The Relevance of Oxidative Stress Status in One Week and One Month Alcohol Abstinent Patients

Open access

Abstract

Background: Although it is generally accepted that there is an increased oxidative stress status in alcoholics, the separate relevance of oxidative stress following alcohol withdrawal is still not understood to this date. There are reports stating that the increased oxidative stress status in alcoholics may persist independently of the constant presence of alcohol intake, while on the other side, it was demonstrated that the antioxidant defense mechanism could significantly increase after alcohol withdrawal.

Methods: In the present work, we were interested in studying the relevance of oxidative stress status in the alcohol withdrawal processes, by determining some oxidative stress markers (two antioxidant enzymes: superoxide dismutase - SOD and glutathione peroxidase - GPX and a lipid peroxidation maker - MDA) after one week and one month of abstinence, as compared to the baseline and a control group of subjects.

Results: Our data confirmed the increased oxidative stress status in alcoholic patients and, more importantly, we de m - onstrated here a significant decrease of the oxidative stress status one week and one month following the withdrawal, as showed by a significant increase in the specific activity of SOD (p<0.003), as well as by a decrease in MDA levels (p<0.019). Still, in the case of all three markers of oxidative stress status which we determined, the levels after one week or one month of abstinence were significantly altered when compared to controls.

Conclusions: This suggests that severe and prolonged deficiency in the oxidative stress marker levels needs longer than one month of abstinence to normalize.

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • 1. Seiva FR Amauchi JF Rocha KK Ebaid GX Souza G Fernandes AA et al. Alcoholism and alcohol abstinence: N-acetylcysteine to improve energy expenditure myocardial oxidative stress and energy metabolism in alcoholic heart disease. Alcohol 2009a; 43: 649-56.

  • 2. Ferreira MP Willoughby D. Alcohol consumption: the good the bad and the indifferent. Appl Physiol Nutr Metab 2008; 33: 12-20.

  • 3. Sutherland GT Sheahan PJ Matthews J Dennis CV Sheedy DS McCrossin T et al. The effects of chronic alcoholism on cell proliferation in the human brain. Exp Neurol 2013; 247: 9-18.

  • 4. Montoya ID. The pathology of alcohol use and abuse. Clin Lab Sci 2013; 26: 15-22.

  • 5. Sies H. Oxidative stress: oxidants and antioxidants. Exp Physiol 1997; 82: 291-5.

  • 6. Hamad M Awadallah S Nasr H. The relationship between haptoglobin polymorphism and oxidative stress in hemodialysis patients. J Med Biochem 2013; 32: 220-6.

  • 7. Ciobica A Hritcu L Nastasa V Padurariu M Bild W. Inhibition of central angiotensin converting enzyme exerts anxiolytic effects by decreasing brain oxidative stress. J Med Biochem 2011; 30: 109-14.

  • 8. Ciobica A Padurariu M Dobrin I Stefanescu C Dobrin R. Oxidative stress in schizophrenia - focusing on the main markers. Psychiatr Danub 2011; 23: 237-45.

  • 9. Ciobica A Olteanu Z Padurariu M Hritcu L. The effects of low-dose pergolide on memory and oxidative stress in a 6-OHDA induced rat model of Parkinson’s disease. Journal of Physiology and Biochemistry 2012; 68: 59-69.

  • 10. Stefanescu C Ciobica A. The relevance of oxidative stress status in first episode and recurrent depression. Journal of Affective Disorders 2012; 143: 34-8.

  • 11. Padurariu M Ciobica A Lefter R Serban IL Stefanescu C Chirita R. The oxidative stress hypothesis in Alzhei - mer’s disease. Psychiatr Danub 2013; 25.

  • 12. Lecomte E Herberlk B Pirollet P Chanterelle Y. Effect of alcohol consumption on blood antioxidant nutrients and oxidative stress indicators. Am J Clin Nutr 1994; 60: 255-61.

  • 13. Shaw S. Lipid peroxidation iron mobilization and radical generation induced by alcohol. Free Radic Biol Med 1989; 7: 541-7.

  • 14. Lieber CS. Biochemical and molecular basis of alcohol induced injury to liver and other tissues. N Engl J Med 1988; 319: 1639-42.

  • 15. Marotta F Reizakovic I Tajiri H Safran P Ideo G. Abstinence-induced oxidative stress in moderate drinkers is improved by bionormalizer. Hepatogastroenterology 1997; 44: 1360-6.

  • 16. Huang MC Chen CH Peng FC Tang SH Chen CC. Alterations in oxidative stress status during early alcohol withdrawal in alcoholic patients. J Formos Med Assoc 2009a; 108: 560-9.

  • 17. Guemouri L Lecomte E Herbeth B. Blood activities of antioxidant enzymes in alcoholics before and after withdrawal. J Stud Alcohol 1993; 54: 626-9.

