Modification of the Standard 7-Day Ovsynch Protocol to Increase the Luteolytic and Synchronization Risks in Dairy Cows

Branko Atanasov 1 , Nikola Adamov 1 , Irena Celeska 1 , Ksenija Ilievska 1 , Branko Angjelovski 1 , Zvonko Trbogazov 2 , Filip Davkov 3 , Toni Dovenski 1 , Geert Opsomer 4 , and Jeffrey Stevenson 5
  • 1 Faculty of Veterinary Medicine–Skopje, Ss. Cyril and Methodius University in Skopje, Lazar Pop Trajkov 5-7, 1000 Skopje, R. of N. Macedonia
  • 2 Dairy Farm ZSK “Strumica”, , Sushica, Strumica, R. of N. Macedonia
  • 3 Dairy Farm ZK “Pelagonija”, , Bitola, R, of N. Macedonia
  • 4 Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Ghent, Belgium
  • 5 Department of Animal Sciences and Industry, Kansas State University Manhattan, KS, USA


We hypothesized that a single dose of PGF belatedly injected on day 8 after GnRH-1 in cows receiving a 7-day Ovsynch-56 protocol (GnRH – 7 days – PGF – 56h – GnRH – 16h – timed AI) will increase the proportion of cows with complete luteolysis. At day 35±3 postpartum, 70 lactating Holstein cows from one herd were scored for body condition and pre-synchronized with PGF and GnRH (3 days apart) and 7 days later submitted to an Ovsynch-56 protocol for first AI after random assignment to two treatments: (1) OV-7 (n=35) with an injection of PGF either on day 7; or (2) OV-8 (n=35) on day 8 after G1, respectively. Blood was collected before the first PGF, at day 7 and day 8 in OV-7 and OV-8, respectively, at AI and at 7 days after AI to assess progesterone concentration. Ten cows were classified as acyclic and were excluded from the analysis resulting in 60 cows (OV-8, n=27; OV-7, n=33). In total, more (P=0.01) OV-8 cows and more (P=0.04) primiparous OV-8 cows had complete luteolysis compared with their OV-7 herd mates. In addition, more (P=0.008) OV-8 cows with BCS<2.75 had complete luteolysis compared with their OV-7 herd mates, whereas no difference was observed between treatments among cows with BCS ≥2.75. In conclusion, delaying the application of PGF by 1 day reduced the percentage of primiparous cows and cows with poorer BCS having incomplete luteal regression at the time of AI.

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