The effect of glyphosate-based herbicide on aquatic organisms – a case study

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Abstract

The non-selective, post-emergence herbicides based on glyphosate [N-(phosphonomethyl) glycine] are one of the most widely used pesticides in agriculture, urban areas and forestry. Although there has been documentation on the physical, chemical and toxicological properties of glyphosate, the aquatic toxicity of such formulations still requires assessment and evaluation. In the present study, we describe deliberate use of glyphosate-based herbicide in a bathing area of Lake Lednica (Wielkopolska, Poland) by unknown perpetrators in April, 2011. Glyphosate was detected using gas chromatography mass spectrometry (GC-MS) in the water samples collected from the bathing area at a mean concentration of 0.09 mg dm-3. Aboveground parts of emerged macrophytes (Phragmites australis and Typha latifolia) covering the investigated area were completely withered. Studies of benthic macroinvertebrates revealed no significant differences in taxa number between event (13 taxa) and control (14 taxa) sites although differences in abundance of particular taxa were observed. Significantly lower numbers of Chironomidae (by 41%), Oligochaeta (by 43%), Vivipariae (by 75%), Hirudinae (by 75%), Asellus aquaticus (by 77%), Gammarus pulex (by 38%) and Dreissena polymorpha (by 42%) were found at the glyphosate-treated site. Furthermore, compared to the control, chironomids (Chironomidae) exposed to glyphosate were represented by specimens smaller in length while A. aquaticus only showed large adults. The ranges of glyphosate concentration in the tissues of sampled macroinvertebrates and Phragmites australis organs were 7.3-10.2 μg kg-1 and 16.2-24.7 μg kg-1, respectively. Our study indicates that glyphosate-based herbicides may have adverse effects on aquatic organisms including macroinvertebrates, thus their use in (or nearby) surface waters should be subject to strict limitation.

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  • Bonnet J.L. Bonnemoy F. Dusser M. Bohatier J. 2007 Assessment of the potential toxicity of herbicides and their degradation products to non target cells using two microorganisms the bacteria Vibriof ischeri and the ciliate Tetrahymena pyriformis Environ. Toxicol. 22(1): 78-91.

  • Buhl K.J. Faerber N.L. 1989 Acute toxicity of selected herbicides and surfactants to larvae of the midge Chironomus riparius Arch. Environ. Contam. Toxicol. 18(4): 530-536.

  • Contardo-Jara V. Klingelmann E. Wiegand C. 2009 Bioaccumulation of glyphosate and its formulation Roundup Ultra in Lumbriculus variegatus and its effects on biotransformation and antioxidant enzymes Environ. Pollut. 157(1): 57-63.

  • Coupe R.H. Kalkhoff S.J. Capel P.D. Gregoire C. 2012 Fate and transport of glyphosate and aminomethylphosphonic acid in surface waters of agricultural basins Pest. Manag. Sci. 68(1): 16-30.

  • Edge C.B. Gahl M.K. Thompson D.G. Houlahan J.E. 2013 Laboratory and field exposure of two species of juvenile amphibians to a glyphosate-based herbicide and Batrachochytrium dendrobatidis Sci. Total Environ. 444: 145-152.

  • Folmar L.C. Sanders H.L. Julin A.M. 1979 Toxicity of the herbicide glyphosate and several of its formulations to fish and aquatic invertebrates Arch. Environ. Contam. Toxicol. 8(3): 269-278.

  • Giesy J.P. Dobson S. Solomon K.R. 2000 Ecotoxicological risk assessment for Roundup herbicide Rev. Environ. Contam. Toxicol. 167: 35-120.

  • Glusczak L. Loro V.L. Pretto A. Moraes B.S. Raabe A. Duarte M.F. da Fonseca M.B. de Menezes C.C. Valladao D.M. 2011 Acute exposure to glyphosate herbicide affects oxidative parameters in piava (Leporinus obtusidens) Arch. Environ. Contam. Toxicol. 61(4): 624-630.

  • Goldsborough L.G. Beck A.E. 1989 Rapid dissipation of glyphosate in small forest ponds Arch. Environ. Contam. Toxicol. 18(4): 537-544.

  • Jones D.K. Hammond J.I. Relyea R.A. 2010 Roundup and amphibians: the importance of concentration application time and stratification Environ. Toxicol. Chem. 29(9): 2016-2025.

