Changes in the activity of ovine blood-derived macrophages stimulated with antimicrobial peptide extract (AMP) or platelet-rich plasma (PRP)

Open access


Introduction: Antimicrobial peptides (AMP) are a large group of innate immune effectors, which apart from antimicrobial activity show immunomodulative properties. Platelet-rich plasma (PRP) is a source of autologous growth factors and is used for stimulation of bone and soft tissue healing. The purpose of this study was to assess the influence of PRP and AMP extract on ovine monocyte-derived macrophage cultures. Material and Methods: The study was conducted on ovine macrophages (Mfs) previously stimulated with LPS or dexamethasone and then with preparations of PRP or AMP. Following activation of the Mfs their morphological and functional features were assessed. Results: The study revealed pro-inflammatory influence of both examined preparations on Mfs cultures on the basis of morphology, ROS generation and arginase activity. Both preparations enhanced the pro-inflammatory response of cultured Mfs. Conclusion: This activity may intensify the antimicrobial action of Mfs, however, in cases of excessive and prolonged inflammation the use of these preparations should be limited.

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • 1. Agier J. Efenberger M. Brzezińska-Błaszczyk E.: Cathelicidin impact on inflammatory cells. Centr Eur J Immunol 2015 40 225–235.

  • 2. Anderson R.C. Rehders M. Yu P.L.: Antimicrobial fragments of the pro-region of cathelicidins and other immune peptides. Biotechnol Lett 2008 30 813–818.

  • 3. Arrepol E.G.S. Pérez C. Escobar A. Ortiz M.C. Tempio Sepúlveda F. Lopez M.N.: Whole platelets induces macrophage programming toward a repair profile characterized by high IL-10 production. Front Immunol Conference Abstract. 11th Congress of the Latin American Association of Immunology - 100 Congreso de la Asociación Colombiana de Alergia Asma e Inmunología. doi: 10.3389/conf.fimmu.2015.05.00015 (2015).

  • 4. Campbell L. Emmerson E. Williams H. Saville C.R. Krust A. Chambon P. Mace K.A. Hardman J.: Estrogen receptor-alpha promotes alternative macrophage activation during cutaneous repair. J Invest Dermatol 2014 134 2447–2457.

  • 5. Campbell L. Saville C.R. Murray P.J. Cruickshank S.M. Hardman J.: Local arginase 1 activity is required for cutaneous wound healing. J Invest Dermatol 2013 133 2461–2470.

  • 6. Cecillio C.A. Costa E.H. Simioni P.U. Gabriel D.L. Tamashiro W.M.S.C.: Aging alters the production of iNOS arginase and cytokines in murine macrophages. Braz J Med Biol Res 2011 44 671–681.

  • 7. Corraliza I.M. Campo M.L. Soler G. Modolell M.: Determination of arginase activity in macrophages: a micromethod. J Immunol Methods 1994 174 231–235.

  • 8. Czakai K. Dittrich M. Kaltdorf M. Müller T. Krappmann S. Schedler A. Bonin M. Dühring S. Schuster S. Speth C. Rambach G. Einsele H. Dandekar T. Löffler K.J.: Influence of platelet-rich plasma on the immune response of human monocyte-derived dendritic cells and macrophages stimulated with Aspergillus fumigatus. International J Med Microbiol 2017 307 95–107.

  • 9. Eligni S. Crisci M. Bono E. Songia P. Tremoli E. Colombo G.I. Coll S.: Human monocyte-derived macrophages spontaneously differentiated in vitro show distinct phenotypes. J Cell Physiol 2013 228 1464–1472.

  • 10. Elmowalid G.: A simple method for generation of functional sheep peripheral blood monocytes-derived-macrophages in vitro. Global Veterinaria 2012 9 590–599.

  • 11. Giles K. Ross K. Rossi A.G. Hotchin N.A. Haslett C. Dransfield I.: Glucocorticoid augmentation of macrophage capacity for phagocytosis of apoptotic cells is associated with reduced p130 Cas expression loss of paxillin/pyk2 phosphorylation and high levels of active Rac. J Immunol 2001 167 976–986.

