The Effect of Culture Age and Initial Silver Concentration on Biosynthesis of Ag Nanoparticles

Open access


Many organisms or their extracts have the ability to reduce Ag+ ions to Ag0 and stabilize them what results in nanoparticle formation in solution. The aim of the article was to study the influence of two selected parameters - initial silver concentration and culture age, on Ag nanoparticles production by green algae Parachlorella kessleri. The presence of Ag nanoparticles in the solution was confirmed by the UV-vis spectroscopy and TEM analyses. Typical curve with the peak at app. 420 nm was found for nanoparticles produced by algae. While culture age did not have any significant effect, the initial silver concentration had significant influence on nanoparticle production which influenced the rate of nanoparticle production, their amount, their size and stability, as well

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • BANKURA K.P. MAITY D. MOLLICK M.M.R. MONDAL D. BHOWMICK B. BAIN M.K. CHAKRABORTY A. SARKAR J. ACHARYA K. CHATTOPADHYAY D.: Synthesis characterization and antimicrobial activity of dextran stabilized silver nanoparticles in aqueous medium. Carbohydr. Polym. 89 2012 1159- 1165

  • CASTRO L. BLÁZQUEZ M.L. GONZÁLEZ F. MUÑOZ J.A. BALLESTER A.: Extracellular biosynthesis of gold nanoparticles using sugar beet pulp. Chem. Eng. J. 164 2010 92-97.

  • CASTRO L. BLÁZQUEZ M.L. MUÑOZ J.A. GONZÁLEZ F. BALLESTER A.: Biological synthesis of metallic nanoparticles using algae. IET Nanobiotechnol. 2013 1-8.

  • CHAKRABORTY N. BANERJEE A. LAHIRI S. PANDA A. GHOSH A.N. PAL R.: Biorecovery of gold using cyanobacteria and an eukaryotic alga with special reference to nanogold formation-a novel phenomenon. J. App. Phyc. 21 2009 145-152.

  • DAHOUMANE S.A. WIJESEKERA K. FILIPE C.D.M. BRENNAN J.D.: Stoichiometrically controlled production of bimetallic Gold-Silver alloy colloids using micro-alga cultures. J. Coll. Interf. Sci. 416 2014 67-72.

  • DAVENAS J. LTAIEF A. BARLIER V. BOITEUX G. BOUAZIZI A.: nanomaterials for photovoltaic conversion. Mater. Sci. Eng. C. 28 5-6 2008 744-750.

  • DUBAS S.T. PIMPAN V.: Green synthesis of silver nanoparticles for ammonia sensing. Talanta 76 2008 29-33.

  • FAYAZ A. M. GIRILAL M. RAHMAN M. VENKATESAN R. KALAICHELVAN P.T.: Biosynthesis of silver and gold nanoparticles using thermophilic bacterium Geobacillus stearothermophilus. Process Biochem. 46 2011 1958-1962

  • FRITSCHE W. PORWOL H. WIEGAND A.: In-situ formation of Ag containing nanoparticles in thin polymer films. Nanostruct. Mater. 10 1 1998 71-89

  • GOVINDARAJU K. BASHA S.K. KUMAR V.G.. SINGARAVELU G.: Silver gold and bimetallic nanoparticles production using single-cell protein (Spirulina platensis) Geitler. J. Mater. Sci. 43 2008 5115-5122.

  • GURUNATHAN S. KALISHWARALAL K. VAIDYANATHAN R. DEEPAK V. PANDIAN S.R.K. MUNIYANDI J. HARIHARAN N. EOM S.H.: Biosynthesis purification and characterization of silver nanoparticles using Escherichia coli Coll. Surf. B: Biointerfaces 74 2009 328-335.

  • JENA J. PRADHAN N. DASH B.P. SUKLA L.B. PANDA P.K.: Biosynthesis and characterization of silver nanoparticles using microalga Chlorococcum humicola and its antibacterial activity. Int. J. Nanomat. Biostruct. 3 1 2013 1-8.

