Fenton reagent and titanium dioxide nanoparticles as antifungal agents to control leaf spot of sugar beet under field conditions

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Abstract

In this study, foliar sprays of Fenton solutions (Fenton reaction, Fenton-like reaction and Fenton complex), titanium dioxide (TiO2) and the recommended fungicide (chlorothalonil) were estimated in the control of sugar beet leaf spot caused by Cercospora beticola under field conditions in two growing seasons. In addition, the impacts of these treatments on some crop characters (leaf dry weight, root fresh weight, soluble solid content, sucrose content and purity of sugar) were examined. Biochemical and histological changes in the livers and kidneys of treated rats compared to an untreated control were utilized to assess the toxicity of the examined curative agents. Overall, chlorothalonil and Fenton complex were the most effective treatments for disease suppression in both tested seasons followed by Fenton-like reagent, Fenton’s reagent and TiO2, respectively. Growth and yield characters of treated sugar beet significantly increased in comparison to an untreated control. There were mild or no (biochemical and histological) changes in the livers and kidneys of treated rats compared to the control. Fenton solutions and TiO2 may offer a new alternative for leaf spot control in sugar beet.

Apostol I., Heinstein P.F., Low P.S. 1989. Rapid stimulation of an oxidative burst during elicitation of cultured plant cells. Role in defense and signal transduction. Plant Physiology 90 (1): 109–116.

Arakaki T., Faust B.C. 1998. Sources, sinks, and mechanisms of hydroxyl radical (˙OH) photoproduction and consumption in authentic acidic continental cloud waters from Whiteface Mountain, New York: The role of the Fe(r) (r = II, III) photochemical cycle. Journal Geophysical Research Atmospheres 103 (3): 3487–3504.

Bancroft J.D., Stevens A. 1996. Theory and Practice of Histological Techniques. 4th ed. Churchill Livingstone, Edinburgh, UK, 766 pp.

Barham D., Trinder P. 1972. An improved colour reagent for the determination of blood glucose by the oxidase system. Analyst 97 (1151): 142–145.

Blake D.M., Maness P.C., Huang Z., Wolfrum E.J., Huang J., Jacoby W.A. 1999. Application of the photocatalytic chemistry of titanium dioxide to disinfection and the killing of cancer cells. Separation and Purification Reviews 28 (1): 1–50.

Carruthers A., Oldfield J.F.T. 1961. Methods for the assessment of beet quality. International Sugar Journal 63: 103–105.

Duncan D.B. 1955. Multiple ranges and multiple F test. Biometrics 11 (1): 1–42.

Eweis M., Elkholy S.S., Elsabee M.Z. 2006. Antifungal efficacy of chitosan and its thiourea derivatives upon the growth of some sugar-beet pathogens. International Journal of Biological Macromolecules 38 (1): 1–8.

Faust B.C., Zepp R.G. 1993. Photochemistry of aqueous iron (III) – polycarboxylate complexes: roles in the chemistry of atmospheric and surface waters. Environmental Science Technology 27 (12): 2517–2522.

Frazer L. 2001. Titanium dioxide: environmental white knight. Environmental Health Perspective 109 (4): 174–177.

Hammel K.E., Kapich A.N., Jensen Jr. K.A., Ryan Z.C. 2002. Reactive oxygen species as agents of wood decay by fungi. Enzyme and Microbial Technology 30 (4): 445–453.

Hamza A.M., Mohamed A.A.A., Derbalah A.S. 2015. Recent trends in bio-controlling of late blight pathogen in tomato under field conditions. Egyptian Journal of Biological Pest Control 25 (1): 145–151.

Hamza A.M., Mohamed A.A.A., Hamed S. 2016. New trends for biological and non-biological control of tomato root rot, caused by Fusarium solani, under greenhouse conditions. Egyptian Journal of Biological Pest Control 26 (1): 89–96.

Helrich K. 1990. Official Methods of Analysis of the Association of Official Analytical Chemists. Vol. I. Association of Official Analytical Chemists, Arlington, Virginia, USA, 771 pp.

Holtschulte B. 2000. Cercospora beticola – worldwide distribution and incidence. p. 5–16. In:“Cercospora beticola Sacc. Biology, Agronomic Influence and Control Measures in Sugar Beet.” Vol. 2. (M.J.C. Asher, B. Holtschulte, M.R. Molard, F. Rosso, G. Steinrücken, R. Beckers, eds.). International Institute for Beet Research, Brussels, Belgium, 215 pp.

Imlay J.A., Chin S.M., Linn S. 1988. Toxic DNA damage by hydrogen peroxide trough the Fenton reaction in vivo and in vitro. Science 240 (4852): 640–642.

Lamey H.A., Cattanach A.W., Bugbee W.M. 1987. Cercospora leaf spot of sugarbeet. North Dakota State University Extension Circular PP-764 Revised, 4 pp.

Lamey H.A., Cattanach A.W., Bugbee W.M., Windels C.E. 1996. Cercospora leaf spot of sugar beet. North Dakota State University Extension Circular PP-764 Revised, 4 pp.

