Propagation in vitro of alstroemeria ligtu hybrid through direct organogenesis from leaf base

Open access


In the present study, multiplication efficiency of Alstroemeria ligtu hybrid was investigated. Bases of the first seedling leaves grown in vitro were used as initial explants. The explants were cultured in the MS media containing 3% sucrose, 0.7% agar at pH 5.8, five N6-benzylaminopurine (BAP) concentrations (0, 0.5, 1, 1.5 and 2 mg·1-1) and three indole-3-butyric acid (IBA) concentrations (0, 0.1 and 0.2 mg·1-1). The cultures were incubated at 21 ± 2 °C under photoperiod 16/8. After three subculturings (3 weeks-long each) the number of rhizome, shoots, buds, leaves, and roots, length of shoots and roots were recorded. Adventitious shoots formed directly on the leaf bases without callus intervention. Their number was affected by BAP concentrations. The highest shoots number, six per explants, was obtained at 1 mg·1-1 BAP and 0.1 mg·1-1 IBA. The shoot length decreased with the increasing concentration of BAP. The highest root number (2.7) was formed on shoots cultured on the MS medium containing 0.5 mg·1-1 α- naphthalene acetic acid, and the highest rhizome number (2.2) was formed on the medium with 0.5 mg·1-1 BAP. In vitro rooted plantlets were able to survive and acclimatize in the greenhouse.

Asghari F., Hossienil B., Hassani A., Shirzad H. 2012. Effect of explants source and different hormonal combinations on direct regeneration of basil plants (Ocimum basilicum L.). Aust. J. Agric. Eng. 3: 12-17.

Bond S., Alderson P.G. 1993. The influence of apical dominance on in vitro multiplication of the rhizome of Alstroemeria. J. Hort. Sci. 68: 905-910.

Chiari A., Bridgen M.P. 2000. Rhizome splitting: a new micropropagation technique to increase in vitro propagule yield in Alstroemeria. Plant Cell Tiss.Org. Cult. 62: 39-46.

Fay M.F., Chase M.W., Ronsted N., Devey D.S., PillonY., Pires J.C., Petersen G., Seberg O., Davis J.I. 2006. Phylogenetics of Liliales: summarized evidence from combined analyses of five plastid and one mitochondrial loci. Ranc. Sant. An. Bot. Gard. 22: 559-565.

Gabryszewska E., Hempel M. 1985. The influence of cytokinins and auxins on Alstroemeria in tissue culture. Acta Hort. 167: 295-300.

George E.F., Hall M.A., Klerk G.J.D. 2008. Plant Propagation by Tissue Culture. 3rd Edition, Springer, pp. 175-204. DOI 10.1007/978-1-4020-5005-3.

Gless C., Lörz H., Jähne-Gärtner A. 1998. Establishment of a highly efficient regeneration system from leaf base segments of oat (Avena sativa L.). Plant Cell Rep. 17: 441-445.

Hakkaart F.A., Versluijs J.M.A. 1988. Virus elimination by meristem tip culture from arrange of Alstroemeria cultivars. Neth. Plant Pathol. 94: 49-56.

Hansena G., Wright M.S. 1999. Recent advances in the transformation of plants. Trends Plant Sci. 4: 226-231. DOI 10.1016/S1360-1385(99)01412-0.

Hoshino Y., Murata N., Shinoda K. 2006. Isolation of individual egg cells and zygotes in Alstroemeria followed by manual selection with a microcapillary- connected micropump. Ann Bot. 97: 1139-1144. DOI 10.1093/aob/mcl072.

Hutchinson M.J., Onamu R., Kipkosgei L., Obukosia S.D. 2010. Effect of thidiazuron, NAA and BAP on in vitro propagation of Alstroemeria aurantiaca cv. ‘Rosita’ from shoot tip explants. J. Agri. Sci.Tech. 12: 60-69.

Kalimuthu K., Paulsamy S., Senthilkumar R., Sathya M. 2007. In vitro propagation of the biodiesel plant Jatropha curcas L. Plant Tiss. Cult. Biotechnol. 17: 137-147.

Kim J.B., Raemakers C.J.J.M., Jacobsen E., Visser R.G.F. 2006. Efficient somatic embryogenesis from leaves with axil tissue in Alstroemeria. Plant Cell Tiss. Org. Cult. 86: 233-238. DOI: 10.1007/s11240-006-9110-6.

