Determination of the Functional Groups in Algae Parachlorella Kessleri by Potentiometric Titrations

Abstract

The acidic functional groups of the cell wall of native algae Parachlorella kessleri were evaluated by potentiometric titrations. The Gran´s method was applied to determination of the total, strong, weak and very weak acidities. The total organic acidity obtained for biomass was 3.93 mmol g-1, the largest content belonged to the strong acidic groups (2.13 mmol g-1) together with the weak acidic carboxylic groups (1.28 mmol g-1). Very weak acidities represented by the amine groups (0.52 mmol g-1) did not exceed 14% and they formed the lowest numerous part of all acidic functional groups.

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • AHMADY-ASBCHIN, S., ANDRES, Y., GÉRENTE, C., LE CLOIREC, P.: Biosorption of Cu(II) from aqueous solution by Fucus serratus: Surface characterization and sorption mechanisms. Bioresour. Technol., 99, 2008, 6150- 6155.

  • ARAVINDHAN, R., RAO, J.R., NAIR, B.U.: Removal of basic yellow dye from aqueous by sorption on green alga Caulerpa scalpelliformis. J. Hazard. Mater., 142, 2007, 68-76.

  • BRUNELOT, G., ADRIAN, P., ROUILLER, J., GUILLET, B., ANDREUX, F.: Determination of dissociable acid groups of organic compounds extracted from soils, using automated potentiometric titration. Chemosphere, 19, 1989, 1413- 1419.

  • DAVIS, T.A., VOLESKY, B., VIEIRA, R.H.S.F.: Sargassum seaweed as biosorbent for heavy metals. Water Res., 34, 2000, 4270-4278.

  • DAVIS, T.A., VOLESKY, B., MUCCI, A.: A review of the biochemistry of heavy metal biosorption by brown algae. Water Res., 37, 2003, 4311-4330.

  • GRAN, G.: Determination of the equivalence point in potentiometric titrations. Part II, Analyst, 77, 1952, 661-671.

  • GRIMM, A., ZANZI, R., BJÖRNBOM, E., CUKIERMAN, A.L.: Comparison of different types of biomasses for copper biosorption. Bioresour. Technol., 99, 2008, 2559-2565.

  • HADJOUDJA, S., DELUCHAT, V., BAUDU, M.: Cell surface characterization of Microcystic aeruginosa and Chlorella vulgaris. J. Colloid Interface Sci., 342, 2010, 293-299.

  • KADUKOVÁ, J., HORVÁTHOVÁ, H.: Biosorption of copper, zinc and nickel from multi-ion solutions. Biotechnology&Metals, 2nd International Conference, Košice, 2011, 45-48.

  • MALIK, D.J., STREAT, J., GREIG, J.: Characterization and evaluation of seaweedbased sorbents for treating toxic metal-bearing solutions. Process Saf. Environ. Protect., 77, 1999, 227-233.

  • MURPHY, V., HUGHES, H., MCLOUGHLIN, P.: Cu(II) binding dried biomass of red, green and brown macroalgae. Water Res., 41, 2007, 731-740.

  • MURPHY, V., HUGHES, H., MCLOUGHLIN, P.: Enhancement strategies for Cu(II), Cr(III) and Cr(VI) remediation by variety of seaweed species. J. Hazard. Mater., 166, 2009, 318-326.

  • NAJA, G., MUSTIN, CH., VOLESKY, B., BERTHELIN, J.: A high-resolution titrator: a new approach to studying binding sites of microbial biosorbents. Water Res., 39, 2005, 579-588.

  • ROMERA, E., GONZÁLEZ, F., BALLESTER, A., BLÁZQUEZ, M.L., MUÑOZ, J.A.: Comparative study of biosorption of heavy metals using different types of algae. Bioresour. Technol., 98, 2007, 3344-3353.

  • ROSSOTTI, F.J.C., ROSSOTTI, H.: Potentiometric titrations using Gran plots. J. Chem. Educ., 42, No. 7, 1965, 375-378.

  • SCHIEWER, S., WONG, M.H.: Ionic strength effects in biosorption of metals by marine algae. Chemosphere, 41, 2000, 271-282.

  • VOLESKY, B.: Sorption and biosorption. BV Sorbex, Inc., Montreal, 2003, 316 pp.

  • WANG, J., CHEN, C.: Biosorbents for heavy metals removal and their future. Biotechnol. Adv., 27, 2009, 195-226.

OPEN ACCESS

Journal + Issues

Search