The objective of this work is firstly an ecobiological study, which aims to determine whether salinity is favorable for a good reproduction rate of cysts in the Artemia salina L., a species known for its use in aquaculture. Secondly, a short-term test of ammonium sulfate toxicity on its vitality is carried out as its environment is permanently subjected to sulphate-laden waste. The work consists of subjecting the species to different salinities in order to produce a good yield in cysts, which once recovered, hatching and breeding is maintained until individuals reach the adult stage. A toxicological study using toxicity test (CL50) is then conducted to study the resistance of these adults with various concentrations of ammonium sulphate. The results obtained from the ecobiological study show a mean similar to the natural environment (p > 0.05) with a favorable concentration for the reproduction of the species at a salinity of about 75 g dm−3. The toxicity test indicates a CL50 equal to 75 mg dm−3 after 24 hours. It is obvious that Artemia salina L. can be cultivated by controlling its reproduction by varying the salinity rate for its use during different seasons of the year, thus ammonium sulphate from waste is considered as a limiting factor.
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Abatzopoulos T.J. Beardmore J.A. Clegg JS.. Sorgeloos P. (eds) 2002 Artemia. Basic and applied biology Kluwer Academic Publishers Dordrecht 286 pp.
Abu Shaala N. Zulkifli S. Ismail A. Azmai M. Yusuff M. 2015 Lethal concentration 50 (LC50) and effects of Diuron on morphologyof brine shrimp Artemia salina (Branchiopoda: Anostraca) Nauplii Procedia Environ. Sci. 30: 279–284.
Bennabi F. Benmeddah M. Toumi F. Hamel L. Megharbi A. Koudache F. Ghomari S. 2015 Etude éco-biologique d’Artémia salina des zones humides de l’Ouest Algérien (Eco-biological study of Artemia salina L. of West Algeria) Afrique Science 11(2): 97–106 (in French English summary).
Browne R.A. Wanigasekera G. 2000 Combined effects of salinity and temperature on survival and reproduction of five species of Artemia J. Exp. Mar. Bio. Ecol. 244: 29–44.
Calbet A. Garrido S. Saiz E. Alcaraz M. Duarte C.M. 2001 Annual zooplankton succession in coastal NW Mediterranean waters: the importance of the smaller fractions J.Plankton Res. 23(3): 319–331.
Delisle C.E. Schmidt J.W. 1977 The effect of sulfur and water life in Canada [in:] Cooke N.E. (ed.) Sulfur and its inorganic derivatives in the Canadian environment Publ. No. NRCC 15015 Natn. Res. Coun. Can. Ottawa 426 pp.
Derwich E. Benaabidate L. Zian A. Sadki O. Belghity D. 2010 Caracterisation physico-chimique des eaux de la nappe alluviale du haut Sebou en aval de sa confluence avec Oued Fès (Physico-chemical characterization of the waters of the Upper Sebou alluvial aquifer downstream from its confluence with Oued Fès) Larhyss J. 8: 101–112 (in French).
Greenwood N.N. Earnshaw A. 1984 Chemistry of the Elements Pergamon Press Oxford 1542 pp.
Hoffmeyer M.S. 1994 Seasonal succession of Copepoda in the Bah í a Blanca estuary Hydrobiologia 292(1): 303–308.
IBM Corp. Released 2015 IBM SPSS Statistics for Windows (Version 23.0) [Computer software] IBM Corp. Armonk.
Kherraz-Chemlal D. Boukhatem T. Khelil F. Sahnouni F. Maatalah A. Boutiba Z. 2017 Determination of biological characteristics of Artemia salina (Crustacea: Anostraca) population from saline Bethioua (Oran Algeria) Int. J. Biosci. 10(1): 117–125.
Lavens P. Sorgeloos P. 2000 The history present status and prospect of the availability of Artemia cysts for aquaculture Aquaculture 181: 397–403.
Lei Y. Xu K. Ki Choi J. Pyo Hong H. Wickham S.A. 2009 The structure and seasonal dynamics of planktonic ciliates along salinity gradients Eur. J. Protistol. 45: 305–319.
Libralato G. Prato E. Migliore L. Cicero A.M. Manfra A. 2016 Review of toxicity testing and endpoints with Artemia spp. Ecol. Indic. 69: 35–49.
Manfra L. Tornambè A. Savorelli F. Rotini A. Canepa S. Mannozzi M. Cicero A.M. 2015a Ecotoxicity of diethylene glycol and risk assessment for marine environment J. Hazard. Mater. 284: 130–135.
Manfra L. Savorelli F. Di Lorenzo B. Libralato G. Comin S. Conti D. Floris B. Francese M. Gallo M. L. Gartner I. Guida M. Leoni T. Marino G. Martelli F. Palazzi D. Prato E. Righini P. Rossi E. VolpiGhirardini A. Migliore L. 2015b Intercalibration of ecotoxicity testing protocols with Artemia franciscana Ecol. Indic. 57: 41–47.
[OECD] Organization for Economic Cooperation and Development 1992 OECD Guideline for testing of chemicals. Test No. 203: Fish acute toxicity test OECD Publishing Paris 9 pp.
Parekh H.M Tank S.K. 2015 Studies of haematological parameters of Oreochromis niloticus exposed to cadmium chloride (CdCl2 2H2O) Int. J. Environ. 4(2): 116–127.
Patel J.G. Nirmal Kumar J.I. Kumar R.N. Shamiyan R.K. 2013 Chronic toxicity of high molecular weight polynuclear aromatic hydrocarbonpyrene on freshwater cyanobacterium anabaena fertlissimarao Int. J. Environ. 2(1): 175–183.
Person-Le Ruyet J. 1976 Techniques d’élevage en masse d’un rotifère (Brachionus plicatilis Müller) et d’un crustacé branchiopode (Artemia salina L) (Techniques of mass culture of the rotifer (Brachionus plicatilis Müller) and a branchiopod crustacean (Artemia salina L.) [in:] Persoone G. Jaspers E. (eds) Proc. of the10th European Symposium on Marine Biology. Vol. 1: Research in mariculture at laboratory- and pilot scale 17–23 September 1975 Ostend: 331–343 (in French English summary).
Sorgeloos P. Dhert P. Candreva P. 2001 Use of the brine shrimp Artemia spp. in marine larviculture Aquaculture 200(1–2): 147–159.
Triantaphyllidis G.V. Poulopoulou K. Abatzpoulos T.J. Perez C.A. Sorgeloos P. 1995 International study on Artemia XLIX. Salinity effects on survival maturity growth biometrics reproductive and lifespan characteristics of a bisexual and parthenogenetic population of Artemia Hydrobiology 302: 215–227.
Trigui K. Bensaid R. Akrout F. Aloui N. 2016 Etude éco-biologique d’Artemia salina (Branchiopoda Anostraca) de la saline de Sahline (Tunisie) (Eco-biological study of Artemia salina L. Branchiopoda Anostraca in Sahline Salt Lake (Tunisia) Crustaceana 89(8): 949–974.
Vieira N. Bio A. 2011 Spatial and temporal variability of water quality and zooplankton in an artisanal salina J. Sea Res. 65(2): 293–303.