T. KOBAYAHI M. EBINA M.: Plant regeneration from calli in Giant Reed (Arundo donax L). Grassland Sci. 4 2010 56224-56229.

  • TESSIER A. CAMPBELL P.G.C. BISSON M.: Sequential Extraction Procedure for the Speciation of Particulate Trace Metals. J. Anal. Chem. 51 1979 844-851.

  • URE A.M. QUEVAUVILLER P. MUNTAU H. GRIEPINK B.: Speciation of heavy metals in soils and sediments. An account of the improvement and harmonization of extraction techniques undertaken under the auspices of the BCR of the commission of the European communities. Int. J. Environ. Anal. Chem. 51 1993 1-4.

  • VAN HULLEBUSCH E.D. UTOMO S. ZANDVOORT M.H. LENS P.N.L.: Comparison of three sequential extraction procedures to describe metal fractionation in anaerobic granular sludges. Talanta 65 2005 549-558.

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