Kristiansen K., Ørnstrup H., Brandt B. 1999. In vitro PPFD and media composition affect both in and ex vitro performance of Alstroemeria Butterflyhybrids. Plant Cell Tiss. Org. Cult. 56: 145-153.

Lee J.H., Han T.H. 2006. Identification of parental species of the Alstroemeria cv. ‘Jubilee’ using AFLP marker technique. Sci. Hort. 111: 63-67. DOI: 10.1016/j.scienta.2006.08.001.

Lin W.C., Monette P.L. 1987. In vitro propagation of Alstroemeria “Alsaan”. Plant Cell Tiss. Org. Cult. 9: 29-35.

Lin H.S., De Jeu M.J., Jacobsen E. 1997. Direct shoot regeneration from excised leaf explant of in vitro grown seedlings of Alstroemeria L. Plant Cell Rep. 16: 770-774. DOI: 10.1007/s002990050317.

Lin H.S., De Jeu M.J., Jacobsen E. 1998. Formation of shoots from leaf axils of Alstroemeria: the effect of position on the stem. Plant Cell Tiss. Org. Cult. 52: 165-169.

Lin H.S., De Jeu M.J., Jacobsen E. 2000a. Development of a plant regeneration system based on friable embryogenic callus in the ornamental Alstroemeria.Plant Cell Rep. 19: 529-534. DOI: 10.1007/s002990050768.

Lin H.S., De Jeu M.J., Jacobsen E. 2000b. The application of leafy explant micropropagation protocol in enhancing the multiplication efficiency of Alstroemeria.Sci. Hort. 85: 307-318. DOI: 10.1016/S0304-4238(00)00125-4.

Monette P.L. 1992. Micropropagation of Inca Lily (Alstroemeria spp.). In: Bajaj Y.P.S. (Ed.), Biotechnology in Agriculture and Forestry, Vol. 20, High- Tech and Micropropagation IV, pp. 1-18.

Murashige T., Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-496.

Pedersen C., Brandt K. 1992. A method for disinfection of underground rhizome tips of Alstroemeria and Heliconia. Acta Hort. 325: 499-504.

Pedersen C., Hansen C.W., Brandt K., Kristiansen K.1996. Alstroemeria plantlets can be induced to flowering by cold treatment during in vitro culture.Sci. Hort. 66: 217-228. DOI: 10.1016/S0304-4238(96)00917-X.

Pedraza-Santos M.E., López-Peralta M.C., González V.A., Engleman-Clark E.M., Sánchez-García P.2006. In vitro regeneration of Alstroemeria cv.‘Yellow King’ by direct organogenesis. Plant Cell Tiss. Org. Cult. 184: 189-198. DOI: 10.1007/s11240-005-9020-z.

Pierik R.L.M., Van Voorst A., Booy G.,Van Acker C.A.M., Lelivelt C.L.C., De Wit J.C. 1988. Vegetative propagation of Alstroemeria hybrids in vitro. Acta Hort. 226: 81-89.

Podwyszynska M., Gabryszewska E., Przybyla A. 1998.Effect of growth retardants, cytokinins and auxins on the multiplication and rooting in vitro of Alstroemeria × hybrida ‘Juanita’. Acta Agron. 51: 23-31. DOI: 10.5586/aa.1998.003.

Robinson G.W. 1963. Alstroemeria. J. Roy. Hortic. Soc. 88: 490-494.

Rustanius P., Hang A., Hughes H.G., Tsuchiya T. 1991.Chromosome analysis of Alstroemeria ligtu hybrids.Hort Sci. 26 (7): 902-904.SAS Institute 2002. SAS/STAT User’s Guide Release 9.Cary, NC, USA.

Van Zaayen A. 1995. Alstroemeria. In: Loebenstein G., Lawson R.H., Brunt A.A. (Eds.) Virus and Viruslike Diseases of Bulbs and Flower Crops, Wiley Publishers, Chichester, UK, pp. 237-249.

Van Zaayen C., Van E., Versluijs J.M.A. 1992. Production of high quality, healthy ornamental crops through meristem culture. Acta Bot. Neerl. 41: 425-433.

Journal Information

CiteScore 2017: 0.53

SCImago Journal Rank (SJR) 2017: 0.196
Source Normalized Impact per Paper (SNIP) 2017: 0.494


All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 71 71 26
PDF Downloads 14 14 5