Examination of Sol-Gel Derived Hydroxyapatite Enhanced with Silver Nanoparticles using OCT and Raman Spectroscopy

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Abstract

Hydroxyapatite (HAp) has been attracting widespread interest in medical applications. In a form of coating, it enables to create a durable bond between an implant and surrounding bone tissues. With addition of silver nanoparticles HAp should also provide antibacterial activity. The aim of this research was to evaluate the composition of hydroxyapatite with silver nanoparticles in a non-destructive and non-contact way. For control measurements of HAp molecular composition and solvent evaporation efficiency the Raman spectroscopy has been chosen. In order to evaluate dispersion and concentration of the silver nanoparticles inside the hydroxyapatite matrix, the optical coherence tomography (OCT) has been used. Five samples were developed and examined ‒ a reference sample of pure HAp sol and four samples of HAp colloids with different silver nanoparticle solution volume ratios. The Raman spectra for each solution have been obtained and analyzed. Furthermore, a transverse-sectional visualization of every sample has been created and examined by means of OCT.

References

  • [1] Leventouri, Th. (2006). Synthetic and biological hydroxyapatites: Crystal structure questions. Biomaterials, 27, 3339−3342.

  • [2] Sadat-Shojai, M., Khorasani, M.T., Dinpanah-Khoshdargi, E., Jamshidi, A. (2013). Synthesis methods for nanosized hydroxyapatite with diverse structures. Acta Biomaterialia, 9, 7591−7621.

  • [3] Eilberg, R.G., Zuckerberg, D.A. (1975). Mineralization of Invertebrate Cartilage. Calcif. Tiss. Res., 19, 85−90.

  • [4] Orlovskii, V.P., Komlev, V.S., Barinov, S.M. (2002). Hydroxyapatite and Hydroxyapatite-Based Ceramics. Inorganic Materials, 38(10), 973–984.

  • [5] Afshar, A., Ghorbani, M., Ehsani, N., Saeri, M.R., Sorrell, C.C. (2003). Some important factors in the wet precipitation process of hydroxyapatite. Materials and Design, 24, 197−202.

  • [6] Mobasherpour, I., Soulati Heshajin, M., Kazemzadeh, A., Zakeri, M. (2007). Synthesis of nanocrystalline hydroxyapatite by using precipitation method. Journal of Alloys and Compounds, 430, 330−333.

  • [7] Bogdanoviciene, I., Beganskiene, A., Tonsuaadu, K., Glaser, J., Meyer, H.J., Kareiva, A. (2006). Calcium hydroxyapatite, Ca10(PO4)6(OH)2 ceramics prepared by aqueous sol-gel processing. Materials Research Bulletin, 41, 1754−1762.

  • [8] Pramanik, S., Agarwal, A.K., Rai, K.N., Garg, A. (2007). Development of high strength hydroxyapatite by solid-state-sintering process. Ceramics International, 33, 419−426.

  • [9] Wang, Y., Zhang, S., Wei, K., Zhao, N., Chen, J., Wang, X. (2006). Hydrothermal synthesis of hydroxyapatite nanopowders using cationic surfactant as a template. Materials Letters, 60, 1484−1487.

  • [10] Teshima, K., Lee, S., Sakurai, M., Kameno, Y., Yubuta, K., Suzuki, T., Shishido, T., Endo, M., Oishi, S. (2009). Well-Formed One-Dimensional Hydroxyapatite Crystals Grown by an Environmentally Friendly Flux Method. Crystals Growth & Design, 9(6), 2937−2940.

  • [11] Mohseni, E., Zalnezhad, E., Bushroa, A.R. (2014). Comparative investigation on the adhesion of hydroxyapatite coating on Ti-6Al-4V implant: A review paper. International Journal of Adhesion & Adhesives, 48, 238−257.

  • [12] Brinker, C.J., Scherer, G.W. (1990). Sol-Gel Science: The Physics and Chemistry of Sol-Gel Processing. USA: Academic Press.

  • [13] Uklejewski, R., Winiecki, M., Mielniczuk, J., Rogala, P., Auguściński, A. (2008). The poroaccessibility parameters for three-dimensional characterization of orthopedic implants porous coatings. Metrol. Meas. Syst., 15(2), 215−226.

