New Basal Media for Protocorm-Like Body and Callus Induction of Hybrid Cymbidium

High frequency protocorm-like body (PLB) production from hybrid Cymbidium Twilight Moon ‘Day Light’ has been developed through a new medium, Teixeira Cymbidium (TC) medium. Two new TC media containing variable amounts of macroand micronutrients and other additives, inspired by Winarto and Teixeira (WT) medium for Anthurium and Murashige and Skoog (MS) basal medium were used to induce PLBs and callus. Control medium was research- and industry-standard Vacin and Went (VW) medium. The first TC medium, TCPLB, could induce significantly more PLBs than on VW while high levels of macronutrients in the second TC medium, TCCALLUS, and MS were required to induce callus. All PLB induction media contained 0.1 mg/l α-naphthaleneacetic acid (NAA) and 0.1 mg/l kinetin (KIN), 2 g/l tryptone and 20 g/l sucrose, and solidified with 8 g/l Bacto agar while callusinduction media were identical, except that KIN was substituted by thidiazuron (TDZ). Basal medium had a significant effect on PLB and callus formation. This protocol could be used to induce PLBs and callus from other Cymbidium species or cultivars.