Makowski A., Wardas W. 2001. Photocatalytic degradation of toxins secreted to water by cyanobacteria and unicellular algae and photocatalytic degradation of the cells of selected microorganisms. Current Topics in Biophysics 25 (1): 19–25.

Maness P.C., Smolinski S., Blake D.M., Huang Z., Wolfrum E.J., Jacoby W.A. 1999. Bactericidal activity of photocatalytic TiO2 reaction: toward an understanding of its killing mechanism. Applied and Environmental Microbiology 65 (9): 4094–4098.

Matsunaga T., Tomoda R., Nakajima T., Nakamura N., Komine T. 1988. Continuous-sterilization system that uses photosemiconductor powders. Applied and Environmental Microbiology 54 (6): 1330–1333.

May R.M. 1985. Evolution of pesticide resistance. Nature 315: 12–13.

McGinnis R.A. 1982. Sugar Beet Technology. 3rd ed. Beet Sugar Development Foundation, Fort Collins, Colorado, 855 pp.

Mehdy M.C. 1994. Active oxygen species in plant defense against pathogens. Plant Physiology 105 (2): 467–472.

Oshira T., Yamamoto O., Iida Y., Nakagawa Z. 2008. Antibacterial activity of ZnO powder with crystallographic orientation. Journal of Materials Science: Materials in Medicine 19 (3): 1407–1412.

Papadakis A.K., Roubelakis-Angelakis K.A. 1999. The generation of active oxygen species differs in tobacco and grapevine mesophyll protoplasts. Plant Physiology 121 (1): 197–205.

Polo-López M.I., García-Fernández I., Oller I., Fernández-Ibáñez P. 2011. Solar disinfection of fungal spores in water aided by low concentrations of hydrogen peroxide. Photochemistry and Photobiology Science 10 (3): 381–388.

Reitman S., Frankel S. 1957. A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. American Journal of Clinical Pathology 28 (1): 56–64.

Rodríguez-Chueca J., Mosteo R., Ormad M.P., Miguel N., Ovelleiro J.L. 2011. Heterogeneous photo-Fenton processes for disinfection of treated urban wastewater. Proceedings of the 20th IOA World Congress – 6th IUVA World Congress, Ozone and UV: Leading edge science and technologies. Paris, France, 23–27 May 2011, 70 pp.

Romestaing C., Piquet M.A., Bedu E., Rouleau V., Dautresme M., Hourmand-Ollivier I.H., Filippi C., Duchamp C., Sibille B. 2007. Long term highly saturated fat diet does not induce NASH in Wistar rats. Nutrition and Metabolism 4: 4.

Sakugawa H., Hasan N., Oguntimehin I., Belal E. 2012. Protective and curative effects of foliar-spray Fenton solutions against cucumber (Cucumis sativus, L.) powdery mildew. Journal of Environmental Science and Health, Part A 47 (12): 1909–1918.

Sakugawa Y. 2008. Effects of hydroxyl radicals generated by photo-Fenton reaction on powdery mildew of strawberries. Japan Journal of Phytopathology 74 (2): 110–113.

Schopfer P. 1994. Histochemical demonstration and localization of H2O2 in organs of higher plants by tissue printing on nitrocellulose paper. Plant Physiology 104 (4): 1269–1275.

Shane W.W., Teng P.S. 1983. Sugarbeet yield losses due to Cercospora leaf spot. Sugarbeet Research and Extension Reports 14: 193–198.

Shane W.W., Teng P.S. 1992. Impact of Cercospora leaf spot on root weight, sugar yield and purity of Beta vulgaris. Plant Disease 76 (8): 812–820.

Spuhler D., Rengifo-Herrera J.A., Pulgarin C. 2010. The effect of Fe2+, Fe3+, H2O2 and the photo-Fenton reagent at near neutral pH on the solar disinfection (SODIS) at low temperatures of water containing Escherichia coli K12. Applied Catalysis B: Environmental 96 (1–2): 126–141.

Sunada K., Watanabe T., Hashimoto K. 2003. Studies on photo-killing of bacteria on TiO2 thin film. Journal of Photochemistry and Photobiology A: Chemistry 156 (1–3): 227–233.

Urech P.A., Staub T., Voss G. 1997. Resistance as a concomitant of modern crop protection. Pest Management Science 51 (3): 227–234.

Vanacker H., Carver T.L.W., Foyer C.H. 2000. Early H2O2 accumulation in mesophyll cells leads to induction of glutathione during the hyper-sensitive response on the barley-powdery mildew interaction. Plant Physiology 123 (4): 1289–1300.

Williams R.J., Heymann D.L. 1998. Containment of antibiotic resistance. Science 279 (5354): 1153–1154.

Witte W. 1998. Medical consequences of antibiotic use in agriculture. Science 279 (5353): 996–997.

Zuo Y., Hoigne J. 1992. Formation of hydrogen peroxide and depletion of oxalic acid in atmospheric water by photolysis of iron (III)-oxalate complex. Environmental Science and Technology 26 (5): 1014–1022.

Journal of Plant Protection Research

The Journal of Polish Society of Plant Protection, Committee of Plant Protection; Polish Academy of Sciences, Institute of Plant Protection – National Research Institute

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CiteScore 2016: 0.84

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