Biobutanol Production from Agricultural Waste: A Simple Approach for Pre-Treatment and Hydrolysis

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Abstract

One of the main concerns regarding extensive production of biobutanol has been associated with the high costs of the substrate (preparation of fermentable sugars) and the relatively low tolerance of Clostridia to butanol. In this study a simple, mild approach was tested to obtain fermentable sugars from agricultural waste. Giant hogweed and hay was pre-treated with simple boiling and enzymatically hydrolysed. The results demonstrated that after adaptation of the genus Clostridium bacteria to the new substrate, the growth kinetics and sugar consumption of these bacteria were similar to the ones obtained in traditional culture media.

Abstract

Samazinoties pasaules naftas rezervēm, arvien lielāka uzmanība tiek pievērsta alternatīvās enerģijas ieguves veidiem. Viena no alternatīvām degvielām var būt biobutanols, ko fermentācijas laikā dabiski veido klostrīdiju ģints baktērijas. Līdz šim par vienu no kavējošiem aspektiem biobutanola plašākai lietošanai tika uzskatītas salīdzinoši dārgās ražošanas un substrāta izmaksas. Pētījumā parādīts, kā iespējams iegūt biobutanolu, izmantojot pārtikai nepiemēroto biomasu (siens, latvāņi). Procesa vienkāršošanai un izmaksu samazināšanai tradicionālās ķīmiskās un augstspiediena priekšapstrādes un hidrolīzes tehnoloģijas tika aizvietotas ar vienkāršu substrāta vārīšanu un enzimātisko hidrolīzi. Konstatēts, ka pēc nelielas klostrīdiju adaptēšanas jaunajos vides apstākļos klasiskajā augšanas barotnē un vidē ar pievienotu hidrolizātu būtiski neatšķīrās šūnu augšana un fermentējamo cukuru patēriņš. Tādējādi secināts, ka optimizācijas procesā iespējams samazināt biobutanola iegūšanas izmaksas un vienkāršot tehnoloģiskos procesu.

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Latvian Journal of Chemistry

The Journal of Riga Technical University

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