The group selective molecularly imprinted polymers (MIPs) for coumarins, including umbelliferone, herniarin, 4-methylumbelliferone, scoparone were developed. Using umbelliferone as the template molecule, methacrylic acid as functional monomer, ethylene glycol dimethacrylate as linking agent, chloroform as porogen and bulk polymerization as synthetic method, the MIPs were synthesized and characterized with rebinding experiments. The characteristics of MIPs were evaluated by chromatographic method and frontal analysis, and demonstrating good selectivity and high binding capacity (269 μg of umbelliferone per 100 mg of polymer). The group selective MIP was used as sorbent for the SPE pretreatment of coumarins from propolis extracts prior to HPLC analysis. Analysis of the samples showed good recoveries (>70 %). The limits of quantitation (LOQs) for studied compounds were 0.3-10 ng.mL-1 (determined for fluorescence detection).
If the inline PDF is not rendering correctly, you can download the PDF file here.
CIEŚLA Ł. WAKSMUNDZKA-HAJNOS M.: Two-dimensional thin-layer chromatography in the analysis of secondary plant metabolites. J. Chromatogr. A 1216 2009 1035-1052.
FERNÁNDEZ IZQUIERDO M.E. QUESADA GRANADOS J. VILLALÓN MIR M. LÓPEZ MARTINEZ M.C.: Comparison of methods for determining coumarins in distilled beverages. Food Chem. 70 2000 251-258.
FONSECA F.N. TAVARES M.F.M. HORVÁTH C.: Capillary electrochromatography of selected phenolic compounds of Chamomilla recutita. J. Chromatogr. A 1154 2007 390-399.
HROBOŇOVÁ K. ČIŽMÁRIK J. LEHOTAY J. SPIŠSKÁ Ľ.: Examination of the chemical composition of propolis VIII. Extraction of coumarins from propolis samples. (in Slovak) Farm. Obzor 82 2013 17-21.
IKEDA R. WADA M. NISHIGAKI T. NAKASHIMA K.: Quantitation of coumarin derivatives in Noni (Morinda citrifilia) and their contribution of guenching effect on reactive oxygen species. Food Chemistry 113 2009 1169-1172.
JURD L. KING A. D. JR. MIHARA K.: Antimicrobial properties of umbelliferone derivatives. Phytochemistry 10 1971 2965-2970.
MALIK A. KUSHNOOR A. SAINI V. SINGHAL S. KUMAR S. YADAV Y.C.: Analytical methods development of nutraceutical: Umbelliferone. Pharma Science monitor. Int. J. Pharm. Sci. 3 2012 67-73.
MICKE G.A. MORAES E.P. FARAH J.P.S. TAVARES M.F.M.: Assessing the separation of neutral plant secondary metabolites by micellar electrokinetic chromatography. J. Chromatogr. A 1004 2003 131-143.
NOVÁKOVÁ L. VILDOVÁ A. MATEUS P.J. GONCALVES T. SOLICH P.: Development and application of UHPLC - MS/MS method for the determination of phenolic compounds in Chamomile flowers and Chamomile tea extracts. Talanta 82 2010 1271 - 1280.
SFORCIN J.M. BANKOVA V.: Propolis: is there a potential for the development of new drugs. Rewiev. J. Ethnopharmacology 133 2011 253-260.
SILICI S. KUTLUCA S.: Chemical composition and antibacterial activity of propolis collected by three different races of honeybees in the same region. J. Ethnopharmacol. 99 2005 69-73.
SU J. ZHANG CH. ZHANG W. SHEN Y. LI H. LIU R. ZHANG X. HU X. ZHANG W.: Qualitative and quantitative determination of the major coumarins in Zushima by high performance liquid chromatography with diode array detector and mass spectrometry. J. Chromatogr. A 1216 2009 2111 - 2117.
TAMAYO F.G. TURIEL E. MARTÍN-ESTEBAN A.: Molecularly imprinted polymers for solid-phase extraction and solid-phase microextraction: Recent developments and future trends. J. Chromatogr. A 1152 2007 32-40.
WANG W. TANG J. WANG S. ZHOU L. HU Z.: Method development for the determination of coumarin compounds by capillary electrophoresis with indirect laser-induced fluorescence detection. J. Chromatogr. A 1148 2007 108-114.
ZHOU T. XIAO X. LI G. ZONG-WEI C.: Study of polyethylene glycol as green solvent in the microwave - assisted extraction of flavone and coumarin compounds from medicinal plants. J. Chromatogr. A 1218 2011 3608 - 3615.