Abstract
Introduction: A novel to Europe Schmallenberg virus (SBV) causes clinical disease manifested by reproduction disorders in farm ruminants. In free-living ruminants, SBV antibodies as well as the virus were detected. Recent studies also revealed SBV antibodies in wild boars. The study investigates SBV antibodies occurring in wild boars in Poland at the peak of recent virus epidemics in the country.
Material and Methods: Samples collected from 203 wild boars culled during the 2012/2013 and 2013/2014 hunting season were serologically tested using multi-species cELISA. Attempted neutralisation tests failed due to poor serum quality. RT-PCR was implemented in seropositive and doubtful animals.
Results: Two samples collected from wild boar in the winter of 2013 gave a positive result in ELISA, while another two from the 2012/2013 hunting season were doubtful. No SBV RNA was detected in spleen and liver tissues.
Conclusion: Low SBV seroprevalence in wild boars, despite high incidence of SBV infections occurring simultaneously in wild ruminants, suggests that boars are unlikely to be a significant reservoir of the virus in the sylvatic environment in Poland.
References
1. Alcaide M., Rico C., Ruiz S., Soriguer R., Muñoz J., Figuerola J.: Disentangling vector-borne transmission networks: a universal DNA barcoding method to identify vertebrate hosts from arthropod bloodmeals. PLoS One 2009, 4, e7092.
2. Boadella M., Gortázar C.: Effect of haemolysis and repeated freeze-thawing cycles on wild boar serum antibody testing by ELISA. BMC Res Notes 2011, 4, 498–509.
3. Bartsch S., Bauer B., Wiemann A., Clausen P.H., Steuber S.: Feeding patterns of biting midges of the Culicoides obsoletus and Culicoides pulicaris groups on selected farms in Brandenburg, Germany. Parasitol Res 2009, 105, 373–380.
4. Central Statistical Office of Poland – Forestry Statistical Yearbook 2013. http://stat.gov.pl/obszary-tematyczne/rolnictwo-lesnictwo/lesnictwo/lesnictwo-2016,1,12.html.
5. Chang C.C.: Bunyaviruses. In: Diseases of Swine, edited by J.J. Zimmerman, Wiley-Blackwell, 2012, pp. 490–492.
6. Desmecht D., Garigliany M.M., Beer M., Schirrmeier H., Paternostre J., Volpe R., Linden A.: Detection of antibodies against Schmallenberg virus in wild boars, Belgium, 2010–2012. UIGB congress, 2013, Bruxelles, Belgium.
7. Deutscher Jagdverband, Jagdstatistik. https://www.jagdverband.de/node/3304.
8. European Food Safety Authority: “Schmallenberg” virus: analysis of the epidemiological data. (May 2013) Supporting Publications 2013, p. 22. www.efsa.europa.eu/publications.
9. Fenati M., Armaroli E., Corrain R., Guberti V.: Indirect estimation of porcine parvovirus maternal immunity decay in free-living wild boar (Sus scrofa) piglets by capture-recapture data. Vet J 2009, 180, 262–264.
10. Grochowska M., Larska M., Lechowski L., Żmudziński J.F.: Abundance and activity periods of Culicoides obsoletus (Ceratopogonidae, Insecta), a vector for arboviruses occurring in Poland, in the years 2008–2014. Annual multidisciplinary conference in Florence, IJAS Conference Series, 2015, Florence, Italy.
11. Gutiérrez-Guzmán A.V., Vicente J., Sobrino R., Perez-Ramírez E., Llorente F., Höfle U.: Antibodies to West Nile virus and related flaviviruses in wild boar, red foxes and other mesomammals from Spain. Vet Microbiol 2012, 159, 291–729.
12. Hamblin C., Anderson E.C., Jago M., Mlengeya T., Hipji K.: Antibodies to some pathogenic agents in free-living wild species in Tanzania. Epidemiol Infect 1990, 105, 585–594.
13. Huang C.C., Huang T.S., Deng M.C., Jong M.H., Lin S.Y.: Natural infections of pigs with akabane virus. Vet Microbiol 2003, 94, 1–11.
14. Johnson N., Voller K., Phipps L.P., Mansfield K., Fooks A.R.: Rapid molecular detection methods for arboviruses of livestock of importance to northern Europe. J Biomed Biotechnol 2012, doi: 10.1155/2012/719402.
15. Kęsik-Maliszewska J., Krzysiak M.K., Grochowska M., Lechowski L., Larska M.: Entomological monitoring as a part of epizootic surveillance of Schmallenberg virus circulation in the Bialowieza National Park breeding reserves. International conference Wisent in the forests of Pszczyna- 150 year of breeding, 2015, Pszczyna, Poland.