  • [14] Batory, D., Gawronski, J., Kaczorowski, W., Niedzielska, A. (2012). C-HAp composite layers deposited onto AISI 316L austenitic steel. Surface & Coatings Technology, 206, 2110−2114.

  • [15] Andrade, F.A.C., de Oliveira Vercik, L.C., Monteiro, F.J., da Silva Rigo, E.C. (2016). Preparation, characterization and antibacterial properties of silver nanoparticles-hydroxyapatite composites by a simple and eco-friendly method. Ceramics International, 42, 2271−2280.

  • [16] Tian, B., Chen, W., Yu, D., Lei, Y., Ke, Q., Guo, Y., Zhu, Z. (2016). Fabrication of silver nanoparticle-doped hydroxyapatite coatings with oriented block arrays for enhancing bactericidal effect and osteoinductivity. Journal of the mechanical behavior of biomedical materials, 61, 345−359.

  • [17] Fercher, A.F., Drexler, W., Hitzenberger, C.K., Lasser, T. (2003). Optical coherence tomography - principles and applications. Reports on Progress in Physics, 66, 239−303.

  • [18] Strąkowski, M.R., Pluciński, J., Jędrzejewska-Szczerska, M., Hypszer, R., Maciejewski, M., Kosmowski, B.B. (2008). Polarization sensitive optical coherence tomography for technical materials investigation. Sensors and Actuators A, 142, 104−110.

  • [19] Trojanowski, M., Kraszewski, M., Strąkowski, M.R., Pluciński, J. (2015). Optical Coherence Tomography for nanoparticles quantitative characterization. Proc. SPIE 9554, Nanoimaging and Nanospectroscopy III, 95540I.

  • [20] Strąkowski, M.R., Pluciński, J., Kosmowski, B.B. (2011). Polarization Sensitive Optical Coherence Tomography with Spectroscopic Analysis. Acta Physica Polonica A, 120(4), 785−788.

  • [21] Pircher, M., Hitzenberger, C.K., Schmidt-Erfurth, U. (2011). Polarization sensitive optical coherence tomography in the human eye. Progress in Retinal and Eye Research, 30, 431−451.

  • [22] Kwiatkowski, A., Gnyba, M., Smulko, J., Wierzba, P. (2010). Algorithms of chemicals detection using Raman spectra. Metrol. Meas. Syst., 17(4), 549−560.

  • [23] Ferraro, J.R., Nakamoto, K., Brown, C.W. (2003). Introductory Raman Spectroscopy. Elsevier.

  • [24] Kim, I., Kumta, P.N. (2004). Sol-gel synthesis and characterization of nanostructured hydroxyapatite powder. Materials Science and Engineering B, 111, 232−236.

  • [25] Zielinska, A., Skwarek, E., Zaleska, A., Gazda, M., Hupka, J. (2009). Preparation of silver nanoparticles with controlled particle size. Procedia Chemistry, 1, 1560−1566.

  • [26] Niemelä, P., Suhonen, J. (2001). Rugged Fiber-Optic Raman Probe for Process Monitoring Applications. Applied Spectroscopy, 55(10), 1337−1340.

  • [27] Gnyba, M., Keränen, M., Maaninen, A., Suhonen, J., Jędrzejewska-Szczerska, M., Kosmowski, B.B., Wierzba, P. (2005). Raman system for on-line monitoring and optimization of hybrid polymer gelation. Opto-Electronics Review, 13(1), 9−17.

  • [28] Gnyba, M., Keraenen, M. (2003). Optical investigation of molecular structure of sophisticated materials for photonics. Proc. SPIE, 5125, 339–344.

  • [29] Koutsopoulos, S. (2002). Synthesis and characterization of hydroxyapatite crystals: A review study on the analytical methods. Journal of Biomedical Materials Research, 62(4), 600−612.

Metrology and Measurement Systems

The Journal of Committee on Metrology and Scientific Instrumentation of Polish Academy of Sciences

Journal Information


IMPACT FACTOR 2016: 1.598

CiteScore 2016: 1.58

SCImago Journal Rank (SJR) 2016: 0.460
Source Normalized Impact per Paper (SNIP) 2016: 1.228

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