The agricultural based economy is a core business in Thailand and food export is one of the main sources of income for the Thai population. However, pesticides are overused and misused. As a result there is an urgent need to reduce the use of synthetic chemicals. Biological control offers an alternative to the use of pesticides. Mango (Mangifera indica L.) is widely planted in Thailand and is one of the major cash crops for international export. However, mango suffers from various diseases especially anthracnose, a fungal disease caused by Colletotrichum gloeosporioides. One hundred and twelve isolates of epiphytic microbes were isolated from healthy leaves and fruits of mangoes; this included 93 and 19 isolates of epiphytic bacteria and yeasts, respectively. They were screened for bioactivity against a pathogenic strain of C. gloeosporioides isolated from diseased mangoes using a dual culture technique. Out of 112 isolates, eight isolates exhibited at least 60% inhibition. These isolates were further screened for their inhibition on mango using fruit inoculation. Two isolates reduced the lesion sizes caused by C. gloeosporioides compared to control treatment. These two isolates, based on phenotypical and biochemical tests, were identified as Bacillus sp. MB61 and Bacillus sp. LB72.
Admasu W., Sahile S., Kibret M. 2014. Assessment of potential antagonists for anthracnose (Colletotrichum gloeosporioides) disease of mango (Mangifera indica L.) in North Western Ethiopia (Pawe). Archives of Phytopathology and Plant Protection 47 (18): 2176–2186.
Alamri S., Hashem M., Mostafa Y.S. 2012. In vitro and in vivo biocontrol of soil-borne phytopathogenic fungi by certain bioagents and their possible mode of action. Biocontrol Science 17 (4): 155–167.
Amin A., Khan M.A., Ehsanullah M., Haroon U., Azam S.M., Hameed A. 2012. Production of peptide antibiotics by Bacillus sp. GU 057 indigenously isolated from saline soil. Brazilian Journal of Microbiology 43: 1340–1346.
Arzanlou M., Bakhshi M., Karimi K., Torbati M. 2015. Multigene phylogeny reveals three new records of Colletotrichum spp. and several new host records for the mycobiota of Iran. Journal of Plant Protection Research 55 (2): 198–211.
Bale J.S., van Lenteren J.C., Bigler F. 2008. Biological control and sustainable food production. Philosophical Transactions of the Royal Society B: Biological Sciences 363 (1492): 761–776.
Bautista-Rosales P.U., Calderon-Santoyo M., Servín-Villegas R., Ochoa-Álvarez N.A., Vázquez-Juárez R., Ragazzo-Sánchez J.A. 2014. Biocontrol action mechanisms of Cryptococcus laurentii on Colletotrichum gloeosporioides on mango. Crop Protection 65 (1): 194–201.
Holb I.J. 2009. Fungal disease management in environmentally friendly apple production – A Review. p. 219–292. In: “Climate Change, Intercropping, Pest Control and Beneficial Microorganisms” (Lichtfouse E., ed.). Springer, The Netherlands, 514 pp.
Jacobsen B.J., Zidack N.K., Larson B.J. 2004. The role of Bacillus-based biological control agents in integrated pest management systems: Plant diseases. Phytopathology 94 (11): 1272–1275.
Jamalizadeh M., Etebarian H., Aminian H., Alizadeh A. 2009. Evaluation of Bacillus spp. as potential biocontrol agent for postharvest gray mold control on golden delicious apple in Iran. Journal of Plant Protection Research 49 (4): 405–410.
Janisiewicz W.J., Conway W.S. 2010. Combining biological control with physical and chemical treatments to control fruit decay after harvest. Stewart Postharvest Review 6 (1): 1–16.
Janisiewicz W.J., Jurick Ii W.M., Vico I., Peter K.A., Buyer J.S. 2013. Culturable bacteria from plum fruit surfaces and their potential for controlling brown rot after harvest. Postharvest Biology and Technology 76: 145–151.
Janisiewicz W.J., Korsten L. 2002. Biological control of postharvest diseases of fruits. Annual Review of Phytopathology 40 (1): 411–441.
Janisiewicz W.J., Kurtzman C.P., Buyer J.S. 2010. Yeasts associated with nectarines and their potential for biological control of brown rot. Yeast 27 (7): 389–398.
Janisiewicz W.J., Leverentz B., Conway W.S., Saftner R.A., Reed A.N., Camp M.J. 2003. Control of bitter rot and blue mold of apples by integrating heat and antagonist treatments on 1-MCP treated fruit stored under controlled atmosphere conditions. Postharvest Biology and Technology 29 (2): 129–143.
Khiyami M.A., Omar M.R., Abd-Elsalam K.A., El-Hady Aly A.A. 2014. Bacillus-based biological control of cotton seedling disease complex. Journal of Plant Protection Research 54 (4): 340–348.
Kongtragoul P., Nalumpang S., Miyamoto Y., Izumi Y., Akimitsu K. 2011. Mutation at codon 198 of Tub2 gene for carbendazim resistance in Colletotrichum gloeosporioides causing mango anthracnose in Thailand. Journal of Plant Protection Research 51 (4): 377–384.
Koomen I., Jeffries P. 1993. Effects of antagonistic microorganisms on the post-harvest development of Colletotrichum gloeosporioides on mango. Plant Pathology 42 (2): 230–237.
