Association Between Telomere Shortening and Ageing During Occupational Exposure
Telomere length is considered as a biomarker of ageing, resulting in shortening during the process. The present investigation was an attempt to determine the relative telomere length in mechanical workshop workers. Telomere length shortening in cells during occupational exposure causes accelerated ageing. Genomic DNA was isolated from buccal epithelial cells collected from 240 individuals, comprising two groups of 120 exposed workers and 120 unexposed controls. Telomere length was measured by using real time PCR. Both telomere (T) and single copy gene (S) specific primers were used to compute the relative T/S ratio and expressed as the relative telomere length. Telomere length differed significantly between the workers and controls (p<0.05). The results showed an indirect and significant association (r=-0.356, p=0.001) between age and telomere length in the workers. This study showed that the difference in telomere length shortening was statistically significant (p<0.05) between the workers and controls. It was concluded that occupational exposure acts as a risk factor to enhance telomere length shortening and accelerate ageing.
Von Zglinicki T. Oxidative stress shortens telomeres. Trends Biochem Sci 2002; 27: 339-44.
Richter T Von Zglinicki T. A continuous correlation between oxidative stress and telomere shortening in fibroblasts. Exp Gerontol 2007; 42: 1039-42.
McGrath M Wong JYY Michaud D Hunter DJ De Vivo I. Telomere Length Cigarette Smoking and Bladder Cancer Risk in Men and Women. Cancer epidemiology biomarkers & prevention: a publication of the American Association for Cancer Research cosponsored by the American Society of Preventive Oncology 2007; 16: 815-19.
Allsopp RC Vaziri H Patterson C Goldstein S Younglai EV Futcher AB et al. Telomere length predicts replicative capacity of human fibroblasts. Proceedings of the National Academy of Sciences of the United States of America 1992; 89: 10114-18.
Jennings BJ Ozanne SE Dorling MW Hales CN. Early growth determines longevity in male rats and may be related to telomere shortening in the kidney. FEBS Lett. 1999; 448: 4-8.
Haussmann MF Winkler DW O'Reilly KM Huntington CE Nisbet ICT Vleck CM. Telomeres shorten more slowly in long-lived birds and mammals than in short-lived ones. Proc R Soc B 2003; 270: 1387-92.
Herbig U Ferreira M Condel L Carey D Sedivy JM. Cellular senescence in aging primates. Science 2006; 311: 1257.
Jemielity S Kimura M Parker KM Parker JD Cao X Aviv A et al. Short telomeres in short-lived males: what are the molecular and evolutionary causes? Aging Cell 2007; 6: 225-33.
Kang MK Kameta A Baluda MA Park NH. Telomere shortening does not occur during postmaturational aging in situ in normal human oral fibroblasts. Mecha nisms of Ageing and Development 2003; 124: 873-6.
Okuda K Bardeguez A Gardner JP Rodriguez P Ganesh V Kimura M et al. Telomere length in the newborn. Pediatric Research 2002; 52: 377-81.
Youngren K Jeanclos E Aviv H Kimura M Stock J Hanna M et al. Synchrony in telomere length of the human fetus. Human Genetics 1998; 102: 640-3.
Baird D Rowson J Wynford-Thomas D Kipling D. Extensive allelic variation and ultrashort telomeres in senescent human cells. Nature Genetics 2003; 33: 203-7.
Pauliny A Wagner R Augustin J Szep T Blomqvist D. Age-independent telomere length predicts fitness in two bird species. Mol Ecol 2006; 15: 1681-7.
Haussmann MF Winkler DW Vleck CM. Longer telomeres associated with higher survival in birds. Biol Lett 2005; 1: 212-14.
Joeng KS Song EJ Lee KJ Lee J. Long lifespan in worms with long telomeric DNA. Nature Genetics 2004; 36: 607-11.
Cawthon RM Smith KR O'Brien E Sivatchenko A Kerber RA. Association between telomere length in blood and mortality in people aged 60 years or older. Lancet 2003; 361: 393-5.
Rudolph KL Chang S Lee HW Blasco M Gottlieb GJ Greider C et al. Longevity stress response and cancer in aging telomerase-deficient mice. Cell 1999; 96: 701-12.
