Pathological alterations in respiratory system during co-infection with low pathogenic avian influenza virus (H9N2) and Escherichia coli in broiler chickens

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Abstract

Introduction: Despite the advancements in the field, there is a lack of data when it comes to co-infections in poultry. Therefore, this study was designed to address this issue. Material and Methods: Broiler birds were experimentally infected with E. coli (O78) and low pathogenic avian influenza (LPAI) strain, alone or in combination. The experimental groups were negative control. Results: The infected birds showed most severe clinical signs in E. coli+LPAI group along with a significant decrease in weight and enhanced macroscopic and microscopic pathological lesions. The survival rate was 60%, 84%, and 100% in birds inoculated with E. coli+LPAI, E. coli, and LPAI virus alone, respectively. The results showed that experimental co-infection with E. coli and H9N2 strain of LPAI virus increased the severity of clinical signs, mortality rate, and gross lesions. The HI titre against LPAI virus infection in the co-infected group was significantly higher than the HI titre of LPAI group, which may indicate that E. coli may promote propagation of H9N2 LPAI virus by alteration of immune response. Conclusion: The present study revealed that co-infection with E. coli and H9N2 LPAI virus caused more serious synergistic pathogenic effects and indicates the role of both pathogens as complicating factors in poultry infections.

References

  • 1. Azizpour A., Goodarzi H., Charkhkar S., Momayez R., Hablolvarid M.H.: Study on clinical aspects of SPF chickens infected with Ornithobacterium rhinotracheale followed by H9N2 avian influenza. Eur J Exp Biol 2013, 3, 186-189.

  • 2. Bano S., Naeem K., Malik S.A.: Evaluation of pathogenic potential of avian influenza virus serotype H9N2 in chickens. Avian Dis 2003, 47, 817-822.

  • 3. Costa-Hurtado M., Afonso C.L., Miller P.J., Spackman E., Kapczynski D.R., Swayne D.E., Shepherd E., Smith D., Zsak A., Pantin-Jackwood M.: Virus interference between H7N2 low pathogenic avian influenza virus and lentogenic Newcastle disease virus in experimental co-infections in chickens and turkeys. Vet Res 2014, 45, 1-11.

  • 4. Fatima Z., Khan MA., Ahmad MUD., Muhammad K., Khwaja KN., Khan A., Anwar Z., Ahad A., Mahmood A.:. Cross sectional survey of live bird markets, and zoo birds for circulating influenza subtypes in Pakistan. Pak Vet J, 2017, 37, 185-189.

  • 5. Jirjis F.F., Noll S.L., Halvorson D.A., Nagaraja K.V., Martin F., Shaw, D.P.: Effects of bacterial coinfection on the pathogenesis of avian pneumovirus infection in turkeys. Avian Dis 2004, 48, 34-49.

  • 6. Mahmood MS., Amir HW., Abbas RZ., Rafique A., Aslam B.: Evaluation of antiviral activity of Azadirachta indica (Neem) bark extract against Newcastle disease virus. Pak Vet J 2017, 37: 1-4.

  • 7. Nili H., Asasi K.: Natural cases and an experimental study of H9N2 avian influenza in commercial broiler chickens of Iran. Avian Pathol 2002, 31, 247-252.

  • 8. Nili H., Asasi K.: Avian influenza (H9N2) outbreak in Iran. Avian Dis 2003, 47, 828-831.

  • 9. Pan Q., Liu A., Zhang F., Ling Y., Ou C., Hou N., He C.: Co-infection of broilers with Ornithobacterium rhinotracheale and H9N2 avian influenza virus. BMC Vet Res 2012, 8, 104-110.

  • 10. Pu J., Yu L.F., Zhe W., Bo M., Earl G.B., Jin H.L.: Pathogenicity of H3N8 influenza viruses isolated from domestic ducks in chickens with or without Escherichia coli coinfection. Avian Dis 2012, 597-600.

  • 11. Rautenschlein S., Miller R.L., Sharma J.M.: 1998. Interferon induction in turkeys by oral administration of the imidazoquinolinamine S-28828 and modulation of the pathogenesis of Escherichia coli. Vet Immunol Immunopathol 1998, 66, 127-141.

  • 12. Rashid F., Abbas MA., Siddique N., Rafique S., Ahmed S., Mehmood F., Shah A., Suleman, M., Roomi, S., Naeem K.: Induction of immunosuppression in broiler chicken upon co-infection of avian adenovirus-4 with low pathogenic avian influenza H9N2. Pak Vet J, 2017, 37(3), 311-315.

  • 13. Stipkovits L., Laszlo E., Vilmos P., Andrea B., Ervin P., Maria S., Susan S., Bela D.: Effect of low-pathogenicity influenza virus H3N8 infection on Mycoplasma gallisepticum infection of chickens. Avian Pathol 2012, 41, 51-57

  • 14. Suarez D.L.: Influenza A virus. In: Avian Influenza. edited by Swayne D.E., Blackwell Publishing, Ames 2008, pp. 3−22.

  • 15. Swayne D.E., Halvorson D.A.: Influenza. In: Diseases of Poultry. Edited by Saif Y.M., Glisson J.R., McDougald L.R., Nolan L.K., Swayne D.E., Blackwell Publishing, Ames 2008, pp. 153-184.

  • 16. Tashiro M., Ciborowski P., Klenk H.D., Pulverer G., Rott R.: Role of staphylococcus protease in the development of influenza pneumonia. Nature 1987, 325, 536-537.

  • 17. Umar S., Sarfraz S., Mushtaq A., Attique M.: Emerging threat of H9N2 viruses in poultry of Pakistan and vaccination strategy. World Poult Sci J 2016, 3, 1-10.

  • 18. Umar S., Younus M., Rehman M.U., Aslam A., Shah M.A.A., Munir M.T., Hussain S., Iqbal F., Fiaz M., Ullah S.: Role of aflatoxin toxicity on transmissibility and pathogenicity of H9N2 avian influenza virus in turkeys. Avian Pathol 2015, 44, 305-310.

  • 19. Wibowo M.H., Anggoro D., Amanu S., Wahyuni A., Untari T., Artanto S., Asmara W.: Receptor binding and antigenic site analysis of hemagglutinin gene fragments of avian influenza virus serotype H5N1 isolated from Indonesia. Pak Vet J 2017, 37, 123-128.

  • 20. Xu X., Chen X., Gao S., Zhao L.: Pathogenicity of FtsK mutant of avian pathogenic Escherichia coli. J Vet Res 2016, 60, 13-18.

Journal of Veterinary Research

formerly Bulletin of the Veterinary Institute in Pulawy

Journal Information


IMPACT FACTOR Bull Vet Inst Pulawy 2016: 0.462

CiteScore 2016: 0.46

SCImago Journal Rank (SJR) 2015: 0.230
Source Normalized Impact per Paper (SNIP) 2015: 0.383

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