Introduction: In the present study, some of the commercial fish farms located in the Black Sea region of Turkey, were screened for bacteria between 2012 and 2014.
Material and Methods: The bacterial agents isolated from fish were identified by classical biochemical tests and the rapid diagnostic tests (API 20 E and API 20 Strep). All strains were further identified by sequencing of the 16S rRNA genes. The strains were also investigated for resistance to different antimicrobials by the disc diffusion method. Antibiotic resistance genes, including tetracycline (B), β-lactam (ampC, blaTEM, blaPSE), florfenicol (floR), erythromycine (ereA, ereB), sulphonamide (sulI, sulII), and trimethoprim (dhfr1) genes, were determined by the PCR method.
Results:Vibrio anguillarum, Vibrio fluvialis, Photobacterium damselae subsp. piscicida, Pseudomonas luteola, Lactococcus garvieae, Streptococcus iniae, Aeromonas hydrophila, and Yersinia ruckeri were isolated from marine and freshwater cultured fish. According to the results of disc diffusion, all isolates were sensitive to florfenicol, trimethoprim+sulfamethoxazole, oxitetracycline, and enrofloxacin, and resistant to lincomycin, penicillin G, and amoxicillin. Also, sulI, sulII, and floR resistance genes were detected in the bacteria.
Conclusion: The results of the study open up the opportunity to perform further investigations which could determine the possible role of ARGs in fish pathogens.
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1. Aoki T.: Present and future problems concerning the development of resistance in aquaculture. In: Chemotherapy in aquaculture: from theory to reality. Edited by Michel C. and Alderman D. Office International des Epizooties Paris 1992 pp. 254–262.
2. Arthur M. Autissier D. Courvalin P.: Analysis of the nucleotide sequence of the ereB gene encoding the erythromycin esterase type II. Nucleic Acids Res 1986 14 4987–4999.
3. Austin B. Austin D.A.: Bacterial Fish Pathogens Disease in Farmed and Wild Fish Praxis Publishing Chichester UK 2007 p. 533.
4. Balta F. Sandalli C. Kayis S. Ozgumus O.B.: Molecular analysis of antimicrobial resistance in Yersinia ruckeri strains isolated from rainbow trout (Oncorhynchus mykiss) grown in commercial fish farms in Turkey. Bull Eur Assoc Fish Path 2010 30 211–219.
5. Boran H. Terzi E. Altinok I. Capkin E. Bascinar N.: Bacterial diseases of cultured Mediterranean horse mackerel (Trachurus mediterraneus) in sea cages. Aquaculture 2013 8 396–399.
6. Capkin E. Terzi E. Altinok I.: Occurrence of antibiotic resistance genes in culturable bacteria isolated from Turkish trout farms and their local aquatic environment. Dis Aquat Org 2015 114 127–137.
8. Hall T.A.: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT Nucl Acids Symp Ser 1999 41 95–98.
9. Kayis S. Capkin E. Balta F. Altinok I.: Bacteria in rainbow trout (Oncorhynchus mykiss) in the southern Black Sea Region of Turkey - A survey. Isr J Aquacult-Bamid 2009 61 339–344.
10. Kerrn M.B. Klemmensen T.F. Moller N. Espersen F.: Susceptibility of Danish Escherichia coli strains isolated from urinary tract infections and bacteraemia and distribution of sul genes conferring sulphonamide resistance. J Antimicrob Chemother 2002 50 513–516.
11. Krumperman P.H.: Multiple antibiotic resistance indexin of Escherichia coli to identify high-risk sources of fecal contamination of foods. Appl Environ Microbiol 1983 46 165–170.
12. Kusuda R. Kawai K.: Bacterial diseases of cultured marine fish in Japan. Fish Pathol 1998 33 221–227.
13. Levy S.B. Marshall B.: Antibacterial resistance worldwide: causes challenges and responses. Nat Med 2004 10 122−129.
14. Li J. Yie J. Foo R.W.T. Ling J.M.L. Xu H. Woo N.Y.S.: Antibiotic resistance and plasmid profiles of Vibrio isolates from cultured silver sea bream Sparus sarpa. Mar Pollut Bull 1999 39 245–249.
15. Marchesi J.: Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S rRNA. Appl Environ Microbiol 1998 64 795–799.
16. Ng L.K. Martin I. Alfa M. Mulvey M.: Multiplex PCR for the detection of tetracycline resistant genes. Mol Cell Probe 2001 15 209–215.
17. Olesen I. Hasman H. Aarestrup F.M.: Prevalence of β-lactamases among ampicillin-resistant Escherichia coli and Salmonella isolated from food animals in Denmark. Microb Drug Resist 2004 10 334–340.
18. Ounissi H. Courvalin P.: Nucleotide sequence of the gene ereA encoding the erythromycin esterase in Escherichia coli. Gene 1985 35 271–278.
19. Oztürk R.C. Altinok I.: Bacterial and viral fish diseases in Turkey. Turk J Fish Aquat Sc 2014 14 275–297.
20. Schmidt A.S. Bruun M.S. Larsen J.L. Dalsgaard I.: Characterization of class 1 integrons associated with R-plasmids in clinical Aeromonas salmonicida isolates from various geographical areas. J Antimicrob Chemother 2001 47 735–743.
21. Schwartz T. Kohnen W. Jansen B. Obst U.: Detection of antibiotic-resistant bacteria and their resistance genes in waste water surface water and drinking water biofilms. FEMS Microbiol Ecol 2003 43 325–335.
22. Su H.C. Ying G.G. Tao R. Zhang R.Q. Fogarty L.R.: Occurrence of antibiotic resistance and characterization of resistance genes and integrons in Enterobacteriaceae isolated from integrated fish Farms in South China J Environ Monit 2011 13 3229–3236.
23. Toranzo A.E. Magarinos B. Romalde J.L.: A review of the main bacterial fish diseases in mariculture systems. Aquaculture 2005 246 37–61.
24. TUIK.: Turkish Statistical Institute http://www.turkstat.gov.tr/Start.do 2014 Access date: 04.06.2015.
25. Ture M. Boran H.: Phenotypic and genotypic antimicrobial resistance of Lactococcus sp. strains isolated from rainbow trout (Oncorhynchus mykiss). Bull Vet Inst Pulawy 2015 59 37–42.
26. Van T.T.H. Chin J. Chapman T. Tran L.T. Coloe P.J.: Safety of raw meat and shellfish in Vietnam: an analysis of Escherichia coli isolations for antibiotic resistance and virulence genes. Int J Food Microbiol 2008 124 217–223.
27. Zhang X.X. Zhang T. Fang H.H.P.: Antibiotic resistance genes in water environment. Appl Microbiol Biotechnol 2009 82 397−414.