Introduction: The influence of feeding genetically modified MON 810 hybrid maize on the growth and haematological and biochemical indices of rats was tested.
Material and Methods: Two conventional (non-GM) and two test (MON 810) lines of maize were used in semi-purified diets at the level of 40% w/w. The non-GM I, MON 810 I, non-GM II, and MON 810 II maize lines were near-isogenic. A total of 40 male 6-week-old Wistar-derived rats were assigned to four equal feeding groups corresponding to the four maize lines for 16 weeks. Overall, health, body weight gain, clinical pathology parameters, gross changes, and appearance of tissues were compared between groups.
Results: There were no statistically significant differences in the weight gain or relative organ weights of rats, but there were some non diet-related histopathological changes in the liver, kidneys, and spleen. Except for creatinine level, no diet-related effects were observed in haematology or most of the biochemical indices. Transgenic DNA of MON 810 maize was not detected in the tissues or faeces nor in the DNA of E. coli isolated from the rectum digesta of rats given transgenic feeds. In our experiment, various metabolic indices of rats fed non-GM diets or genetically modified (MON 810) maize for 16 weeks were similar. No adverse nutrition-related health effects were detected.
Conclusion: MON 810 maize seems to be as safe as the conventional maize lines.
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1. Appenzeller L.M., Munley S.M., Hoban D., Sykes G.P., Malley L.A., Delaney B.: Subchronic feeding study of grain from herbicide-tolerant maize DP-O9814O-6 in Sprague-Dawley rats. Food Chem Toxicol 2009, 47, 2269–2280.
2. Baranowski A., Rosochacki S., Parada R., Jaszczak K., Zimny J., Połoszynowicz J.: The effect of diet containing genetically modified triticale on growth and transgenic DNA fate in selected tissues of mice. Animal Sci Papers Rep 2006, 24, 129–142.
3. Bednarek D., Dudek K., Kwiatek K., Świątkiewicz M., Świątkiewicz S., Strzetelski J.: Effect of a diet composed of genetically modified feed components on the selected immune parameters in pigs, cattle, and poultry. Bull Vet Inst Pulawy 2013, 57, 209–2017. doi:10.2478/bvip-2013-0038.
4. Chen X., Zhuo Q., Piao J., Yang X.: Immunotoxicologic assessment of transgenic rice. J Hyg Res 2004, 33, 770–780.
5. Czerwiński J., Bogacki M., Jalali B.M., Konieczka P., Smulikowska S.: The use of genetically modified Roundup Ready soyabean meal and genetically modified MON 810 maize in broiler chicken diets. Part 1. Effects on performance and blood lymphocyte subpopulations. J Anim Feed Sci 2015, 24, 134–143.
6. El-Shamei Z.S., Gab-Alla A.A., Shatta A.A., Moussa E.A. Rayan A.M.: Histopathological changes in some organs of male rats fed on genetically modified corn (Ajeeb YG). J Am Sci 2012, 8, 684–696.
7. Flachowsky G., Chesson A., Aulrich K.: Animal nutrition with feeds from genetically modified plants. Arch Anim Nutr 2005, 59, 1, 1–40.
8. Halle I., Aulrich K., Flachowsky G.: Four generations of feeding of GMO-corn to breeder quail. (Fütterung von gentechnisch verändertem Mais an Zuchtwachtein über vier Generationen). Proc Soc Nutr Physiol 2004, 13, 124.
9. Hammond B.G., Dudek R., Lemen J., Nemeth M.A., Results of a 13 week safety assurance study with rats fed grain from glyphosate tolerant maize. Food Chem Toxicol 2004, 42, 1003–1014.
10. Hammond B.G., Lemon J., Dudek R., Ward D., Jiang C., Nemeth M.A., Burns J.: Results of a 90-day safety assurance study with rats fed grain from maize rootworm – protected maize. Food Chem Toxicol 2006, 44, 147–160.
11. Healy C., Hammond B., Kirkpatrick J.: Results of a 13-week safety assurance study with rats fed grain from corn rootwormprotected, glyphosate-tolerant MON 88017 corn. Food Chem Toxicol 2008, 46, 2517–2524.
12. Jennings J.C., Albee L.D., Kolwyck D.C., Surber J.B., Taylor M.L., Hartnell G.F., Lirette R.P., Glenn K.C. Attempts to detect transgenic and endogenous plant DNA and transgenic protein in muscle from broilers fed YieldGard corn borer corn. Poultry Sci 2003, 82, 371–380.
13. Kharazmi M., Bauer T., Hammes W., Hertel C.: Effect of food processing on the fate of DNA with regard to degradation and transformation capability in Bacillus subtilis. Syst Appl Microbiol 2003, 26, 495–501.