Monaghan P Haussmann MF. Do telomere dynamics link lifestyle and lifespan? Trends Ecol Evol 2006; 21: 47-53.
Jiang WQ Zhong ZH Henson JD Reddel RR. Identification of candidate alternative lengthening of telomeres genes by methionine restriction and RNA interference. Oncogene 2007; 26: 4635-47.
Martino-Roth MG Viegas J Amaral M Oliveira L Ferreira FLS Erdtmann B. Evaluation of genotoxicity through micronuclei test in workers of car and battery repair garages. Genetics and Molecular Biology 2002; 25: 495-500.
Cawthon RM. Telomere measurement by quantitative PCR. Nucleic Acids Research 2002; 30: e47.
Hewakapuge S Van Oorschot RAH Lewandowski P Baindur-Hudson S. Investigation of telomere lengths measurement by quantitative real-time PCR to predict age. Legal Medicine 2008; 10: 236-42.
Huda N Tanaka H Herbert BS Reed T Gilley D. Shared environmental factors associated with telomere length maintenance in elderly male twins. Aging Cell 2007; 6: 709-13.
Cui Q Tang C. Effects of lead and selenium on yeast (Saccharomyces cerevisiae) telomere. Journal of Environmental Science and Health Part A 2000; 35: 1663-71.
Jacobus JA Flor S Klingelhutz AJ Robertson LW Ludewig G. 2-(4-chlorophenyl)-14-benzoquinone increases the frequency of micronuclei and shortens telomeres. Environmental Toxicology and Pharmacology 2008; 25: 267-72.
Hoxha M Dioni L Bonzini M Pesatori AC Fustinoni S Cavallo D et al. Association between leukocyte telomere shortening and exposure to traffic pollution: a cross-sectional study on traffic officers and indoor office workers. Environmental Health Perspectives 2009; 8: 41.
Nordfjall K Larefalk A Lindgren P Holmberg D Roos G. Telomere length and heredity: indications of paternal inheritance. Proceedings of the National Academy of Sciences of the United States of America 2005; 102: 16374-8.
Njajou OT Cawthon RM Damcott CM Wu SH Ott S Garant MJ et al. Telomere length is paternally inherited and is associated with parental lifespan. Proceedings of the National Academy of Sciences of the United States of America 2007; 104: 12135-9.
Graakjaer J Pascoe L Der-Sarkissian H Thomas G Kolvraa S Christensen K et al. The relative lengths of individual telomere are defined in the zygote and strictly maintained during life. Aging Cell 2004; 3: 97-102.
Slagboom PE Droog S Boomsma DI. Genetic determination of telomere size in humans: twin study of three age groups. American Journal of Human Genetics 1994; 55: 876-82.
Rufer N Brümmendorf TH Kolvraa S Bischoff C Christensen K Wadsworth L et al. Telomere fluorescence measurements in granulocytes and T lymphocyte subsets point to a high turnover of hematopoietic stem cells and memory T cells in early childhood. The Journal of Experimental Medicine 1999; 190: 157-68.
Thomas P O'Callaghan NJ Fenech M. Telomere length in white blood cells buccal cells and brain tissue and its variation with ageing and Alzheimer's disease. Mechanisms of Ageing and Development 2008; 129: 183-90.
Mooney LA Bell DA Santella RM Van Bennekum AM Ottman R Paik M et al. Contribution of genetic and nutritional factors to DNA damage in heavy smokers. Carcinogenesis 1997; 18: 503-9.
Norppa H. Cytogenetic biomarkers and genetic polymorphisms. Toxicology Letters 2004; 149: 309-34.
Pinto D Ceballos JM Garcia G Guzman P Del Razo LM Vera E et al. Increased cytogenetic damage in outdoor painters. Mutation Research 2000; 467: 105-11.
Manikantan P Balachandar V Sasikala K. DNA Damage in Workers Occupationally Exposed to Lead Using Comet Assay. International Journal of Biology 2010; 2: 103-10.
Ishikawa H Yamamoto H Tian Y Kawano M Yamauchi T Yokoyama K. Evidence of association of the CYP2E1 genetic polymorphism with micronuclei frequency in human peripheral blood. Mutation Research 2004; 546: 45-53.