The Effect of Culture Age and Initial Silver Concentration on Biosynthesis of Ag Nanoparticles

Jana Kaduková 1 , Oksana Velgosová 1 , Anna Mražíková 1 , and Renáta Marcinčáková 1
  • 1 Department of Material Science, Faculty of Metallurgy, Technical University of Kosice, Letna 9, 042 00 Kosice, Slovakia


Many organisms or their extracts have the ability to reduce Ag+ ions to Ag0 and stabilize them what results in nanoparticle formation in solution. The aim of the article was to study the influence of two selected parameters - initial silver concentration and culture age, on Ag nanoparticles production by green algae Parachlorella kessleri. The presence of Ag nanoparticles in the solution was confirmed by the UV-vis spectroscopy and TEM analyses. Typical curve with the peak at app. 420 nm was found for nanoparticles produced by algae. While culture age did not have any significant effect, the initial silver concentration had significant influence on nanoparticle production which influenced the rate of nanoparticle production, their amount, their size and stability, as well

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  • BANKURA, K.P., MAITY, D., MOLLICK, M.M.R., MONDAL, D., BHOWMICK, B., BAIN, M.K., CHAKRABORTY, A., SARKAR, J., ACHARYA, K., CHATTOPADHYAY, D.: Synthesis, characterization and antimicrobial activity of dextran stabilized silver nanoparticles in aqueous medium. Carbohydr. Polym., 89, 2012, 1159- 1165

  • CASTRO, L., BLÁZQUEZ, M.L., GONZÁLEZ, F., MUÑOZ, J.A., BALLESTER, A.: Extracellular biosynthesis of gold nanoparticles using sugar beet pulp. Chem. Eng. J., 164, 2010, 92-97.

  • CASTRO, L., BLÁZQUEZ, M.L., MUÑOZ, J.A., GONZÁLEZ, F., BALLESTER, A.: Biological synthesis of metallic nanoparticles using algae. IET Nanobiotechnol., 2013, 1-8.

  • CHAKRABORTY, N., BANERJEE, A., LAHIRI, S., PANDA, A., GHOSH, A.N., PAL, R.: Biorecovery of gold using cyanobacteria and an eukaryotic alga with special reference to nanogold formation-a novel phenomenon. J. App. Phyc., 21, 2009, 145-152.

  • DAHOUMANE, S.A., WIJESEKERA, K., FILIPE, C.D.M., BRENNAN, J.D.: Stoichiometrically controlled production of bimetallic Gold-Silver alloy colloids using micro-alga cultures. J. Coll. Interf. Sci., 416, 2014, 67-72.

  • DAVENAS, J., LTAIEF, A., BARLIER, V., BOITEUX, G., BOUAZIZI, A.: nanomaterials for photovoltaic conversion. Mater. Sci. Eng. C., 28, 5-6, 2008, 744-750.

  • DUBAS, S.T., PIMPAN, V.: Green synthesis of silver nanoparticles for ammonia sensing. Talanta, 76, 2008, 29-33.

  • FAYAZ, A. M., GIRILAL, M., RAHMAN, M., VENKATESAN, R., KALAICHELVAN, P.T.: Biosynthesis of silver and gold nanoparticles using thermophilic bacterium Geobacillus stearothermophilus. Process Biochem., 46, 2011, 1958-1962

  • FRITSCHE, W., PORWOL, H., WIEGAND, A.: In-situ formation of Ag containing nanoparticles in thin polymer films. Nanostruct. Mater., 10, 1, 1998, 71-89

  • GOVINDARAJU, K., BASHA, S.K., KUMAR, V.G.., SINGARAVELU, G.: Silver, gold and bimetallic nanoparticles production using single-cell protein (Spirulina platensis) Geitler. J. Mater. Sci., 43, 2008, 5115-5122.

  • GURUNATHAN, S., KALISHWARALAL, K., VAIDYANATHAN, R., DEEPAK, V., PANDIAN, S.R.K., MUNIYANDI, J. HARIHARAN, N., EOM, S.H.: Biosynthesis, purification and characterization of silver nanoparticles using Escherichia coli, Coll. Surf. B: Biointerfaces, 74, 2009, 328-335.

