Efficacy of Three Entomopathogenic Fungi Alone or in Combination With Diatomaceous Earth Modifications for the Control of Three Pyralid Moths in Stored Grains

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Efficacy of Three Entomopathogenic Fungi Alone or in Combination With Diatomaceous Earth Modifications for the Control of Three Pyralid Moths in Stored Grains

The efficacy of Natural diatomaceous (DE) alone and combined with three fungal pathogens: Beauveria bassiana, Metarhizium anisopliae and Isaria fumosorosea against three moth species, Plodia interpunctella, Ephestia cautella and E. kuehniella was evaluated. Modified diatoms with Calcium hydroxide (Ca-DE) and modified diatoms with Sodium hydroxide (Na-DE) were the highlight treatments against tested insects and achieved the highest mortality percentages. E. kuehniella achieved the highest tolerant to tested DEs. B. bassiana was the most effective fungus alone against the three tested insects. Ca-DE and Na-DE treatments strongly enhanced the potency of the tested fungi. E. kuehniella was more susceptible to M. anisopliae and B. bassiana. Larvae of P. interpunctella and E. cautella were more tolerant to I. fumosorosea alone than E. kuehniella. In most cases, DE combinations with tested fungi had synergistic effects, while in E. cautella, modified diatoms with Aluminium hydroxide (Al-DE) decreased the efficacy of M. anisopliae and I. fumosorosea. Also, Al-DE and DE impaired the efficacy of I. fumosorosea against E. kuehniella. The egg production was highly suppressed by combination of Ca-DE followed by Na-DE with tested fungi in comparison to untreated control. The combination of Ca-DE/Beauveria bassiana strongly suppressed the number of deposited eggs of P. interpunctella (54.6±5.8 eggs/female), in comparison to untreated control (288.3±3.4 eggs/female).

The most effective DEs modification were Ca-DE and Na-DE had insecticidal, repellent and ovicidal effects against tested insects and they had synergistic effects on the potency of tested fungi.

Abbott W.S. 1925. A method of computing the effectiveness of an insecticide. J. Econ. Entomol. 18 (1): 265-267.

Abd-El-Aziz SH.E., Sherief, M.A. 2010. Insecticidal effects of modified diatomaceous earth (DE) with different hydroxides (MOH, M = Na, Ca, Al) against Callosobruchus maculatus (F.) beetles (Coleoptera: Bruchidae) on stored cowpea grains. J. Entomol. Res. 34 (1): 1-9.

Akbar W., Lord J.C., Nechols J.R., Howard R.W. 2004. Diatomaceous earth increases the efficacy of Beauveria bassiana against Tribolium castaneum larvae and increases conidia attachment. J. Econ. Entomol. 97 (2): 273-280.

Batta Y.A. 2004. Control of the rice weevil (Sitophilus oryzae L., Coleoptera: Curculionidae) with various formulations of Metarhizium anisopliae. Crop Prot. 23 (2): 103-108.

Būda V., Pečiulytė D. 2008. Pathogenicity of four fungal species to Indian meal moth Plodia interpunctella (Hübner) (Lepidoptera: Pyralidae). Ekologija 54 (4): 265-270.

Cox P.D., Bell C.H. 1991. Biology and ecology of moth pests of stored foods. p. 181-193. In: "Ecology and Management of Food Industry Pests" (J.R. Gorham, ed.). Association of Official Analytical Chemists, Arlington, VA, 595 pp.

Cox P.D., Wilking D.R. 1996. The Potential Use of Biological Control of Pests in Stored Grain. Research Review 36, Home-Grown Cereals Authority, London, 53 pp.

Ebeling W. 1971. Sorptive dust for pest control. Ann. Rev. Entomol. 16 (1): 123-158.

Fields P.G. 1999. Diatomaceous earth: Advantages and limitations. p. 781-784. In: Proc. 7th International Conference on Stored-Product Protection (Z. Jin, Q. Liang, Y. Liang, X. Tan, L. Guan, eds). Beijing, P.R. China, 14-19 October 1998. Sichuan Publishing House of Science and Technology, Chengdu, Sichuan Province, P.R. China, 783 pp.

Finney D.J. 1964. Probit Analysis. 2nd ed. Cambridge. Univ. Press, England, 318 pp.