  • 18. Tsai GE Ragan P Chang R. Increased glutamatergic neurotransmission and oxidative stress after alcohol withdrawal. Am J Psychiatry 1998; 155: 726-32.

  • 19. Huang MC Chen CC Peng FC Tang SH Chen CH. The correlation between early alcohol withdrawal severity and oxidative stress in patients with alcohol dependence. Prog Neuropsychopharmacol Biol Psychiatry 2009b; 33: 66-9.

  • 20. Peng FC Tang SH Huang MC Chen CC Kuo TL Yin SJ. Oxidative status in patients with alcohol dependence: a clinical study in Taiwan. J Toxicol Environ Health A 2005; 68: 1497-509.

  • 21. Kannan M Wang L Kang YJ. Myocardial oxidative stress and toxicity induced by acute ethanol exposure in mice. Exp Biol Med 2004; 229: 553-9.

  • 22. Yuksel N Uzbay IT Karakilic H. Increased serum nitrite/nitrate (NOx) and malondialdehyde (MDA) levels during alcohol withdrawal in alcoholic patients. Pharmaco - psychiatry 2005; 38: 95-6.

  • 23. Le Lan C Ropert M Laine F Medevielle M Jard C Pouchard M et al. Serum ceruloplasmin and ferroxidase activity are not decreased in hepatic failure related to alcoholic cirrhosis: clinical and pathophysiological implications. Alcohol Clin Exp Res 2004; 28: 775-9.

  • 24. Shaw S Jayatilleke E. Ethanol-induced iron mobilization: role of acetaldehyde-aldehyde oxidase generated superoxide. Free Radic Biol Med 1990; 9: 11-17.

  • 25. Steinberg D Pearson TA Kuller LH. Alcohol and atherosclerosis. Ann Intern Med 1991; 114: 967-76.

  • 26. Cederbaum AI. Oxygen radical generation by microsomes: role of iron and implications for alcohol metabolism and toxicity. Free Radic Biol Med 1989; 7: 559-67.

  • 27. Shaw S. Lipid peroxidation iron mobilization and radical generation induced by alcohol. Free Radic Biol Med 1989; 7: 541-7.

  • 28. Girre C Hispard E Therond P. Effect of abstinence from alcohol on the depression of glutathione peroxidase activity and selenium and vitamin E levels in chronic alcoholic patients. Alcohol Clin Exp Res 1990; 14: 909-12.

  • 29. Soardo G Donnini D Varutti R. Alcohol-induced endothelial changes are associated with oxidative stress and are rapidly reversed after withdrawal. Alcohol Clin Exp Res 2005; 29: 1889-98.

  • 30. Situnayake RD Crump BJ Thurnham DI et al. Lipid peroxidation and hepatic antioxidants in alcoholic liver disease. Gut 1990; 31: 1311-17.

  • 31. Wang T Zhu D Xu X Xu Y. The amelioration of AH by abstinence and the attenuation of oxidative stress. Hepatogastroenterology 2012; 59: 73-6.

  • 32. Gunasekar PG Kanthasamy AG Borowitz JL Isom GE. NMDA receptor activation produces concurrent generation of nitric oxide and reactive oxygen species: implication for cell death. J Neurochem 1995; 65: 2016-21.

  • 33. Bild W Ciobica A Padurariu M Bild V. The interdependence of the reactive species of oxygen nitrogen and carbon. J Physiol Biochem 2013; 69: 147-54.

  • 34. Adams ML Sewing BN Chen J Meyer ER Cicero TJ. Nitric oxide-related agents alter alcohol withdrawal in male rats. Alcohol Clin Exp Res 1995; 19: 195-9.

  • 35. Diniz YS Rocha K Souza G Galhardi C Ebaid G Novelli Filho JL et al. Effects of N-acetylcysteine on sucrose-rich diet-induced hyperglycaemia dyslipidemia and oxidative stress in rats. Eur J Pharmacol 2006; 543: 151-7.

  • 36. Ozaras R Tahan V Aydin S Uzun H Kaya S Senturk H. N-acetylcysteine attenuates alcohol-induced oxidative stress in rats. World J Gastroenterol 2003; 9 791-4.

  • 37. Seiva FR Amauchi JF Ribeiro Rocha KK Souza GA Ebaid GX Burneiko RM et al. Effects of N-acetylcysteine on alcohol abstinence and alcohol-induced adverse effects in rats. Alcohol 2009b; 43: 127-35.

  • 38. Otis JS Guidot DM. Procysteine increases alcoholdepleted glutathione stores in rat plantaris following a period of abstinence. Alcohol 2010; 45: 495-500.

Search
Journal information
Impact Factor

IMPACT FACTOR 2018: 2.000
5-year IMPACT FACTOR: 1.075

CiteScore 2018: 1.47

SCImago Journal Rank (SJR) 2018: 0.523
Source Normalized Impact per Paper (SNIP) 2018: 0.581

Cited By
Metrics
All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 251 171 2
PDF Downloads 138 107 0