The effect of glyphosate-based herbicide on aquatic organisms – a case study

Piotr Rzymski 1 , Piotr Klimaszyk 2 , Tomasz Kubacki 3 , and Barbara Poniedziałek 3
  • 1 Department of Biology and Environmental Protection, Poznan University of Medical Sciences, Rokietnicka 8, 60-806 Poznań, Poland,
  • 2 Department of Water Protection, Adam Mickiewicz University, Umultowska 89, 61-614 Poznań, Poland
  • 3 Department of Biology and Environmental Protection, Poznan University of Medical Sciences, Rokietnicka 8, 60-806 Poznań, Poland


The non-selective, post-emergence herbicides based on glyphosate [N-(phosphonomethyl) glycine] are one of the most widely used pesticides in agriculture, urban areas and forestry. Although there has been documentation on the physical, chemical and toxicological properties of glyphosate, the aquatic toxicity of such formulations still requires assessment and evaluation. In the present study, we describe deliberate use of glyphosate-based herbicide in a bathing area of Lake Lednica (Wielkopolska, Poland) by unknown perpetrators in April, 2011. Glyphosate was detected using gas chromatography mass spectrometry (GC-MS) in the water samples collected from the bathing area at a mean concentration of 0.09 mg dm-3. Aboveground parts of emerged macrophytes (Phragmites australis and Typha latifolia) covering the investigated area were completely withered. Studies of benthic macroinvertebrates revealed no significant differences in taxa number between event (13 taxa) and control (14 taxa) sites although differences in abundance of particular taxa were observed. Significantly lower numbers of Chironomidae (by 41%), Oligochaeta (by 43%), Vivipariae (by 75%), Hirudinae (by 75%), Asellus aquaticus (by 77%), Gammarus pulex (by 38%) and Dreissena polymorpha (by 42%) were found at the glyphosate-treated site. Furthermore, compared to the control, chironomids (Chironomidae) exposed to glyphosate were represented by specimens smaller in length while A. aquaticus only showed large adults. The ranges of glyphosate concentration in the tissues of sampled macroinvertebrates and Phragmites australis organs were 7.3-10.2 μg kg-1 and 16.2-24.7 μg kg-1, respectively. Our study indicates that glyphosate-based herbicides may have adverse effects on aquatic organisms including macroinvertebrates, thus their use in (or nearby) surface waters should be subject to strict limitation.

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  • Bonnet J.L., Bonnemoy F., Dusser M., Bohatier J., 2007, Assessment of the potential toxicity of herbicides and their degradation products to non target cells using two microorganisms, the bacteria Vibriof ischeri and the ciliate Tetrahymena pyriformis, Environ. Toxicol. 22(1): 78-91.

  • Buhl K.J., Faerber N.L., 1989, Acute toxicity of selected herbicides and surfactants to larvae of the midge Chironomus riparius, Arch. Environ. Contam. Toxicol. 18(4): 530-536.

  • Contardo-Jara V., Klingelmann E., Wiegand C., 2009, Bioaccumulation of glyphosate and its formulation Roundup Ultra in Lumbriculus variegatus and its effects on biotransformation and antioxidant enzymes, Environ. Pollut. 157(1): 57-63.

  • Coupe R.H., Kalkhoff S.J., Capel P.D., Gregoire C., 2012, Fate and transport of glyphosate and aminomethylphosphonic acid in surface waters of agricultural basins, Pest. Manag. Sci. 68(1): 16-30.

  • Edge C.B., Gahl M.K., Thompson D.G., Houlahan J.E., 2013, Laboratory and field exposure of two species of juvenile amphibians to a glyphosate-based herbicide and Batrachochytrium dendrobatidis, Sci. Total Environ. 444: 145-152.

  • Folmar L.C., Sanders H.L., Julin A.M., 1979, Toxicity of the herbicide glyphosate and several of its formulations to fish and aquatic invertebrates, Arch. Environ. Contam. Toxicol. 8(3): 269-278.

  • Giesy J.P., Dobson S., Solomon K.R., 2000, Ecotoxicological risk assessment for Roundup herbicide, Rev. Environ. Contam. Toxicol. 167: 35-120.

  • Glusczak L., Loro V.L., Pretto A., Moraes B.S., Raabe A., Duarte M.F., da Fonseca M.B., de Menezes C.C., Valladao D.M., 2011, Acute exposure to glyphosate herbicide affects oxidative parameters in piava (Leporinus obtusidens), Arch. Environ. Contam. Toxicol. 61(4): 624-630.

  • Goldsborough L.G., Beck A.E., 1989, Rapid dissipation of glyphosate in small forest ponds, Arch. Environ. Contam. Toxicol. 18(4): 537-544.

