The problem of losses due to infection with koi herpesvirus (KHV) disease (KHVD) occurred in the 1990s. Since the connection of mass mortality of koi in the United States and Israel with the presence of a new virus of the Alloherpesviridae family, there have been an increasing number of reports of prevalence and pathogenicity of the new disease. The associated research performed led to a detailed description of a new pathogen and its occurrence. Koi herpesvirus quickly spreads in the species Cyprinus carpio, comprising common carp and its ornamental relative – the koi. Currently, cyprinid herpesvirus-3 (CyHV-3) is isolated in all areas where carp and koi carp are bred (12, 14). The Department of Fish Disease and Pathology Laboratory of the National Veterinary Research Institute diagnosed KHV infection in common carp in Poland for the first time in 2004 (1).
A factor having a huge impact on the rate and extent of spread of the infection has been unrestricted trade in koi, often over very large distances (Fig. 1). Besides the possibility of horizontal transmission of the virus directly from fish to fish, the ability for the virus to spread by vectors like water (it being the major abiotic vector) should also be taken into consideration. However, hypothetically, animate vectors, e.g. other fish species, parasitic invertebrates, and piscivorous birds and mammals can also be involved in transmission. The role of different fish species as vectors of virus transmission is well known in the cases of viral haemorrhagic septicaemia virus (VHSV) and infectious haematopoietic necrosis virus (IHNV) (4, 6, 10, 11) but in the case of KHV infection knowledge concerning the scope of vectors is still lacking. In addition, the presence of KHV DNA has been reported (although no infection has been demonstrated) in the following species: Atlantic sturgeon (Acipenser oxyrinchus), blue back ide (Leuciscus idus), common roach (Rutilus rutilus), Eurasian ruffe (Gymnocephalus cernuus), European perch (Perca fluviatilis), hybrid sterlet × beluga (Acipenser ruthenus × Huso huso), rainbow trout (Oncorhynchus mykiss), Russian sturgeon (Acipenser gueldenstaedtii), silver carp (Hypophthalmichthys molitrix), stone loach (Barbatula barbatula), tench (Tinca tinca), swan mussel (Anodonta cygnea), and scud (a crustacean) (Gammarus pulex) (12).
The investigations presented in this article were initiated for better understanding the pathways of CyHV-3 infection transmission in Cyprinus carpio. The study focused upon the role of other fish maintained with common carp in ponds as potential KHV vectors.
Material and Methods
Experimental study. Fish from eight different species (30 fish in each species) were experimentally infected with CyHV-3 (replicated in common carp brain cells – CCB) by immersion in 10 L aquaria. Investigations were carried out on specimens of European bitterling (Rhodeus amarus), brown bullhead (Ameiurus nebulosus), grass carp, Prussian carp (Carassius gibelio), roach, stone moroko (Pseudorasbora parva), tench, and three-spined stickleback (Gasterosteus aculaeatus). The virus suspensions with a titre of 4 × 103 in 2 ml were added to the tank. Control fish (European bitterling, brown bullhead, grass carp, Prussian carp, roach, stone moroko, tench, and three-spined stickleback) were exposed to CCB cell suspension. After a 45 min exposure, both groups were transferred to 350 L tanks with filtered and aerated water at 20°C. Every seven days, two fish per species were euthanised by immersion into a 0.5 g L tricaine solution (Sigma-Aldrich, USA) and samples of the gills, spleen, and kidney were collected. At the same time intervals, samples from control group fish were collected. In the second experimental trial, four times at seven day intervals, 10 infected fish from different species (grass carp, Prussian carp, roach, tench and brown bullhead) were transferred to a separate tank for each time interval with naïve common carp (30 fish per tank). Every day, carp in all four tanks were observed for evidence of any clinical symptoms of KHVD. After three months’ observation, the carp were sacrificed. Sections of their gills, spleen, and kidney were collected into sterile tubes. At the same time, samples from control group fish were also collected.
Study on a traditional common carp farm with KHV history. During outbreaks of KHVD on a farm in eastern Poland, 70% cumulative mortality in common carp was reported. Different fish species from this farm were sampled. All collected fish showed no KHVD symptoms. These samples were collected at intervals of 3, 4, 10, 13, 16, and 22 months after confirmation of the KHV outbreak on the farm. Part of the samples were collected from channels which supply water to and drain it from the farm. Fish from 14 different species (bleak, brown bullhead, European perch, freshwater bream (Abramis brama), goldfish (Carassius auratus), grass carp, ide (Leuciscus idus), northern pike (Esox lucius), Prussian carp, roach, rudd (Scardinius erythrophthalmus), tench, wels catfish (Silurus glanis), and white bream (Blicca bjoerkna)) were used for the study and provided a total of 205 samples. Sections of internal organs from single fish were collected in a single tube.
