Retrospective Analysis of the Bluetongue Outbreak in Serbia

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Bluetongue, a vector-born disease caused by the Bluetongue virus (BTV) and transmitted by Culicoides biting midges, is considered to be one of the most important diseases of domestic ruminants. The first outbreak of bluetongue in Serbia was reported in 2001, when BTV serotype 9 was identified in sampled materials. In 2014, outbreak of BTV-4 in Serbia caused considerable economic losses affecting sheep, cattle and goats. During this outbreak, BTV-4 was recorded in 644 outbreaks within 49 municipalities, part of 17 administrative regions. From the total number of sheep kept in areas affected by bluetongue (n=1 748 110), 2 083 cases (0.2%) were proven to be BTV-4 infected. Total of 206 infected cattle and 24 infected goats were reported during this investigation period, which represents 0.06% and 0.03% of the total number of cattle and goats kept in affected areas, respectively. The highest incidence of infected sheep, cattle and goats was recorded on the territory covered by veterinary institute of Nis. Recorded lethality in cattle, sheep and goats was 18.45% (n=38), 48.10% (n=1002) and 54.17% (n=13), respectively. The peak of the outbreak was in September and October when 94.43% of the confirmed positive cases, regardless of the species, was recorded. Monitoring of bluetongue disease in Serbia relies on active surveillance programmes aimed at: (i) identification and tracing of susceptible and potentially infected animals and (ii) detection, distribution and prevalence of insect vectors. Vaccination of sheep is planned to be implemented as a control measure against bluetongue in Serbia.

1. Verwoerd, D., Erasmus, B. J. (2004). Bluetongue. In: J. A. Coetzer, R. C. Tustin, (Ed.), Infectious diseases of livestock. 2nd ed. (pp. 1201-1220). Cape Town: Oxford University Press.

2. Maclachlan, N.J., Drew, C.P., Darpel, K.E., Worwa, G. (2009). The pathology and pathogenesis of bluetongue. J Comp Pathol. 141, 1-16. PMid:19476953

3. Schwartz-Cornil, I., Mertens, P.P., Contreras, V., Hemati, B., Pascale, F., Breard, E., Mellor, P.S., MacLachlan, N.J., Zientara, S. (2008). Bluetongue virus: virology, pathogenesis and immunity. Vet Res. 39-46. PMid:18495078

4. King, A.M.Q., Lefkowitz, E., Adams, M.J., Carstens, E.B. (2012). Family - Reoviridae. Virus Taxonomy. (pp.541-637). San Diego: Elsevier.

5. Maan, N.S., Maan, S., Belaganahalli, M.N., Ostlund, E.N., Johnson, D.J., Nomikou, K., Mertens, P.P. (2012). Identification and differentiation of the twenty six bluetongue virus serotypes by RT-PCR amplification of the serotype-specific genome segment 2. PloS One, 7(2), pp.e32601-e32601. PMid:22389711 PMCid:PMC3289656

6. Gibbs, E.P., Greiner, E.C. (1994). The epidemiology of bluetongue. Comp Immunol Microb Infect Dis. 17, 197-206.

7. Tabachnick, W.J. (2004). Culicoides and the global epidemiology of bluetongue virus infection. Vet Ital. 40, 145-150.

8. Mellor, P.S., Boorman, J. (1995). The transmission and geographical spread of African horse sickness and bluetongue viruses. Ann Trop Med Parasit. 89, 1-15. PMid:7741589

9. Darpel, K.E., Batten, C.A., Veronesi, E., Shaw, A.E., Anthony, S., Bachanek-Bankowska, K., Kgosana, L., Bin-Tarif, A., Carpenter, S., Muller-Doblies, U.U., Takamatsu, H.H., Mellor, P.S., Mertens, P.P.C. (2007). Clinical signs and pathology shown by British sheep and cattle infected with bluetongue virus serotype 8 derived from the 2006 outbreak in northern Europe. Vet Rec. 161, 253-261. PMid:17720961

10. Mellor, P.S., Carpenter, S., Harrup, L., Baylis, M., Mertens, P.P. (2008). Bluetongue in Europe and the Mediterranean Basin: history of occurrence prior to 2006. Prev Vet Med. 87, 4-20. PMid:18619694

11. Maclachlan, N.J. (2011). Bluetongue: history, global epidemiology, and pathogenesis. Prev Vet Med. 102, 107-111. PMid:21570141

12. Barratt-Boyes, S.M., MacLachlan, N.J. (1995). Pathogenesis of bluetonguevirus infection of cattle. J Am Vet Med A. 206, 1322-1329. PMid:7775242

13. OIE Disease Information. [Internet]. Bluetongue.

14. Sperlova, A., Zendulkova, D. (2011). Bluetongue: a review. Vet Med. 56, 430-452.

15. Anon. (2001a). Bluetongue in Greece and Italy. Off Int Epizoot Dis Info. 14, 215-218.

16. Wilson, A.J., Mellor, P.S. (2009). Bluetongue in Europe: past, present and future. Philos T Roy Soc B. 364, 2669-2681. PMid:19687037 PMCid:PMC2865089

17. Saegerman, C., Berkvens, D., Mellor, P.S. (2008). Bluetongue epidemiology in the European union. Emerg Infect Dis. 14, 539-544. PMid:18394269 PMCid:PMC2570923