  • KORBEKANDI H. IRAVANI S. ABBASI S.: Production of nanoparticles using organisms. Crit. Rev. Biotechnol. 29 4 2009 279-306.

  • LI X. XU H. CHEN Z. CHEN G.: Biosynthesis of nanoparticles by microorganisms and their applications. J. Nanomater. 2011 Art. ID 270974 16 pp. doi:10.1155/2011/270974

  • LIN H-W. HWU W-H. GER M-D.: The dispersion of silver nanoparticles with physical dispersal procedures. J. Mater. Process Technol. 206 2008 56-61.

  • LOGESWARI P. SILAMBARASAN S. ABRAHAM J.: Synthesis of silver nanoparticles using plants extract and analysis of their antimicrobial property. J. Saudi Chem. Soc. 2013 (in press) MANEERUNG T. TOKURA S. RUJIRAVANIT R.: Impregnation of silver nanoparticles into bacterial cellulose for antimicrobial wound dressing. Carbohydr. Polym. 72 1 2008 43-51.

  • MUKUNTHAN K.S. ELUMALAI E.K. PATEL T.N. MURTY V.R.: Catharanthus roseus: a natural source for the synthesis of silver nanoparticles. Asian Pac. J. Trop. Biomed. 2011 270-274

  • PETIT C. LIXON P. PILENI M-P.: In situ synthesis of silver nanocluster in AOT reverse micelles. J. Pys. Chem. 97 1993 12974-12983.

  • PINTO V.V. FERREIRA M.J. SILVA R. SANTOS H.A. SILVA F. PEREIRA C.M.: Long time efect on the stability of silver nanoparticles in aqueous medium: Effect of the synthesis and storage conditions. Coll. Surf. A: Physicochemical and Engineering Aspects 364 2010 19-25.

  • ROOPAN S.M. MADHUMITHA R.G. RAHUMAN A.A. KAMARAJ C. BHARATHIA A. SURENDRA T.V.: Low-cost and eco-friendly phyto-synthesis of silver nanoparticles using Cocos nucifera coir extract and its larvicidal activity. Ind. Crops Prod. 43 2013 631- 635.

  • RUPARELIA J.P. CHATTERJEE A.K. DUTTAGUPTA S.P. MUKHERJI S.: Strain specificity in antimicrobial activity of silver and copper nanoparticles. Acta Biomater. 4 2008 707-716

  • SANGHI R. VERMA P.: Biomimetic synthesis and characterization of protein capped silver nanoparticles. Bioresour. Technol. 100 2009 501-504.

  • SHALIGRAM N.S. BULE M. BHAMBURE R. SINGHAL R.S. SINGH S.K. SZAKACS G. PANDEY A.: Biosynthesis of silver nanoparticles using aqueous extract from the compactin producing fungal strain. Process Biochem. 44 2009 939-943

  • SHARMA B. PURKAYASTHA D.D. HAZRA S. GOGOI L. BHATTACHARJEE CH. R. GHOSH N.N. ROUT J.: Biosynthesis of gold nanoparticles using freshwater green alga Prasiola crispa. Mat. Lett. 116 2014 94-97.

  • SHARMA K.V. YNGARD R.A. LIN Y.: Silver nanoparticles: Green synthesis and their antimicrobial activities. Adv. Colloid. Interface Sci. 145 2009 83-96.

  • SONG J.Y. KIM B.S.: Rapid biological synthesis of silver nanoparticles using plant leaf extracts. Bioprocess Biosyst. Eng. 32 2009 79-84. XIE J. LEE J.Y. WANG D.I.C. TING Y.P.: Silver nanoplates: From biological to biomimetic synthesis. ACS Nano 1 5 2007 429-439.

  • YAN J. CHEN J.: Colloidal nanosilver solution and method for making the same. US Patent US 2003/0185889. 2003 6pp.

Journal information
Impact Factor

CiteScore 2018: 0.68

SCImago Journal Rank (SJR) 2018: 0.173
Source Normalized Impact per Paper (SNIP) 2018: 0.288

Cited By
All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 287 100 2
PDF Downloads 115 70 3