Lima J.R., Gondim D.M.F., Oliveira J.T.A., Oliveira F.S.A., Gonçalves L.R.B., Viana F.M.P. 2013. Use of killer yeast in the management of postharvest papaya anthracnose. Postharvest Biology and Technology 83 (1): 58–64.
Logan N.A., De Vos P. 2009 Bacillus. p. 21–128. In: “Bergey’s Manual of Systematic Bacteriology: The Firmicutes” (P. de Vos, G. Garrity, D. Jones, N.R. Krieg, W. Ludwig, F.A. Rainey, K.H. Schleifer, W.B. Whitman, eds.). Springer-Verlag, New York, USA, 1450 pp.
McGrath M., Andrews J. 2005. What an epiphyte can teach us about foliar biocontrol: Aureobasidium pullulans on apple leaves as a model system. Phytopathology 95 (6): S68–S68.
Mochizuki M., Yamamoto S., Aoki Y., Suzuki S. 2012. Isolation and characterisation of Bacillus amyloliquefaciens S13-3 as a biological control agent for anthracnose caused by Colletotrichum gloeosporioides. Biocontrol Science and Technology 22 (6): 697–709.
Mohammadipour M., Mousivand M., Jouzani G.S., Abbasalizadeh S. 2009. Molecular and biochemical characterization of Iranian surfactin-producing Bacillus subtilis isolates and evaluation of their biocontrol potential against Aspergillus flavus and Colletotrichum gloeosporioides. Canadian Journal of Microbiology 55 (4): 395–404.
Palaniyandi S.A., Yang S.H., Suh J.W. 2013. Extracellular proteases from Streptomyces phaeopurpureus ExPro138 inhibit spore adhesion, germination and appressorium formation in Colletotrichum coccodes. Journal of Applied Microbiology 115 (1): 207–217.
Panuwet P., Prapamontol T., Chantara S., Thavornyuthikarn P., Montesano M.A., Whitehead Jr R.D., Barr D.B. 2008. Concentrations of urinary pesticide metabolites in small-scale farmers in Chiang Mai Province, Thailand. Science of the Total Environment 407 (1): 655–668.
Panuwet P., Siriwong W., Prapamontol T., Ryan P.B., Fiedler N., Robson M.G., Barr D.B. 2012. Agricultural pesticide management in Thailand: Situation and population health risk. Environmental Science and Policy 17 (1): 72–81.
Peeran M.F., Krishnan N., Thangamani P.R., Gandhi K., Thiruvengadam R., Kuppusamy P. 2014. Development and evaluation of water-in-oil formulation of Pseudomonas fluorescens (FP7) against Colletotrichum musae incitant of anthracnose disease in banana. European Journal of Plant Pathology 138 (1): 167–180.
Ruangwong O.U., Chang C.I., Lamine S.A., Liang W.J. 2012. Identification of antifungal compound produced by Bacillus subtilis LB5 with ability to control anthracnose disease caused by Colletotrichum gloeosporioides. African Journal of Microbiology Research 6 (16): 3732–3738.
Rungjindamai N., Xu X.-M., Jeffries P. 2013. Identification and characterisation of new microbial antagonists for biocontrol of Monilinia laxa, the causal agent of brown rot on stone fruit. Agronomy 3 (4): 685–703.
Santos-Villalobos S. de los, Guzmán-Ortiz D.A., Gómez-Lim M.A., Délano-Frier J.P., de-Folter S., Sánchez-García P., Peña-Cabriales J.J. 2013. Potential use of Trichoderma asperellum (Samuels, Liechfeldt et Nirenberg) T8a as a biological control agent against anthracnose in mango (Mangifera indica L.). Biological Control 64 (1): 37–44.
Senghor A.L., Liang W.J., Ho W.C. 2007. Integrated control of Colletotrichum gloeosporioides on mango fruit in Taiwan by the combination of Bacillus subtilis and fruit bagging. Biocontrol Science and Technology 17 (8): 865–870.
Sharma R.R., Singh D., Singh R. 2009. Biological control of postharvest diseases of fruits and vegetables by microbial antagonists: A Review. Biological Control 50 (3): 205–221.
Soares A.C.F., Sousa C.D., Garrido M.D., Perez J.O., de Almeida N.S. 2006. Soil streptomycetes with in vitro activity against the yam pathogens Curvularia eragrostides and Colletotrichum gloeosporioides. Brazilian Journal of Microbiology 37 (4): 456–461.
Stewart A. 2001. Commercial biocontrol – reality or fantasy? Australasian Plant Pathology 30 (2): 127–131.
Traquair J.A., Singh B., Sabaratnam S. 2013. Streptomyces biocontrol of thielaviopsis and Colletotrichum root rot in tomato transplants and Fusarium fruit rot in sweet bell pepper in greenhouses. Canadian Journal of Plant Pathology 35 (1): 127–128.
Valero M., Garcia-Martinez S., Giner M.J., Alonso A., Ruiz J.J. 2010. Benomyl sensitivity assays and species-specific PCR reactions highlight association of two Colletotrichum gloeosporioides types and C. acutatum with rumple disease on Primofiori lemons. European Journal of Plant Pathology 127 (3): 399–405.
Zheng M., Shi J., Shi J., Wang Q., Li Y. 2013. Antimicrobial effects of volatiles produced by two antagonistic Bacillus strains on the anthracnose pathogen in postharvest mangos. Biological Control 65 (2): 200–206.