18. Rodríguez-Sánchez, B., Iglesias-Martín, I., Martínez-Avilés, M., Sánchez-Vizcaíno, J.M. (2008). Orbiviruses in the Mediterranean Basin: updated epidemiologicalsituation of Bluetongue and new methods for the detection of BTV serotype 4. Transbound Emerg Dis. 55, 205-214. PMid:18666964

19. Zientara, S., MacLachlan, N.J., Calistri, P., Sanchez- Vizcaino, J.M., Savini, G. (2010). Bluetongue vaccination in Europe. Expert Rev Vaccines. 9, 989-991. PMid:20822340

20. De Clercq, K., Mertens, P., De Leeuw, I., Oura, C., Houdart, P., Potgieter, A.C., Maan, S., et al. (2009). Emergence of bluetongue serotypes in Europe. Part 2. The occurrence of a BTV-11 strain in Belgium. Transbound Emerg Dis. 9/10, 355-361. PMid:19909474

21. Eschbaumer, M., Hoffmann, B., Moss, A., Savini, G., Leone, A., Konig, P., Zemke, J., Conraths, F., Beer, M. (2010). Emergence of bluetongue virus serotype 6 in Europe-German field data and experimental infection in cattle. Vet Microbiol. 143, 189-195. PMid:20045271

22. Ganter, M. (2014). Bluetongue disease-Global overview and future risks. Small Ruminant Res. 118, 79-85.

23. Djuricic, B., Nedic, D., Lausevic, D., Pavlovic, M. (2004). The epizootiological occurrence of bluetongue in the central Balkans. Vet Ital. 40, 105-107. PMid:20419644

24. Anon. (2001b). Bluetongue in the Former Yugoslav Republic of Macedonia. Off Int Epizoot Dis Info. 14, 234.

25. Mellor, P.S., Wittmann, E.J. (2002). Bluetongue virus in the Mediterranean basin 1998-2001. Vet J. 164, 20-37. PMid:12359482

26. Zientara, S., Sánchez-Vizcaíno, J.M. (2013). Control of bluetongue in Europe. Vet Microbiol. 165, 33-37. PMid:23462519

27. OIE - World Organisation for Animal Health [Internet]. 72nd General Session, 23-28 May 2004.

28. OIE - World Organisation for Animal Health [Internet]. Manual of diagnostic tests and vaccines for terrestrial animals. Chapter 2.01.03. Bluetongue, 2014.

29. Hofmann, M., Griot, C., Chaignat, V., Perler, L., Thür, B. (2008). Bluetongue disease reaches Switzerland. Schweiz Arch Tierheilk, 150, 49-56. PMid:18369049

30. Anon. (1998a). Bluetongue in Greece: confirmation of diagnosis. Dis Info. 11, 166-167.

31. Anon. (1998b). Disease outbreaks reported to the OIE in November-December. Bull Off Int Epizoot. 110, 506.

32. Taylor, W.P., Mellor, P.S. (1994). Distribution of bluetongue virus in Turkey, 1978-81. Epidemiol Infect. 112, 623-633. PMid:8005228 PMCid:PMC2271514

33. Labrovic, A., Poljak, Z., Separovic, S., Jukic, B., Lukman, D., Listes, E., Bosnic, S. (2004). Spatial distribution of bluetongue in cattle in southern Croatia in the last quarter of 2002. Vet Ital. 40, 217-220. PMid:20419667

34. Listes, E., Bosnic, S., Beni, M., Lojkic, M., Cac, Z., Cvetnic, Z., Madic, J., Separovic, S., Labrovic, A., Savini, G., Goffredo, M. (2004). Serological evidence of bluetongue and a preliminary entomological study in southern Croatia. Vet Ital. 40, 221-225. PMid:20419668

35. OIE - World Organisation for Animal Health [Internet]. Bluetongue, Serbia, 2015. [Accessed 30 October 2015.]

36. OIE - World Organisation for Animal Health [Internet]. Weekly Disease Information, 2015b, [Accessed 30 October 2015].

37. Roberts, H., Smith, J., Batten, C., Nomikou, K., Mertens, P. (2014). Preliminary outbreak assessment - Bluetongue virus (BTV-4) in Greece, Bulgaria and the Republic of Macedonia.

38. Mehlhorn, H., Walldorf, V., Klimpel, S., Schaub, G., Kiel, E., Focke, R., Liebisch, G., Liebisch, A., Werner, D., Bauer, C., Clausen, H., Bauer, B., Geier, M., Hörbrand, T., Bätza, H.J., Conraths, F.J., Hoffmann, B., Beer, M. (2009). Bluetongue disease in Germany (2007-2008): monitoring of entomological aspects. Parasitol Res. 105, 313-319. PMid:19322587

39. Purse, BV., Mellor, P.S., Rogers, D.J., Samuel, A.R., Mertens, P.P.C., Baylis, M. (2005). Climate change and the recent emergence of bluetongue in Europe. Nat Rev Microbiol. 3, 171-181. PMid:15685226

Macedonian Veterinary Review

The Journal of the Faculty of Veterinary Medicine-Skopje at the Ss. Cyril and Methodius University in Skopje

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CiteScore 2016: 0.28

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