  • Jones D.K., Hammond J.I., Relyea R.A., 2010, Roundup and amphibians: the importance of concentration, application time, and stratification, Environ. Toxicol. Chem. 29(9): 2016-2025.

  • Klimaszyk P., Heymann D., 2010, Vertical distribution of benthic macroinvertebrates in a meromictic lake (Lake Czarne, Drawieński National Park), Oceanol. Hydrobiol. Stud. 39(7): 99-106.

  • Linz G.M., Bleier W.J., Overland J.D., Homan H.J., 1999, Response of invertebrates to glyphosate-induced habitat alterations in wetlands, Wetlands 19(1): 220-227.

  • Linz G.M., Homar H.J., 2011, Use of glyphosate for managing invasive cattail (Typha spp.) to disperse blackbird (Icteridae) roosts, Crop Protect. 30(2): 98-104.

  • Modesto K.A., Martinez C.B., 2010, Effects of Roundup Transorb on fish: hematology, antioxidant defenses and acetylcholinesterase activity, Chemosphere 81(6): 781-787.

  • Perez G.P., Vera M.S., Miranda L., 2011, Effects of Herbicide Glyphosate and Glyphosate-Based Formulations on Aquatic Ecosystems, [in:] Kortekamp A. (ed.), Herbicides and Environment, InTech Europe, Rijeka: 343-368.

  • Peruzzo P.J., Porta A.A., Ronco A.E., 2008, Levels of glyphosate in surface waters, sediments and soils associated with direct sowing soybean cultivation in north pampasic region of Argentina, Environ. Pollut. 156(1): 61-66.

  • Pieniążek D., Bukowska B., Duda W., 2003, Glifosat - nietoksyczny pestycyd? (Glyphosate - A non-toxic pesticide?) Med. Pracy 54(6): 579-583 (in Polish, English summary).

  • Relyea R. A., 2005, The impact of insecticides and herbicides on the biodiversity and productivity of aquatic communities, Ecol. Appl. 15(2): 618-627.

  • Rueppel M.L., Brightwell B.B., Schaefer J., Marvel J.T., 1977, Metabolism and degradation of glyphosate in soil and water, J. Agric. Food Chem. 25(3): 517-528.

  • Sandrini J.Z., Rola R.C., Lopes F.M., Buffon H.F., Freitas M.M., Martins Cde M., da Rosa C.E., 2013, Effects of glyphosate on cholinesterase activity of the mussel Perna perna and the fish Danio rerio and Jenynsia multidentata: In vitro studies, Aquat. Toxicol. 130-131: 171-173.

  • Schmidt H., Boas P., 2006, Accompanying experiments on weed control on public footways using the roller wiper ‘Rotofix’, Nachrichtenbl. Deut. Pflanzenschutzd. 58(2): 46-49.

  • Solomon K.R., Thompson D.G., 2003, Ecological risk assessment for aquatic organisms from over-water uses of glyphosate, J. Toxicol. Environ. Health B Crit. Rev. 6(3): 289-324.

  • Steinrucken H.C., Amrhein N., 1980, The herbicide glyphosate is a potent inhibitor of 5-enolpyruvylshikimic acid-3-phosphate synthase, Biochem. Biophys. Res. Comm. 94(4): 1207-1212.

  • Tsui M.T.K., Chu L.M., 2004, Comparative toxicity of glyphosate- based herbicides: aqueous and sediment porewater exposures, Arch. Environ. Contam. Toxicol. 46(3): 316-323. [USEPA] U.S. Environmental Protection Agency, 1993, R.E- .D. Facts: glyphosate, US EPA: Office of Pesticide Programs, Washington, p. 7. [USEPA]

  • U.S. Environmental Protection Agency, 1986, Guidance for the reregistration of pesticide products containing glyphosate as the active ingredient, US EPA: Office of Pesticide Programs, Washington, p. 207.

  • Vera-Candioti J., Soloneski S., Larramendy M.L., 2013, Evaluation of the genotoxic and cytotoxic effects of glyphosate- based herbicides in the ten spotted live-bearer fish Cnesterodon decemmaculatus (Jenyns, 1842), Ecotoxicol. Environ. Saf. 89: 166-173.

  • Wang Y.S., Jaw C.G., Chen Y.L., 1994, Accumulation of 2,4- D and glyphosate in fish and water hyacinth, Water Air Soil Pollut. 74(3-4): 397-403. [WHO] World Health Organization, 1994, Glyphosate. Environmental health criteria 159, WHO: IPCS, Geneva, p. 177.


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