A New Approach to Identification of Biomarkers for Early Cancer Stage Detection

Open access


Gas chromatography and mass spectrometry (GC/MS) was applied for determination of concentrations volatile organic compounds present in human breath samples. The technique allows to rapid determination compounds in human air, at the level of parts per billion. It showed linear correlations ranging from 0.83-234.05 ppb, limit detection in the range of 0.31-0.75 ppb and precision, expressed as RSD, was less then 10.00%. Moreover, trained dogs are able to discriminate breath samples of patients with diagnosed cancer disease. We found positive correlation between dog indications and content of ethyl acetate and 2- pentanone in breath (r=0.85 and r=0.97, respectively)

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • BAKER R.R.: Smoke generation inside a burning cigarette: Modifying combustion to develop cigarettes that may be less hazardous to health. Prog. Energy Combust. Sci. 32 2006 373-385.

  • BUSZEWSKI B. RUDNICKA J. LIGOR T. WALCZAK M. JEZIERSKI T. AMANN A. Analytical and unconventional methods of cancer detection using odor. TrAC 38 2012 1-12.

  • COLLINS L.G. HAINES C. PERKEL R. ENCK R.E.: Lung cancer: diagnosis and mamagement. Am. Fam. Physician 75 2006 56-63.

  • DENG C. ZHANG J. XIAOFENG Y. ZHANG W. ZHANG X.: Determination of acetone in human breath by gas chromatography-mass spectrometry and solid phase microextraction with on fiber Derivatization. J. Chromatogr. B 810 2004 269-275.

  • HORVATH G. JARVERUD G.A. JARVERUD S. HORVATH I.: Human ovarian carcinomas detected by specific odor. Integr. Cancer Ther. 7 2008 76-80.

  • HRYNIUK A. ROSS B.M.: Detection of acetone and isoprene in human breath using a combination of thermal desorption and selected ion flow tube mass spectrometry. Int. J. Mass Spectrom. 285 2009 26-30.

  • LIBARDONI M. STEVENS P.T. WAITE J. SACKS R.: Analysis of human breath samples with a multi-bed sorption trap and comprehensive two-dimensional gas chromatography (GC×GC). J. Chromatogr. B 842 2006 13-21.

  • MCCULLOCH M. JEZIERSKI T. BROFFMAN M. HUBBARD A. TURNER K. JANECKI T. Diagnostic accuracy of canine scent detection in early- and latestage lung and breast cancers. Integr. Cancer Ther. 5 2006 30-39.

  • MCWILLIAMS A. LAM S.: New Approaches to Lung Cancer Prevention. Curr. Oncol. Rep. 4 2002 487-494.

  • PICKEL D. MANUCY G.P. WALKER D.B. HALL S.B. WALKER J.C.: Evidence for canine olfactory detection of melanoma. Appl. Anim. Behav. Sci. 89 2004 107-116.

  • PRADO C. MARTIN P. PERIAGO J.F.: Application of solid phase microextraction and gas chromatography-mass spectrometry to the determination of volatile organic compounds in end-exhaled breath samples. J. Chromatogr. A 1011 2003 125-134.

  • SPANEL P. ROLFE P. RAJAN B. SMITH D.: The selected ion flow tube (SIFT)-a novel technique for biological monitoring. Ann. Occup. Hyg. 40 1996 615-626.

  • WARNEKE C. KUCZYNSKI J. HANSEL A. JORDAN A. VOGEL W. LINDINGER W.: Proton transfer reaction mass spectrometry (PTR-MS): propanol in human breath. Int. J. Mass Spectrom. Ion Processes 154 1996 61-70.

  • WILLIS C.M. CHURCH S.M. GUEST C.M. COOK W.A. MCCARTHY N. BRANSBURY A.J. CHURCH M.R.T. CHURCH J.C.T.: Olfactory detection of human bladder cancer by dogs: proof of principle study Brit. Med. J. 329 2004 712-715.

  • YAO S. VENTURA D. PETRIBOIS C.: Analytical characterization of cell- asbestos fiber interactions in lung pathogenesis. Anal. Bioanal. Chem. 397 2010 2079-2089.

  • YU H. XU L. WANG P.: Solid phase microextraction for analysis of alkanes and aromatic hydrocarbons in human breath. J. Chromatogr. B 826 2005 69-74.

Journal information
Impact Factor

CiteScore 2018: 0.68

SCImago Journal Rank (SJR) 2018: 0.173
Source Normalized Impact per Paper (SNIP) 2018: 0.288

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 293 95 0
PDF Downloads 105 45 0