Introduction: Laryngeal swab samples collected from three waterfowl slaughterhouses in central Taiwan were cultured and suspected isolates of Riemerella anatipestifer were identified by API 20NE and 16S rDNA PCR.
Material and Methods: Serum agglutination was used for serotyping, and antimicrobial susceptibility was tested.
Results: Seventy-six R. anatipestifer isolates were detected, and the prevalences in the ducks and geese were 12.3% (46/375) and 8.0% (30/375), respectively. The positive isolation rates were 65.6% for all arriving waterfowl, 76.0% for birds in the holding area, 1.6% for defeathered carcasses, but zero for degummed carcasses. A PCR examination detected R. anatipestifer in the slaughtering area frequently. Serotype B was dominant in both duck (34.8%) and goose (46.7%) isolates, but the wide serotype distribution may very well impede vaccination development. All isolates were resistant to colistin, and 79.7% were resistant to more than three common antibiotics.
Conclusion: The results proved that most ducks had encountered antibiotic-resistant R. anatipestifer in rearing, which suggests that the bacterium circulates in asymptomatic waterfowl. It is worth noting that most waterfowl farms were found to harbour R. anatipestifer, and contaminated slaughterhouses are a major risk factor in its spread. Effective prevention and containment measures should be established there to interrupt the transmission chain of R. anatipestifer.
2. Cha S.Y. Seo H.S. Wei B. Kang M. Roh J.H. Yoon R.H. Kim J.H. Jang H.K.: Surveillance and characterization of Riemerella anatipestifer from wild birds in South Korea. J Wildl Dis 2015 51 341–347.
3. Chang C.F.: Pathogenesis of Pasteurella anatipestifer infection in ducks and drug sensitivity. Taiwan J Vet Med Anim Hus 1984 43 40–46.
4. Chen C.L. Wang S.T. Chu C. Wang S.H.: Comparison of four molecular typing methods for Riemerella anatipestifer. Taiwan Vet J 2015 41 177–185.
5. Chen Y.P. Lee S.H. Chou C.H. Tsai H.J.: Detection of florfenicol resistance genes in Riemerella anatipestifer isolated from ducks and geese. Vet Microbiol 2012 154 325–331.
6. Chen Y.P. Lee S.H. Tsai H.J.: Serotyping of Riemerellar anatipestifer isolates from waterfowl in Taiwan between 2008 and 2012. Exp Rep Taiwan AHRI 2013 48 21–28.
7. Chu C.Y. Liu C.H. Liou J.J. Lee J.W. Cheng L.T.: Development of a subunit vaccine containing recombinant Riemerella anatipestifer outer membrane protein A and CpG ODN adjuvant. Vaccine 2015 33 92–99.
8. Cochran W.G.: Sampling Techniques Wiley New York. 1977.
9. Cooper G.L.: Pasteurella anatipestifer infections in California turkey flocks: circumstantial evidence of a mosquito vector. Avian Dis 1989 33 809–815.
10. Davis M.A. Winn J.F.: Poultry disease manual. Texas Cooperative Extension Texas A & M University System College Station Texas. 2006.
11. Heddleston K.L.: Infectious serositis. In: Diseases of poultry. Edited by Hofstad M.S. Calnek B.W. Biester H.E. American Association of Avian Pathologists Iowa State University Press Ames 1972 pp. 246–251.
12. Hess C. Enichlmayr H. Jandreski-Cvetkovic D. Liebhart D. Bilic I. Hess M.: Riemerella anatipestifer outbreaks in commercial goose flocks and identification of isolates by MALDI-TOF mass spectrometry. Avian Pathol 2013 42 151–156.
13. Hu Q. Han X. Zhou X. Ding S. Ding C. Yu S.: Characterization of biofilm formation by Riemerella anatipestifer. Vet Microbiol 2010 144 429–436.
14. Huang C.C.: The natural transmission serotypes antimicrobial susceptibility of Riemerella anatipestifer and combinational infection with circoviruses in waterfowl. Department of Veterinary Medicine. National Chiayi University Chiayi Taiwan. 2008.
15. Layton H.W. Sandhu T.S.: Protection of ducklings with a broth-grown Pasteurella anatipestifer bacterin. Avian Dis 1984 28 718–726.
