Prevalence of pathogens from Mollicutes class in cattle affected by respiratory diseases and molecular characteristics of Mycoplasma bovis field strains

Open access

Abstract

Introduction: Mycoplasma bovis is one of the main pathogens involved in cattle pneumonia. Other mycoplasmas have also been directly implicated in respiratory diseases in cattle. The prevalence of different Mycoplasma spp. in cattle affected by respiratory diseases and molecular characteristics of M. bovis field strains were evaluated. Material and Methods: In total, 713 nasal swabs from 73 cattle herds were tested. The uvrC gene fragment was amplified by PCR and PCR products were sequenced. PCR/DGGE and RAPD were performed. Results: It was found that 39 (5.5%) samples were positive for M. bovis in the PCR and six field strains had point nucleotide mutations. Additionally, the phylogenetic analysis of 20 M. bovis field strains tested with RAPD showed two distinct groups of M. bovis strains sharing only 3.8% similarity. PCR/DGGE analysis demonstrated the presence of bacteria belonging to the Mollicutes class in 79.1% of DNA isolates. The isolates were identified as: Mycoplasma bovirhinis, M. dispar, M. bovis, M. canis, M. arginini, M. canadense, M. bovoculi, M. alkalescens, and Ureaplasma diversum. Conclusion: Different Mycoplasma spp. strains play a crucial role in inducing respiratory diseases in cattle.

1. Akwuobu C.A., Ayling R.D., Chah K.F., Oboegbulem S.I.: Studies into the prevalence of Mycoplasma species in small ruminants in Benue State, North-central Nigeria. Trop Anim Health Prod 2014, 46, 1087-1092, doi 10.1007/s11250-014-0613-6.

2. Amram E., Freed M., Khateb N., Mikula I., Blum S., Spergser J., Sharir B., Ozeri R., Harrus S., Lysnyansky I.: Multiple locus variable number tandem repeat analysis of Mycoplasma bovis isolated from local and imported cattle. Vet J 2013, 197, 286-290, doi:

3. Butler J.A., Pinnow C.C., Thomson J.U., Levisohn S., Rosenbush R.F.: Use of arbitrarily primed polymerase chain reaction to investigate Mycoplasma bovis outbreaks. Vet Microbiol 2001, 78, 175-181.

4. Byrne W., McCormack M., Hotzel H., Sachse K.: Variable surface protein expression profiles and PCR fingerprints of clinical isolates of Mycoplasma bovis from Irish cattle. In: Mycoplasmas of ruminants: pathogenicity, diagnostics, epidemiology and molecular genetics, vol. 5, Edited by Poveda B., Fernandez A., Johansson K.E., Frey J. European Commission, Brussels, Belgium 2000, pp. 14-17.

5. Chazel M., Tardy F., Le Grand D., Calavas D, Poumarat F.: Mycoplasmoses of ruminants in France: recent data from the national surveillance network. BMC Vet Res 2010, 6, 32.

6. Dudek K., Bednarek D., Ayling R.D., Kycko A., Szacawa E., Karpińska T.A.: An experimental vaccine composed of two adjuvants gives protection against Mycoplasma bovis in calves. Vaccine 2016, 34, 3051-3058.

7. Dudek K., Bednarek D., Szacawa E., Rosales R.S., Ayling R.D.: Flow cytometry follow-up analysis of peripheral blood leukocyte subpopulations in calves experimentally infected with field isolates of Mycoplasma bovis. Acta Vet Hung 2015, 63, 167-178, doi:

8. Ellis J.A.: The immunology of the bovine respiratory disease complex. Vet Clin North Am Food Anim Pract 2001, 17, 535-549.

9. Hirose K., Kobayashi H., Ito N., Kawasaki Y., Zako M., Kotani K., Ogawa H., Sato H.: Isolation of mycoplasmas from nasal swabs of calves affected with respiratory diseases and antimicrobial susceptibility of their isolates. J Vet Med 2003, 347-351.

10. Hotzel H., Schneider B., Sachse K.: Investigation of Mycoplasma bovis field isolates using PCR fingerprint. In: Mycoplasma of ruminants: pathogenicity, diagnostics, epidemiology and molecular genetics, vol. 2, edited by Leori G., Santini F., Scanziani E., Frey J. European Commission, Brussels, Belgium 1998, pp. 17-19.

11. Jaccard P.: Nouvelles recherches sur la distribution florale. Bull Soc Vaudoise Sci Nat 1908, 44, 223-270.

12. Jozefová J., Ayling R.D., Zendulkowá D.: Report on investigations into the Mycoplasma species present in some clinically sick cattle in Czech Republic. Med Weter 2014, 70, 413-416.