  • JENA, J., PRADHAN, N., DASH, B.P., SUKLA, L.B., PANDA, P.K.: Biosynthesis and characterization of silver nanoparticles using microalga Chlorococcum humicola and its antibacterial activity. Int. J. Nanomat. Biostruct., 3, 1, 2013, 1-8.

  • KORBEKANDI, H., IRAVANI, S., ABBASI, S.: Production of nanoparticles using organisms. Crit. Rev. Biotechnol. 29, 4, 2009, 279-306.

  • LI, X., XU, H., CHEN, Z., CHEN, G.: Biosynthesis of nanoparticles by microorganisms and their applications. J. Nanomater., 2011, Art. ID 270974, 16 pp. doi:10.1155/2011/270974

  • LIN H-W., HWU W-H., GER M-D.: The dispersion of silver nanoparticles with physical dispersal procedures. J. Mater. Process Technol. 206, 2008, 56-61.

  • LOGESWARI, P., SILAMBARASAN, S., ABRAHAM, J.: Synthesis of silver nanoparticles using plants extract and analysis of their antimicrobial property. J. Saudi Chem. Soc. 2013, (in press) MANEERUNG, T., TOKURA, S., RUJIRAVANIT, R.: Impregnation of silver nanoparticles into bacterial cellulose for antimicrobial wound dressing. Carbohydr. Polym. 72, 1, 2008, 43-51.

  • MUKUNTHAN, K.S., ELUMALAI, E.K., PATEL, T.N., MURTY, V.R.: Catharanthus roseus: a natural source for the synthesis of silver nanoparticles. Asian Pac. J. Trop. Biomed., 2011, 270-274

  • PETIT, C., LIXON, P., PILENI, M-P.: In situ synthesis of silver nanocluster in AOT reverse micelles. J. Pys. Chem., 97, 1993, 12974-12983.

  • PINTO, V.V., FERREIRA, M.J., SILVA, R., SANTOS, H.A., SILVA, F., PEREIRA, C.M.: Long time efect on the stability of silver nanoparticles in aqueous medium: Effect of the synthesis and storage conditions. Coll. Surf. A: Physicochemical and Engineering Aspects, 364, 2010, 19-25.

  • ROOPAN, S.M., MADHUMITHA, R.G., RAHUMAN, A.A., KAMARAJ, C., BHARATHIA, A., SURENDRA, T.V.: Low-cost and eco-friendly phyto-synthesis of silver nanoparticles using Cocos nucifera coir extract and its larvicidal activity. Ind. Crops Prod., 43, 2013, 631- 635.

  • RUPARELIA J.P., CHATTERJEE, A.K., DUTTAGUPTA, S.P., MUKHERJI, S.: Strain specificity in antimicrobial activity of silver and copper nanoparticles. Acta Biomater., 4, 2008, 707-716

  • SANGHI, R., VERMA, P.: Biomimetic synthesis and characterization of protein capped silver nanoparticles. Bioresour. Technol., 100, 2009, 501-504.

  • SHALIGRAM, N.S., BULE, M., BHAMBURE, R., SINGHAL, R.S., SINGH, S.K., SZAKACS, G., PANDEY, A.: Biosynthesis of silver nanoparticles using aqueous extract from the compactin producing fungal strain. Process Biochem., 44, 2009, 939-943

  • SHARMA, B., PURKAYASTHA, D.D., HAZRA, S., GOGOI, L., BHATTACHARJEE, CH. R., GHOSH, N.N., ROUT, J.: Biosynthesis of gold nanoparticles using freshwater green alga, Prasiola crispa. Mat. Lett., 116, 2014, 94-97.

  • SHARMA, K.V., YNGARD, R.A., LIN, Y.: Silver nanoparticles: Green synthesis and their antimicrobial activities. Adv. Colloid. Interface Sci., 145, 2009, 83-96.

  • SONG, J.Y., KIM, B.S.: Rapid biological synthesis of silver nanoparticles using plant leaf extracts. Bioprocess Biosyst. Eng., 32, 2009, 79-84. XIE, J., LEE, J.Y., WANG, D.I.C., TING, Y.P.: Silver nanoplates: From biological to biomimetic synthesis. ACS Nano, 1, 5, 2007, 429-439.

  • YAN, J., CHEN, J.: Colloidal nanosilver solution and method for making the same. US Patent US 2003/0185889. 2003, 6pp.


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