16. Kirkeby C., Bødker R., Stockmarr A., Lind P., Heegaard P.M.: Quantifying dispersal of European Culicoides (Diptera: Ceratopogonidae) vectors between farms using a novel mark-release-recapture technique. PLoS ONE 2013, 8, e61269.
17. Krzysiak M.K., Iwaniak W., Kęsik-Maliszewska J., Olech W., Larska M.: Serological study of exposure to selected arthropod-borne pathogens in European bison (Bison bonasus) in Poland. Transbound Emerg Dis 2016, doi: 10.1111/tbed.12524.
18. Laloy E., Braud C., Bréard E., Kaandorp J., Bourgeois A., Kohl M., Meyer G., Sailleau C., Viarouge C., Zientara S., Chai N.: Schmallenberg virus in zoo ruminants, France and the Netherlands. Emerg Infect Dis 2016, 22, 2201–2203.
19. Larska M., Kęsik-Maliszewska J., Kuta A.: Spread of Schmallenberg virus infections in the ruminants in Poland between 2012 and 2013. Bull Vet Inst Pulawy 2014, 58, 169–176.
20. Larska M., Kęsik-Maliszewska J., Żmudziński J.F.: Current Schmallenberg virus epidemiological situation in Poland - serological and entomological surveillance. 10th Annual Meeting EPIZONE, 2016, Madrid, Spain.
21. Larska M., Krzysiak M.K., Kęsik-Maliszewska J., Rola J.: Cross-sectional study of Schmallenberg virus seroprevalence in wild ruminants in Poland at the end of the vector season of 2013. BMC Vet Res 2014, 10, 967–973.
22. Lim S.I., Kweon C.H., Tark D.S., Kim S.H., Yang D.K.: Sero-survey on Aino, Akabane, Chuzan, bovine ephemeral fever and Japanese encephalitis virus of cattle and swine in Korea. J Vet Sci 2007, 8, 45–49.
23. Molenaar F.M., La Rocca S.A., Khatri M., Lopez J., Steinbach F., Dastjerdi A.: Exposure of Asian elephants and other exotic ungulates to Schmallenberg virus. PLoS ONE 2015, 10, e0135532.
24. Mouchantat S., Wernike K., Lutz W., Hoffmann B., Ulrich R.G., Börner K., Wittstatt U., Beer M.: A broad spectrum screening of Schmallenberg virus antibodies in wildlife animals in Germany. Vet Res 2015, doi: 10.1186/s13567-015-0232-x.
25. Müller T., Teuffert J., Staubach C., Selhorst T., Depner K.R.: Long-term studies on maternal immunity for Aujeszky’s disease and classical swine fever in wild boar piglets. J Vet Med B Infect Dis Vet Public Health 2005, 52, 432–436.
26. Poskin A., Van Campe W., Mostin L., Cay B., De Regge N.: Experimental Schmallenberg virus infection of pigs. Vet Microbiol 2014, 170, 398–402.
27. Rossi S., Viarouge C., Faure E., Gilot-Fromont E., Gache K., Gibert P., Verheyden H., Hars J., Klein F., Maillard D., Gauthier D., Game Y., Pozet F., Sailleau C., Garnier A., Zientara S., Bréard E.: Exposure of wildlife to the Schmallenberg virus in France (2011-2014): higher, faster, stronger (than bluetongue)! Transbound Emerg Dis 2015, doi: 10.1111/tbed.12371.
28. Sailleau C., Boogaerts C., Meyrueix A., Laloy E., Bréard E., Viarouge C., Desprat A., Vitour D., Doceul V., Boucher C., Zientara S., Nicolier A., Grandjean D.: Schmallenberg virus infection in dogs, France, 2012. Emerg Infect Dis 2013, 19, 1896–1898.
29. Swanson D.A., Turnbull M.W.: Molecular identification of bloodmeals from Culicoides Latreille (diptera: Ceratopogonidae) in the Southeastern U.S.A. Proc Entomol Soc Washington 2014, 116, 351–357.
30. Stokes J.E., Baylis M., Duncan J.S.: A freedom from disease study: Schmallenberg virus in the south of England in 2015. Vet Rec 2016, 179, 435–439.
31. Wensman J.J., Blomqvist G., Hjort M., Holst B.S.: Presence of antibodies to Schmallenberg virus in a dog in Sweden. J Clin Microbiol 2013, 51, 2802–2803.
32. Wernike K., Hoffmann B., Bréard E., Bøtner A., Ponsart C., Zientara S., Lohse L., Pozzi N., Viarouge C., Sarradin P., Leroux-Barc C., Riou M., Laloy E., Breithaupt A., Beer M.: Schmallenberg virus experimental infection of sheep. Vet Microbiol 2013, 166, 461–466.