16. Leavitt S. Ayroud M.: Riemerella anatipestifer infection of domestic ducklings. Can Vet J 1997 38 113.
17. Leibovitz L.: A survey of the so-called “anatipestifer syndrome”. Avian Dis 1972 16 836–851.
18. Li Y.L.: Sequence analysis of plasmids and application to detection of antibiotics resistance genes in Riemerella anatipestifer isolated in Taiwan. Department of Veterinary Medicine. National Chung Hsing University Taichung. 2004. p. 75.
19. Liu C.K. Chen C.M. Chang T.C. Chang W.F. Chang S.K. Kuo C.C. Lin J.S. Chu C.Y.: Handbook of veterinary drugs commonly used for poultry. Bureau of Animal and Plant Health Inspection and Quarantine COA EY Taipei Taiwan R.O.C. 2009.
20. Liu H. Wang X. Ding C. Han X. Cheng A. Wang S. Yu S.: Development and evaluation of a trivalent Riemerella anatipestifer-inactivated vaccine. Clin Vaccine Immunol 2013 20 691–697.
21. Liu Y.Y. Wang Y. Walsh T.R. Yi L.X. Zhang R. Spencer J. Doi Y. Tian G. Dong B. Huang X. Yu L.F. Gu D. Ren H. Chen X. Lv L. He D. Zhou H. Liang Z. Liu J.H. Shen J.: Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China: a microbiological and molecular biological study. Lancet Infect Dis 2016 16 161–168.
22. Loh H. Teo T.P. Tan H.C.: Serotypes of ‘Pasteurella’ anatipestifer isolates from ducks in Singapore: a proposal of new serotypes. Avian Pathol 1992 21 453–459.
23. Magwedere K. Rauff D. De Klerk G. Keddy K.H. Dziva F.: Incidence of nontyphoidal Salmonella in food-producing animals animal feed and the associated environment in South Africa 2012-2014. Clin Infect Dis 2015 61 283–289.
24. Pathanasophon P. Sawada T. Pramoolsinsap T. Tanticharoenyos T.: Immunogenicity of Riemerella anatipestifer broth culture bacterin and cell-free culture filtrate in ducks. Avian Pathol 1996 25 705–719.
25. Pathanasophon P. Sawada T. Tanticharoenyos T.: New serotypes of Riemerella anatipestifer isolated from ducks in Thailand. Avian Pathol 1995 24 195–199.
26. Phonvisay M. Liou J.J. Cheng L.T. Chen Y.P. Wu H.C. Liu C.H. Lee J.W. Chu C.Y.: Survey of a Riemerella anatipestifer outbreak in southern taiwan duck farms. Taiwan Vet J 2017 43 165–170.
27. Ryll M. Christensen H. Bisgaard M. Christensen J.P. Hinz K.H. Köhler B.: Studies on the prevalence of Riemerella anatipestifer in the upper respiratory tract of clinically healthy ducklings and characterization of untypable strains. J Vet Med B Infect Dis Vet Public Health 2001 48 537–546.
28. Sandhu T.S.: Immunogenicity and safety of a live Pasteurella anatipestifer vaccine in White Pekin ducklings: laboratory and field trials. Avian Pathol 1991 20 423–432.
29. Sandhu T.S.: Riemerella anatipestifer infection. In: Diseases of Poultry edited by Saif Y.M. Barnes H.J. Glisson J.R. Fadly A.M. McDougald L.R. Swayne D.E. Iowa State Press Ames Iowa. 2003. pp. 676–682.
30. Tian K.G. Gui Y.P.: Research on infectious sinusitis in white Beijing ducklings. I. Epidemiology and pathology. Xu Mu Shou Yi Xue Bao 1990 21 327–331.
31. Tsai H.J. Liu Y.T. Tseng C.S. Pan M.J.: Genetic variation of the ompA and 16S rRNA genes of Riemerella anatipestifer. Avian Pathol 2005 34 55–64.
32. Umaraw P. Prajapati A. Verma A.K. Pathak V. Singh V.P.: Control of campylobacter in poultry industry from farm to poultry processing unit: A review. Crit Rev Food Sci Nutr 2017 57 659–665.
33. Wayne P.: CLSI. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Fifth Informational Supplement. CLSI Document M100-S25 Clinical and Laboratory Standards Institute. 2015.
34. Yu C.Y. Liu Y.W. Chou S.J. Chao M.R. Weng B.C. Tsay J.G. Chiu C.H. Ching Wu C. Long Lin T. Chang C.C. Chu C.: Genomic diversity and molecular differentiation of Riemerella anatipestifer associated with eight outbreaks in five farms. Avian Pathol 2008 37 273–279.