DNA extraction. The tissue samples were homogenised and total DNA was extracted using a QIAamp DNA Mini Kit (Qiagen, Germany) according to the manufacturer’s instructions. The DNA was eluted in 100 μL of acetate ethylenediaminetetraacetic acid (AE) buffer and stored at −80°C before testing.
Molecular identification. All samples were initially tested by qPCR as described in Commission Implementing Decision (EU) 2015/1554 of 11 September 2015 (5). Virus DNA detection and quantification were performed using real-time qPCR (TaqMan) modified from the original protocol of Gilad et al. (2004) (8). Briefly, qPCR was performed as follows: the forward primer (KHV-86f) was 5′- GACGCC GGAGACCTTGTG -3′; the reverse primer (KHV-163r) was 5′- CGGGTTCTTATTTTTGTCCTTGTT -3′; and the probe (KHV-109p) was 5′-FAM- CTT CCTCTGCTCGGCGAGCACG -3′. Cycling conditions were 1 cycle at 95°C for 15 min and 50 cycles at 94°C for 15 sec and 60°C for 60 sec.
Fish from eight different species were experimentally infected with CyHV-3 by immersion and viral DNA was assayed in samples. It could be detected in two species, tench and roach, in samples taken up to 49 days post infection (dpi). We confirmed KHV DNA up to 42 dpi in grass carp and Prussian carp samples. However, viral DNA was found in samples from three-spined stickleback and stone maroko only up to 14 dpi (Table 1). No clinical symptoms or mortality in fish experimentally infected with KHV were observed to the end of the experiment.
Results of experimental infection of fish from eight different species with CyHV-3
|Three-spined stickleback||Stone moroko||European bitterling||Grass carp||Prussian carp||Tench||Roach||Brown bullhead|
In the second experimental challenge, where fish from different species were transferred at seven dpi to naïve common carp, the first clinical symptoms of KHV occurred after 28 days of cohabitation. Moribund fish presented enophthalmia, over-production of mucus on the skin, and necrosis of the gills (Fig. 1). Carp started dying on the 30th day after transfer of fish from different species and their cumulative mortality reached 90%. In samples from moribund carp the presence of KHV nucleic acids was detected. During three months’ observation of carp from the remaining three groups (where fish from different species were transferred after 14, 21, and 28 dpi), neither clinical symptoms nor mortality were observed. In samples from euthanised fish from the three groups mentioned above, the presence of virus DNA was confirmed (Table 2). In both experimental trials, no clinical symptoms or mortality in fish from different species experimentally infected with KHV were observed and in samples originating from control fish, the presence of KHV was not detected.
Species of fish experimentally infected with KHV and results of virus transfer trial to naïve carp
|Species of fish experimentally infected with KHV via water:||Grass carp, Prussian carp, tench, roach, brown bullhead|
|Time of transfer of different fish species (two fish per species) to naïve common carp (dpi)|
|Mortality in common carp (%)||90||0||0||0|
In samples from different fish species from a traditional common carp farm with a history of KHV, KHV DNA was detected in common carp three months after confirmation of a KHV outbreak. Viral nucleic acids were detected in samples from four fish (two Prussian carp, one tench, and one brown bullhead). All samples collected at the 4, 10, 13, 16, and 22 month time points were negative (Table 3), and in samples collected from channels which supply water to and drain it from the farm no presence of KHV DNA was detected.
Results of examination of different fish species taken from farm with KHV history
|Sampled species:||Bleak, brown bullhead, European perch, freshwater bream, goldfish, grass carp, ide, northern pike, Prussian carp, roach, rudd, tench, wels catfish, white bream|
|Time of sampling after KHV outbreak (months)||Number of positive samples||Number of negative samples|
|3||4 (tench, brown bullhead, Prussian carp)||27|
The results of our experimental and field investigations showed the presence of KHV DNA in species of fish which had contact with infected common carp. According to published data (7), we have confirmed the presence of KHV DNA in 10 different fish species. Bergmann et al. (3) detected KHV by nested PCR in several different varieties of goldfish as well as grass carp, ide, and ornamental catfish (Ancistrus sp.). KHV was detected by PCR in Russian sturgeon and Atlantic sturgeon from fish farms in northern Poland (9). We also confirmed the presence of virus nucleic acids in other fish species reared in polyculture with carp but we also did so in fish that can migrate in and out of a farm. Interestingly, Bergmann et al. (2) reported the replication of KHV in goldfish after experimental infection by immersion, but clearly visible clinical symptoms of KHVD did not occur. In our experimental infection and field findings, we also did not observe any clinical symptoms or mortality caused by CyHV-3 in fish from different species than Cyprinus carpio. We have evidence that other fish species are potential vectors of KHV from literature and our data. In eight different fish species experimentally infected with KHV, we observed that after a short contact with the virus, viral DNA could subsequently be found until 49 dpi in two sampled fishes (tench and roach). However, this period of viral DNA presence was different for each species: for example in samples from three-spined stickleback and stone moroko we had positive results only until 14 dpi. This investigation concerning transfer of the pathogen from vectors to naïve common carp showed that after a long period (four weeks), other fish species can play a role in virus transmission. In the study by Bergmann et al. (2), transfer of infectious virus from goldfish to naïve koi was possible after 60 dpi.