  • Klimaszyk P. Heymann D. 2010 Vertical distribution of benthic macroinvertebrates in a meromictic lake (Lake Czarne Drawieński National Park) Oceanol. Hydrobiol. Stud. 39(7): 99-106.

  • Linz G.M. Bleier W.J. Overland J.D. Homan H.J. 1999 Response of invertebrates to glyphosate-induced habitat alterations in wetlands Wetlands 19(1): 220-227.

  • Linz G.M. Homar H.J. 2011 Use of glyphosate for managing invasive cattail (Typha spp.) to disperse blackbird (Icteridae) roosts Crop Protect. 30(2): 98-104.

  • Modesto K.A. Martinez C.B. 2010 Effects of Roundup Transorb on fish: hematology antioxidant defenses and acetylcholinesterase activity Chemosphere 81(6): 781-787.

  • Perez G.P. Vera M.S. Miranda L. 2011 Effects of Herbicide Glyphosate and Glyphosate-Based Formulations on Aquatic Ecosystems [in:] Kortekamp A. (ed.) Herbicides and Environment InTech Europe Rijeka: 343-368.

  • Peruzzo P.J. Porta A.A. Ronco A.E. 2008 Levels of glyphosate in surface waters sediments and soils associated with direct sowing soybean cultivation in north pampasic region of Argentina Environ. Pollut. 156(1): 61-66.

  • Pieniążek D. Bukowska B. Duda W. 2003 Glifosat - nietoksyczny pestycyd? (Glyphosate - A non-toxic pesticide?) Med. Pracy 54(6): 579-583 (in Polish English summary).

  • Relyea R. A. 2005 The impact of insecticides and herbicides on the biodiversity and productivity of aquatic communities Ecol. Appl. 15(2): 618-627.

  • Rueppel M.L. Brightwell B.B. Schaefer J. Marvel J.T. 1977 Metabolism and degradation of glyphosate in soil and water J. Agric. Food Chem. 25(3): 517-528.

  • Sandrini J.Z. Rola R.C. Lopes F.M. Buffon H.F. Freitas M.M. Martins Cde M. da Rosa C.E. 2013 Effects of glyphosate on cholinesterase activity of the mussel Perna perna and the fish Danio rerio and Jenynsia multidentata: In vitro studies Aquat. Toxicol. 130-131: 171-173.

  • Schmidt H. Boas P. 2006 Accompanying experiments on weed control on public footways using the roller wiper ‘Rotofix’ Nachrichtenbl. Deut. Pflanzenschutzd. 58(2): 46-49.

  • Solomon K.R. Thompson D.G. 2003 Ecological risk assessment for aquatic organisms from over-water uses of glyphosate J. Toxicol. Environ. Health B Crit. Rev. 6(3): 289-324.

  • Steinrucken H.C. Amrhein N. 1980 The herbicide glyphosate is a potent inhibitor of 5-enolpyruvylshikimic acid-3-phosphate synthase Biochem. Biophys. Res. Comm. 94(4): 1207-1212.

  • Tsui M.T.K. Chu L.M. 2004 Comparative toxicity of glyphosate- based herbicides: aqueous and sediment porewater exposures Arch. Environ. Contam. Toxicol. 46(3): 316-323. [USEPA] U.S. Environmental Protection Agency 1993 R.E- .D. Facts: glyphosate US EPA: Office of Pesticide Programs Washington p. 7. [USEPA]

  • U.S. Environmental Protection Agency 1986 Guidance for the reregistration of pesticide products containing glyphosate as the active ingredient US EPA: Office of Pesticide Programs Washington p. 207.

  • Vera-Candioti J. Soloneski S. Larramendy M.L. 2013 Evaluation of the genotoxic and cytotoxic effects of glyphosate- based herbicides in the ten spotted live-bearer fish Cnesterodon decemmaculatus (Jenyns 1842) Ecotoxicol. Environ. Saf. 89: 166-173.

  • Wang Y.S. Jaw C.G. Chen Y.L. 1994 Accumulation of 24- D and glyphosate in fish and water hyacinth Water Air Soil Pollut. 74(3-4): 397-403. [WHO] World Health Organization 1994 Glyphosate. Environmental health criteria 159 WHO: IPCS Geneva p. 177.

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