13. Kokotovic B., Friis N.F., Ahrens P.: Mycoplasma alkalescens demonstrated in the bronchoalveolar lavage of cattle in Denmark. Acta Vet Scand 2007, 49, 2, doi:

14. Lysnyansky I., Freed M., Rosales R.S., Mikula I., Khateb N., Gerchman I., van Straten M., Levisohn S.: An overview of Mycoplasma bovis mastitis in Israel (2004-2014). Vet J 2016, 207, 180-183, doi:

15. Manso-Silván L., Dupuy V., Lysnyansky I., Ozdemir U., Thiaucourt F.: Phylogeny and molecular typing of Mycoplasma agalactiae and Mycoplasma bovis by multilocus sequencing. Vet Microbiol 2012, 161, 104-112, doi:

16. McAuliffe L., Ellis R.J., Lawes J.R., Ayling R.D., Nicholas R.A.J.: 16S rDNA PCR and denaturing gradient gel electrophoresis; a single generic test for detecting and differentiating Mycoplasma species. J Med Microbiol 2005, 54, 731-739.

17. McAuliffe L., Kokotovic B., Ayling R.D., Nicholas R.A.J.: Molecular epidemiological analysis of Mycoplasma bovis isolates from the United Kingdom shows two genetically distinct clusters. J Clin Microbiol 2004, 42, 4556-4565.

18. Nicholas R., Ayling R., McAuliffe L.: Mycoplasma Diseases of Ruminants. CABI, Wallingford, Oxfordshire, UK, 2008.

19. Nicholas R., Baker S.: Recovery of mycoplasmas from animals. In: Mycoplasma Protocols, edited by Miles R.J., Nicholas R.A.J. Humana Press, Totowa, USA, 1998, pp. 37-43.

20. Nicholas R.A.J., Ayling R.D., Stipkovits L.P.: An experimental vaccine for calf pneumonia caused by Mycoplasma bovis: clinical, cultural, serological and pathological findings. Vaccine 2002, 20, 3569-3575.

21. Nicholas R.A.J., Ayling R.D.: Mycoplasma bovis: disease, diagnosis and control. Res Vet Sci 2003, 74, 105-112.

22. Resolution No. 22/2006 of the National Ethics Committee for Experimentation of November 7, 2006, Poland.

23. Rosales R.S., Churchward C.P., Schnee C., Sachse K., Lysnyansky I., Catania S., Iob L., Ayling R., Nicholas R.A.J.: Global MLST analysis of Mycoplasma bovis isolates reveals two main population clusters J Clin Microbiol 2015, 53, 789-794.

24. Rosengarten R., Behrens A., Stetefeld A., Heller M., Ahrens M., Sachse K., Yogev D., Kirchhoff H.: Antigen heterogeneity among isolates of Mycoplasma bovis is generated by highfrequency variation of diverse membrane surface proteins. Infect Immun 1994, 62, 5066-5074.

25. Ross R.F.: Mycoplasma - animal pathogens. In: Rapid diagnosis of mycoplasmas, edited by Kahane I., Adoni A. Plenum Press, New York, 1993, pp. 69-109.

26. Schnee C., Heller M., Schubert E., Sachse K.: Point prevalence of infection with Mycoplasma bovoculi and Moraxella spp. in cattle at different stages of infectious bovine keratoconjunctivitis. Vet J 2015, 203, 92-96, doi:

27. Sneath P.H.A., Sokal R.R.: Numerical taxonomy. The principle and practice of numerical classification. WH Freeman and Co., San Francisco, 1973.

28. Subramaniam S., Bergonier D., Poumarat F., Capaul S., Schlatter Y., Nicolet J., Frey J.: Species identification of Mycoplasma bovis and Mycoplasma agalactiae based on the uvrC genes by PCR. Mol Cell Probes 1998, 12, 161-169.

29. Sulyok K.M., Kreizinger Z., Fekete L., Jánosi S., Schweitzer N., Turcsányi I., Makrai L., Erdélyi K., Gyuranecz M.: Phylogeny of Mycoplasma bovis isolates from Hungary based on multi locus sequence typing and multiple-locus variable-number tandem repeat analysis. BMC Vet Res 2014, 7, 108, doi:

30. Thomas A., Ball H., Dizier I., Trolin A., Bell C., Mainil J., Linden A.: Isolation of Mycoplasma species from the lower respiratory tract of healthy cattle and cattle with respiratory disease in Belgium. Vet Rec 2002, 151, 472-476.

Journal of Veterinary Research

formerly Bulletin of the Veterinary Institute in Pulawy

Journal Information


IMPACT FACTOR J Vet Res 2018: 0,829
5-year IMPACT FACTOR: 0,938

CiteScore 2018: 0.68

SCImago Journal Rank (SJR) 2018: 0.291
Source Normalized Impact per Paper (SNIP) 2018: 0.501

Cited By

Metrics

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 257 191 25
PDF Downloads 134 110 12