Comparison of Paraffin and Diesel Oil as Cultivation Medium Supplements for Preparing a Hydrocarbon-Degrading Bacterial Biomass

Open access

Abstract

The effect of liquid paraffin and diesel oil as nutrient amendments for hydrocarbon-degrading bacteria was compared. Different parameters were analyzed - optical density of bacterial suspension, oxygen consumption by biomass, morphology of bacteria, etc. In some experiments the paraffin was more preferable for microorganisms, but in other tests the results for both substances were similar. The influence of the comparable substances strongly depends on cultivation conditions.

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • [1] Y. Pi L. Meng M. Bao P. Sun J. Lu “Degradation of crude oil and relationship with bacteria and enzymatic activities in laboratory testing” International Biodeterioration and Biodegradation vol. 106 pp. 106-116 2016. http://dx.doi.org/10.1016/j.ibiod.2015.10.015

  • [2] D. Vecstaudža O. Muter M. Grūbe J. Truu U. Kalnenieks “Optimisation of growth of Pseudomonas and Stenotrophomonas genus cultures in the presence of hydrocarbons” in: Abstract of the 72nd Scientific Conference of the University of Latvia. Environmental and Experimental Biology vol. 12 p. 67 2014.

  • [3] U. Kalnenieks M. Grube D. Vecstaudza R. Rutkis J. Truu O. Muter K.Stankevica “Improvement of hydrocarbons-degrading bacteria consortium via directed evolution” European Biotechnology Congress 2014 Journal of Biotechnology vol. 185 Supplement September 2014 S60. http://dx.doi.org/10.1016/j.jbiotec.2014.07.203

  • [4] A. Wentzel T.E. Ellingsen H.-K. Kotlar S.B. Zotchev M. Throne- Holst “Bacterial metabolism of long-chain n-alkanes” Applied Microbiology and Biotechnology vol. 76 no. 6 pp. 1209-1221 2007. http://dx.doi.org/10.1007/s00253-007-1119-1

  • [5] O. Acer K. Güven F.M. Bekler R. Gül-Güven “Isolation and characterization of long-chain alkane-degrading Acinetobacter sp. BT1A from oil-contaminated soil in Diyarbakir in the Southeast of Turkey” Bioremediation Journal vol. 20 no. 1 pp. 80-87 2016. http://dx.doi.org/10.1080/10889868.2015.1096898

  • [6] J. Zampolli E. Collina M. Lasagni P. Di Gennaro “Biodegradation of variable-chain-lengthn-alkanes in Rhodococcus opacus R7 and the involvement of an alkane hydroxylase system in the metabolism” AMB Express vol. 4 p. 73 2014. http://dx.doi.org/10.1186/s13568-014-0073-4

  • [7] G. Lan Q. Fan Y. Liu Y. Liu Y. Liu X. Yin M. Luo “Effects of the addition of waste cooking oil on heavy crude oil biodegradation and microbial enhanced oil recovery using Pseudomonas sp. SWP-4” Biochemical Engineering Journal vol. 103 pp. 219-226 2015. http://dx.doi.org/10.1016/j.bej.2015.08.004

  • [8] K. Laczi A. Kis B. Horváth G. Maróti B. Hegedüs K. Perei G. Rákhely “Metabolic responses of Rhodococcus erythropolis PR4 grown on diesel oil and various hydrocarbon” Applied Microbiology and Biotechnology vol. 99(22) pp. 9745-9759 2015. http://dx.doi.org/10.1007/s00253-015-6936-z

  • [9] A. Laškovs E. Laškovs A. Grinbergs A. Uljanovs O. Mutere „Consortium of microorganisms-destructors P1035 and uses thereof for oil product degradation” Latvian patent LV 14347 Patenti un preču zīmes vol.5 pp. 647-648 2011.

  • [10] O. Muter N. Galinina U. Kalnenieks “Monitoring of biomass growth in turbid media: optical density versus ATP concentration for oil degrading bacteria” in: Soil ecosystem health and management of contaminated sites Ed. by J.Truu and U.Kalnenieks Proceedings of Congress of Baltic Microbiologists ISBN 978-9949-32-931-1 (pdf) University of Tartu Press pp. 9-21 2014.

  • [11] R. Mailloux J. Lemire V. D. Appanna “Metabolic networks to combat oxidative stress in Pseudomonas fluorescens. Antonie van Leeuwenhoek” Int. J. Gen. Mol. Microbiol. vol. 99(3) pp. 433-442 2011. http://dx.doi.org/10.1007/s10482-010-9538-x

  • [12] O. Muter M. Grube U. Kalnenieks “An increased sensitivity of hydrocarbons-degrading bacteria to heavy metals in the presence of diesel oil’ Journal of Biotechnology vol. 208 Suppl. 20 August 2015 p. S52. http://dx.doi.org/10.1016/j.jbiotec.2015.06.153

Search
Journal information
Metrics
All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 376 250 6
PDF Downloads 122 88 0