Concluding, in vitro and in vivo studies confirmed that in samples from European bitterling, brown bullhead, grass carp, Prussian carp, roach, stone moroko, tench, and three-spined stickleback which had had short contact time with CyHV-3, viral DNA was detected for a long period of time (from two up to seven weeks). Even after a short contact time of with KHV, other fish can transfer the virus to a naïve population for four weeks. The potential virus transmission periods are different and depend on the fish species.
Antychowicz J. Reichert M. Matras M. Bergmann S.M. Haenen O.: Epidemiology pathogenicity and molecular biology of koi herpesvirus isolated in Poland. Bull Vet Inst Pulawy 2005 49 367–373.
Bergmann S.M. Lutze P. Schutze H. Fischer U. Dauber M. Fichtner D. Kempter J.: Goldfish (Carassius auratus) is a susceptible species for koi herpesvirus (KHV) but not for KHV disease. Bull Eur Assoc Fish Pathol 2010 30 74–84.
Bergmann S.M. Schütze H. Fischer U. Fichtner D. Riechardt M. Meyer K. Schrudde D. Kempter J.: Detection of koi herpes virus (KHV) genome in apparently healthy fish. Bull Eur Ass Fish Pathol 2009 29 145–152.
Bootland L.M. Leong J.C.: Infectious hematopoietic necrosis virus. In: Fish Diseases and Disorders vol 3 Viral Bacterial and Fungal Infections Edited by Woo P.T.K. Bruno D.W. CAB International Oxon UK 1999 pp. 57–121.
Commission Implementing Decision (EU) 2015/1554 of 11 September 2015 laying down rules for the application of Directive 2006/88/EC as regards requirements for surveillance and diagnostic methods. Off J EU L 247/1.
European Food Safety Authority (EFSA). Scientific opinion of the panel on AHAW on a request from the European Commission on aquatic animal species susceptible to diseases listed in the Council Directive 2006/88/EC. EFSA J 2008 808 1–144.
Fabian M. Baumer A. Steinhagen D.: Do wild fish species contribute to the transmission of koi herpesvirus to carp in hatchery ponds? J Fish Dis 2013 36 505–514.
Gilad O. Yun S. Zagmutt-Vergara F.J. Leutenegger C.M. Bercovier H. Hedrick R.P.: Concentrations of a Koi herpesvirus (KHV) in tissues of experimentally infected Cyprinus carpio koi as assessed by real-time TaqMan PCR. Dis. Aquat. Organ. 2004 60 179–187.
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Gilad O., Yun S., Zagmutt-Vergara F.J., Leutenegger C.M., Bercovier H., Hedrick R.P.: Concentrations of a Koi herpesvirus (KHV) in tissues of experimentally infected Cyprinus carpio koi as assessed by real-time TaqMan PCR. Dis. Aquat. Organ. 2004, 60, 179–187.)| false 15521316 10.3354/dao060179
Kempter J. Sadowski J. Schutze H. Fischer U. Dauber M. Fichtner D. Panicz R. Bergmann S.M.: Koi herpesvirus: do Acipenserid restitution programs pose a threat to carp farms in the disease free zones? Acta Ichthyolog Piscator 2009 39 119–126.
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Kempter J., Sadowski J., Schutze H., Fischer U., Dauber M., Fichtner D., Panicz R., Bergmann S.M.: Koi herpesvirus: do Acipenserid restitution programs pose a threat to carp farms in the disease free zones? Acta Ichthyolog Piscator 2009, 39, 119–126.)| false 10.3750/AIP2009.39.2.06
Olesen N.J. Vestergård Jørgensen P.E.: Can and do herons serve as vectors for Egtved virus? Bull Eur Assoc Fish Pathol 1982 2 48.
Peters F. Neukirch M.: Transmission of some fish pathogenic viruses by the heron Ardea cinerea J Fish Dis 1986 9 539–544.
World Organisation for Animal Health (OIE): Manual of diagnostic tests for aquatic animals 7th Edition Chapter 2.3.7 Koi herpesvirus disease. Paris 2016. http://www.oie.int/index.php?id=2439&L=0&htmfile=chapitre_koi_herpesvirus.htm
World Organisation for Animal Health (OIE). World Animal Health Information System (WAHIS). Disease Outbreak Map 2019. http://www.oie.int/wahis_2/public/wahid.php/Diseaseinformation/Diseaseoutbreakmaps