Mouse duodenum as a model of inflammation induced by enterotoxigenic Escherichia coli K88

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Introduction: The aim of the experiment was to establish the enterotoxigenic Escherichia coli K88 (ETEC K88)-induced BALB/c mouse duodenum inflammation model. Material and Methods: Mice were administered different concentrations of E. coli K88 (1.0 × 107-109 CFU/mL) for 3 d by means of an esophageal catheter. Results: The results showed that the treated group expressed several significant clinical symptoms, such as reduced dietary demands and weight loss, an increased presence of IL-1α, TNF-α, and MPO in the peripheral blood, and some pathological changes in the duodenum. On the 6th-8th days, the body weight of the mice was the lowest. On the 8th day, there were significant differences in IL-1α, TNF-α, and MPO levels compared to the control group (P < 0.05), the gap between the duodenum mucous layer and the muscular layer had widened, the number of goblet cells was increased, and the inflammatory infiltrate and inflammation changes in the lamina propria and the mucous layer were the most obvious. Conclusion: The duodenum inflammation was the most severe on day 8; thus, the model was successfully established. In addition, varying concentrations of ETEC K88 did not significantly influence the duodenum inflammation (P > 0.05).

1. Cristina W.C., Donal O.T., Naomi S.T., Donald P.K., Hong L.: Are rabbits a suitable model to study sheep-associated malignant catarrhal fever in susceptible hosts? Vet Microbiol 2013, 163, 358-363.

2. Chen Q., Shen H., Yu S.Y.: Experimental study on the pathogenicity of enterotoxigenic Escherichia coli in guinea pig. Chinese J Zoonoses 2000, 16, 48-51.

3. Fairbrother J.M., Nadeau E., Gyles C.L.: Escherichia coli in postweaning diarrhea in pigs: an update on bacterial types, pathogenesis, and prevention strategies. Anim Health Res Rev 2005, 6, 17-39.

4. Julian R.M., Takuichi S., Andrew J.W., Tracey A.M., John C.F., Sarah J.H., William G.W.: Design and evalution of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S rRNA. Appl Environ Microb 1998, 64, 795-799.

5. Kerry K., Cooper J., Glenn S.: Virulence of Clostridium perfringens in an experimental model of poultry necrotic enteritis. Vet Microbiol 2010, 142, 323-328.

6. Kwang-il J., Malabi M., Venkatesan J., Shoshana B., Saul T.: Evaluation of virulent and live Shigella sonnei vaccine candidates in a gnotobiotic piglet model. Vaccine 2013, 31, 4039-4046.

7. Liu Y., Sui L.H., Zeng L.: Establishment of a mouse model of Escherichia coli O127 infection and real-time PCR detection of TGF-β1 expression. Acta Lab Anim Sci Sin 2013, 21, 13-16.

8. Mizoguchi A.: Animal models of inflammatory bowel disease. Prog Mol Biol Transl Sci 2012, 105, 263-320.

9. Mohammad F.R., Labis B., Boutigue M.M.: Catestatin decreases macrophage function in two mouse models of experimental colitis. Biochem Pharmacol 2014, 89, 386-398.

10. Nemec A., Pavlica Z., Crossley D.A., Zdovc I., Erzen D., Petelin M., Nemec M., Šentjurc M.: Single oral inoculation with Escherichia coli (ATCC 25922) stimulates generalised production of nitric oxide in mice. Acta Vet Hung 2009, 1, 127-138.

11. Randolph M.M., Allen K.P., Fleckenstein J.M.: Importance of heat-labile enterotoxin in colonization of the adult mouse small intestine by human enterotoxigenic Escherichia coli strains. Infect Immun 2006, 74, 869-875.

12. Sha T., Igaki K., Yamasaki M., Watanabe T., Tsuchimori N.: Establishment and validation of a new semi-chronic dextran sulfate sodium-induced model of colitis in mice. Int Immunopharmacol 2013, 15, 23-29.

13. Vassilis V., Giorgos B., George K.: Experimental colitis models: Insights into the pathogenesis of inflammatory bowel disease and translational issues. Eur J Pharmacol 2015, 759, 253-264.

14. Yu H., Wang L., He J.: Construction of Lactobacillus pentose expressing porcine lactoferrin and evaluation of the immune effect in mice against E. coli K88 challenge. Chinese J Prevent Vet Med 2014, 36, 889-893.

Journal of Veterinary Research

formerly Bulletin of the Veterinary Institute in Pulawy

Journal Information

IMPACT FACTOR Bull Vet Inst Pulawy 2016: 0.462

CiteScore 2016: 0.46

SCImago Journal Rank (SJR) 2015: 0.230
Source Normalized Impact per Paper (SNIP) 2015: 0.383

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