14. Kılıç A., Akay T.: A three generation study with genetically modified Bt maize in rats, biochemical and histopathological investigation. Food Chem Toxicol 2007, 46, 1164–1170.
15. Kılıçgün H., Gursul C., Sunar M., Gökşen, G.: The comparative effects of genetically modified maize and conventional maize on rats. J Clin Anal Med 2013, 4, 136–139.
16. Kosieradzka I., Sawosz E., Szopa J., Bielecki W.: Potato genetically modified by 14-3-3 protein repression in growing rat diets. Part II. Health status of experimental animals. Pol J Food Nutr Sci 2008, 58, 377–382.
17. Kosieradzka I., Vasko V., Szwacka M., Przybysz A., Fiedorowicz Sz.: Evaluation of the possibility of horizontal gene transfer accumulation of transgenic DNA from the diet in the bodies of rats. J Anim Feed Sci 2010, 19, 307–315.
18. Oraby H., Kandil M., Shaffie N., Ghaly I.: Biological impact of feeding rats with a genetically modified-based diet. Turk J Biol 2015, 39, 265–275.
19. Poulsen M., Kroghsbo S., Schrøder M., Wilcks A., Jacobsen H., Miller A., Frenzel T., Danier J., Rychlik M., Shu Q.: A 90-day safety study in Wistar rats fed genetically modified rice expressing snowdrop lectin Galanthus nivalis (GNA). Food Chem Toxicol 2007, 3, 350–363.
20. Raju M.V.L.N., Rama Rao S.V., Radhika K., Panda A.K.: Effect of amount and source of supplemental dietary vegetable oil on broiler chickens exposed to aflatoxicosis. Brit Poultry Sci 2005, 46, 587–594.
21. Sartowska K.E., Korwin-Kossakowska A., Seder G.: Genetically modified crops in a 10-generation feeding trial on Japanese quails. Evaluation of its influence on birds’ performance and body composition. Poult Sci 2015, 12, 2909–2916.
22. Schroder M., Poulsen M., Wilcks A., Kroghsbo S., Miller A., Frenzel T., Danier J., Rychlik M., Emami K., Gatehouse A., Shu Q., Engel K.H., Altosaar I., Knudsen I.: A 90-day safety study of genetically modified rice expressing Cry 1 Ab protein (Bacillus thuringensis toxin) in Wistar rats. Food Chem Toxicol 2007, 45, 339–349.
23. Świątkiewicz M., Bednarek D., Markowski J., Hanczakowska E., Kwiatek K.: Effect of feeding genetically modified maize and soybean meal to sows on their reproductive traits, haematological indices and offspring performance. Bull Vet Inst Pulawy 2013, 57, 413–418.
24. Świątkiewicz M., Hanczakowska E., Twardowska M., Mazur M., Kwiatek K., Kozaczyński W., Świątkiewicz S., Sieradzki M.: Effect of genetically modified feeds on fattening results and transfer of transgenic DNA to swine tissues. Bull Vet Inst Pulawy 2011, 55, 121–125.
25. Świątkiewicz S., Koreleski J., Arczewska-Włosek A., Świątkiewicz M., Twardowska M., Markowski J., Mazur M., Sieradzki Z., Kwiatek K.: Detection of transgenic DNA from Bt maize and herbicide tolerant soybean meal in tissues, eggs, and digestive tract content of laying hens fed diets containing genetically modified plants. Ann Anim Sci 2011, 11, 1, 413–424.
26. Świątkiewicz S., Twardowska M., Markowski J., Mazur M., Sieradzki Z., Kwiatek K.: Fate of transgenic DNA From Bt Maize and Roundup Ready Soybean meal in broilers fed GMO feed. Bull Vet Inst Pulawy 2010, 54, 237–242.
27. Taylor M.L., Stanisiewski E.P., Riordan S.G., Nemeth M.A., George B., Hartnell G.F.: Comparison of broiler performance when fed diets containing Roundup Ready (event RT73, nontransgenic control, or commercial canola meal. Poultry Sci 2004, 83, 456–461.
28. Tudisco R., Lombardi P., Bovera F., d Angelo D., Cutrignelli M.I., Mastellone V., Terzi V., Avallone L., Infascelli F.: Genetically modified soya bean in rabbit feeding, detection of DNA fragments and evaluation of metabolic effects by enzymatic analysis. Anim Sci 2006, 82, 193–199.
29. de Vendômois S.F., Roullier F., Cellier D., Séralini G.E.: A comparison of the effects of three GM corn varieties on mammalian health. Int J Biol Sci 2009, 5, 706–726.
30. Weiss J., Ros-Chumillas M., Pena L., Egea-Cortines M.: Effect of storage and processing on plasmid, yeast and plant genomic DNA stability in juice from genetically modified oranges. J Biotechnol 2007, 128, 194–199.