  • 12. Mantovani A. Sica A. Sozzani S. Allavena P. Vecchi A. Locati M.: The chemokine system in diverse forms of macrophage activation and polarization. Trends Immunol 2004 25 677–686.

  • 13. McPhee J.B. Hancock R.E.W.: Function and therapeutic potential of host defence peptides. J Pep Sci 2005 11 677–687.

  • 14. McWhorter F. Wang T. Nguyen P. Chung T. Liu W.F.: Modulation of macrophage phenotype by cell shape. PNAS 2013 10 17253–17258.

  • 15. Murray P.J. Wynn T.A.: Protective and pathogenic functions of macrophage subsets. Nat Rev Immunol 2011 11 723–737.

  • 16. Pelillo C. Benincasa M. Scocchi M. Gennaro R. Tossi A. Pacor S.: Cellular internalization and cytotoxicity of the antimicrobial proline-rich peptide Bac7(1-35) in monocytes/ macrophages and its activity against phagocytosed Salmonella typhimurium. Protein Peptide Lett 2014 21 382–390.

  • 17. Ploeger D.T.A. Hosper N.A. Schipper M. Koerts J.A. de Rond S. Bank R.A.: Cell plasticity in wound healing: paracrine factors of M1/M2 polarized macrophages influence the phenotypical state of dermal fibroblasts. Cell Commun Signal 2013 11 29.

  • 18. Porcheray F. Viaud S. Rimaniol A.C. Leone C. Samah B. Dereuddre-Bosquet N. Dormont D. Gras G.: Macrophage activation switching: an asset for the resolution of inflammation. Clin Experiment Immunol 2005 142 481–489.

  • 19. Sang Y. Blecha F.: Porcine host defense peptides: expanding repertoire and functions. Develop Comp Immunol 2009 33 334–343.

  • 20. Schnabel L.V. Mohammed H.O. Miller B.J. McDermott W.G. Jacobson M.S. Santangelo K.S. Fortier L.A.: Platelet rich plasma (PRP) enhances anabolic gene expression patterns in flexor digitorum superficialis tendons. J Orthop Res 2007 25 230–240.

  • 21. Scull C.M. Hays W.D. Fischer H.: Macrophage pro-inflammatory cytokine secretion is enhanced following interaction with autologous platelets. J Inflamm 2010 7 53.

  • 22. Spiller K.L. Anfang R.R. Spiller K.J. Ng J. Nakazawa K.R. Daulton J.W. Vunjak-Novakovic G.: The role of macrophage phenotype in vascularization of tissue engineering scaffold. Biomaterials 2014 35 4477–4488.

  • 23. Szponder T. Wessely-Szponder J. Świeca M. Smolira A. Gruszecki T.: The combined use of ozone therapy and autologous platelet-rich plasma as an alternative approach in foot rot treatment in sheep. A preliminary study. Small Rumin Res 2017 156 50–56.

  • 24. Szponder T. Wessely-Szponder J. Smolira A. Evaluation of platelet-rich plasma and neutrophil antimicrobial extract as two autologous blood-derived agents. Tissue Eng Regen Med 2017 14 287–296.

  • 25. Wessely-Szponder J. Szponder T. Bobowiec R.: Different activation of monocyte-derived macrophages by antimicrobial peptides at a titanium tibial implantation in rabbits. Res Vet Sci 2017 115 201–210.

  • 26. Zizzo G. Cohen P.L.: IL-17 stimulates differentiation of human anti-inflammatory macrophages and phagocytosis of apoptotic neutrophils in response to IL-10 and glucocorticoids. J Immunol 2013 190 5237–5246 doi:10.4049/jimmunol.1203017.

Journal information
Impact Factor

IMPACT FACTOR J Vet Res 2018: 0.829
5-year IMPACT FACTOR: 0.938

CiteScore 2018: 0.68

SCImago Journal Rank (SJR) 2018: 0.291
Source Normalized Impact per Paper (SNIP) 2018: 0.501

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 143 143 18
PDF Downloads 97 97 9