Horak M. 1994. A review of cadra walker in Australia: five new native species and the two introduced pest species (Lepidoptera: Pyralidae: Phycitinae). Aust. J. Entomol. 33 (3): 245-262.

Kavallieratos N.G., Athanassiou C.G., Michalaki M.P., Batta Y.A., Rigatos H.A., Pashalidou F.G., Balotis G.N., Tomanovic Z., Vayias B.J. 2006. Effect of the combined use of Metarhizium anisopliae (Metschnikoff) Sorokin and diatomaceous earth for the control of three stored-product beetle species. Crop Prot. 25 (10): 1087-1094.

Michalaki M.P., Athanassiou C.G., Kavallieratos N.G., Batta Y.A., Balotis J.N. 2006. Effectiveness of Metarhizium anisopliae (Metschnikoff) Sorokin applied alone or in combination with diatomaceous earth against Tribolium confusum Jacquelin du Val: influence of temperature relative humidity and type of commodity. Crop Prot. 25 (5): 418-425.

Michalaki M.P., Athanassiou C.G., Steenberg T., Buchelos C. Th. 2007. Effect of Paecilomyces fumosoroseus (Wise) Brown and Smith (Ascomycota: Hypocreales) alone or in combination with diatomaceous earth against Tribolium confusum Jacquelin du Val (Coleoptera: Tenebrionidae) and Ephestia kuehniella Zeller (Lepidoptera: Pyralidae). Biol. Control. 40 (2): 280-286.

Moore D., Lord J.C., Smith S.M. 2000. Pathogens. p. 193-227. In: "Alternatives to Pesticides in Stored-Product IPM" (Bh. Subramanyam, D.W. Hagstrum, eds.). Kluwer Academic Publishers, Dordreecht, 193 pp.

Korunic Z., Mackay A. 2000. Grain surface-layer treatment of diatomaceous earth for insect control. Arhiv za Higijenu Rada Toksikologiju 51 (1): 1-11.

Lord J.C. 2001. Desiccant dusts synergize the effect of Beauveria bassiana (Hyphomycetes: Moniliales) on stored-grain beetles. J. Econ. Entomol. 94 (2): 367-372.

Lord J.C. 2005. Low humidity, moderate temperature and dessicant dust favor the efficacy of Beauveria bassiana (Hyphomycetes: Moniliales) for the lesser grain borer Rhyzopertha dominica (Coleoptera: Bruchidae). Biol. Control 34 (2): 180-186.

Lwande W., Hassanalli A., Njorage P.W., Bentley M.D., Delle Monache F., Jondiks J.I. 1985. A new 6a-hydroxy petrocarpan with insect antifeedant and antifungal properties from the root of Tephrosia hildebrandtu. Vatle. Ins. Sci. Appl. 6 (4): 537-541.

Sabbour M.M., Sahab A.F. 2005. Efficacy of some microbial control agents against cabbage pests in Egypt. Pak. J. Biol. Sci. 10 (8): 1351-1356.

Sabbour M.M, Abd El-Aziz, Sh, E. 2007a. Efficiency of some bioinsecticides against broad bean beetle, Bruchus rufimanus (Coleoptera: Bruchidae). Res. J. Agric. Biol. Sci. 3 (2): 67-72.

Sabbour M.M., Abd-El-Aziz Sh.E. 2007b. Evaluation of some bioinsecticides and packaging materials for protecting broad bean against Callosobruchus maculatus (Coleoptera: Bruchidae) infestation during storage. Bull. Ent. Soc. Egypt., Econ Series 33 (2): 129-137.

Sabbour M.M., Abd-El-Aziz Sh.E. 2010. Efficacy of some bioinsecticides against Bruchidius incarnates (Boh.) (Coleoptera: Bruchidae) infestation during storage. J. Plant Prot. Res. 50 (1): 25-31.

Subramanyam Bh., Roesli R. 2000. Inert dusts. p. 321-380. In: "Alternatives to Pesticides in Stored-Product IPM" (Bh. Subramanyam, D.W. Hagstrum, eds). Kluwer Academic Publishers, Dordreecht, 437 pp.

Journal of Plant Protection Research

The Journal of Polish Society of Plant Protection, Committee of Plant Protection; Polish Academy of Sciences, Institute of Plant Protection – National Research Institute

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