Optimization of the Antioxidant Extraction from Eleutherococcus Senticosus Roots by Response Surface Methodology


Eleutherococcus senticosus is known as adaptogen with benefits in general health promotion. The aim of this study was to investigate the effect of major extraction parameters on extraction yield of antioxidants measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity. Secondly, content of total polyphenols was evaluated. Optimal conditions of the extraction were processed by response surface methodology. The independent variables of extraction were proposed as temperature, solid-liquid ratio and solvent composition. For the optimal antioxidant extraction, E. senticosus is suitable to extract by 23 % (v/v) aqueous ethanol at 70 °C in ratio 53 mL of extraction solvent per g of plant material. The optimal conditions calculated for the extraction of total polyphenols were very similar (70 °C, 22 % (v/v) aqueous ethanol) expect solid-liquid ratio which indicates need of increasing of solid-liquid ratio to 91 mL of extraction solvent per g of plant material

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  • BOX, G.E.P., WILSON, K.B.: On the experimental attainment of optimum conditions. J. R. Stat. Soc. Series B, 13, 1951, 1-45.

  • CHEN, C.Y.O., RIBAYA-MERCADO, J.D., MCKAY, D.L., CROOM, E., BLUMBERG, J.B.: Differential antioxidant and quinone reductase inducing activity of American, Asian and Siberian ginseng. Food Chem., 119, 2010, 445-451.

  • DAVYDOV, M., KRIKORIAN, A. D.: Eleutherococcus senticosus (Rupr. & Maxim.) Maxim. (Araliaceae) as an adaptogen: a closer look. J. Ethnopharmacol., 72, 2000, 345-393.

  • FUJIKAWA, T., YAMAGUCHI, A., MORITA, I., TAKEDA, H., NISHIBE, S.: Protective effects of Acanthopanax senticosus Harms from Hokkaido and its components on gastric ulcer in restrained cold water stressed rats. Biol. Pharm. Bull., 19, 1996, 1227-1230.

  • HIBASAMI, H., FUJIKAWA, T., TAKEDA, H., NISHIBE, S., SATOH, T., FUJISAWA, T., NAKASHIMA, K.: Induction of apoptosis by Acanthopanax senticosus HARMS and its component, seamin in human stomach cancer KATO III cells. Oncol. Rep., 7, 2000, 1213-1216.

  • JUNG, H.J., PARK, H.J., KIM, R.G., SHIN, K.M., HA, J., CHOI, J.W., KIM, H.J., LEE, Y.S., LEE, K.T.: In vivo anti-inflammatory and antiociceptive effects of liriodendrin isolated from the stem bark of Acanthopanax senticosus. Planta Med., 69, 2003, 610-616.

  • KIM, L.H., HAN, S.S., CHOI, Y.S.: Antioxidant effects of the extracts of Acanthopanax senticosus. Korean J. Pharmacogn., 32, 2002, 359-363.

  • KITTS, D., HU, C.: Efficacy and safety of ginseng. Public Health Nutr., 3, 2000, 473-485.

  • LEE, B.M., LEE, S.K., KIM, H.S.: Inhibition of oxidative DNA damage, 8-OHdG, and carbonyl contents in smokers treated with antioxidants (vitamin E, vitamin C, beta-carotene and red ginseng). Cancer Lett., 132, 1998, 219-227.

  • LEE, S., SON, D., RYU, J., LEE, Y.S., JUNG, S.H., KANG, J., LEE, S.Y., KIM, H.S., SHIN, K.H.: Anti-oxidant activities of Acanthopanax senticosus stems and their lignan components. Arch. Pharm. Res., 27, 2004, 106-110.

  • LEE, S.R., SHIN, H.H., JEONG, J.H., HWANG, K.T., KIM, T.Y.: Effect of ethanol concentrations and extraction time on acanthoside-D and total polyphenol contents and antioxidant activities in ethanol extracts of eleuthero. J. Med. Plant Res., 24, 2011, 5700-5705.

  • LEE, Y.J., CHUNG, H.Y., KWAK, H.K., YOON, S.: The effects of A. senticosus supplementation on serum lipid profiles, biomarkers of oxidative stress and lymphocyte DNA damage in postmenopausal women. Biochem. Biophys. Res. Commun., 375, 2008, 44-48.

  • LI, Q., JIA, Y., XU, L., WANG, X., SHEN, Z., LIU, Y., BI, K.: Simultaneous determination of protocatechuic acid, syringin, chlorogenic acid, caffeic acid, liriodendrin and isofraxidin in Acanthopanax senticosus Harms by HPLC-DAD. Biol. Pharm. Bull., 29, 2006, 532-534.

  • MYERS, R.H., MONTGOMERY, D.C.: Response surface methodology: Process and product optimization using designed experiments. Wiley, New York, 1995, 704 pp.

  • NAVAL, M.V., GOMÉZ-SERRANILLOS, M.E., CARRETERO, M.E., VILLAR, A.M.: Neuroprotective effect of a ginseng (Panax ginseng) root extract on astrocytes primary culture. J. Ethnopharmacol., 112, 2007, 262-270.

  • PARK, H.R., PARK, E., RIM, A.R., JEON, K.I., HWANG, J.H., LEE, S.C.: Antioxidant activity of extracts from Acanthopanax senticosus. Afr. J. Biotechnol., 5, 2006, 2388-2396.

  • SINGLETON, V.L., ORTHOFER, R., LAMUELA-RAVENTOS, R.M.: Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin- Ciocalteu reagent. Methods Enzymol., 299, 1999, 152-178.

  • WANG, B.: Antioxidant activity of Acanthopanax senticosus extract and derivatives. Adv. Mat. Res., 531, 2012, 524-527.

  • WU, X., BEECHER, G.R., HOLDEN, J.M., HAYTOWITZ, D.B., GEBHARDT, R.L., PRIOR, R.L.: Lipophilic and hydrophilic antioxidant capacities of common foods in the United States. J. Agric. Food Chem., 52, 2004, 4026-4037.

  • XIANG, Y.Z., SHANG, H.C., GAO, X.M., ZHANG, B.L.: A comparison of the ancient use of ginseng in traditional Chinese medicine with modern pharmacological experiments and clinical trials. Phytother. Res., 22, 2008, 851-858.

  • YEN, G.C., CHEN, H.Y.: Antioxidant activity of various tea extracts in relation to their antimutagenicity. J. Agric. Food Chem., 43, 1995, 27-32.

  • YI, J.M., HONG, S.H., KIM, J.H., KIM, H.K., SONG, H.J., KIM, H.M.: Effect of Acanthopanax senticosus stem on mast cell-dependent anaphylaxis. J. Ethnopharmacol., 79, 2002, 347-352.


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