A Europe-Wide Experiment for Assessing the Impact of Genotype-Environment Interactions on the Vitality and Performance of Honey Bee Colonies: Experimental Design and Trait Evaluation

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A Europe-Wide Experiment for Assessing the Impact of Genotype-Environment Interactions on the Vitality and Performance of Honey Bee Colonies: Experimental Design and Trait Evaluation

An international experiment to estimate the importance of genotype-environment interactions on vitality and performance of honey bees and on colony losses was run between July 2009 and March 2012. Altogether 621 bee colonies, involving 16 different genetic origins of European honey bees, were tested in 21 locations spread in 11 countries. The genetic strains belonged to the subspecies A. m. carnica, A. m. ligustica, A. m. macedonica, A. m. mellifera, A. m. siciliana. At each location, the local strain of bees was tested together with at least two "foreign" origins, with a minimum starting number of 10 colonies per origin. The common test protocol for all the colonies took into account colony survival, bee population in spring, summer and autumn, honey production, pollen collection, swarming, gentleness, hygienic behaviour, Varroa destructor infestation, Nosema spp. infection and viruses. Data collection was performed according to uniform methods. No chemical treatments against Varroa or other diseases were applied during the experiment. This article describes the details of the experiment set-up and the work protocol.

Aizen M. A., Harder L. D. (2009) - The global stock of domesticated honey bees is growing slower than agricultural demand for pollination. Current Biology, 19: 915-918.

Büchler R., Berg S., Kezic, N., Pechhacker, H., Van Praagh, J., Bubalo D., Ritter W., Bienefeld K. (2002) - Survival test without treatment against varroatosis - the island project in Croatia. Apidologie, 33: 493-494.

Büchler R., Garrido C., Bienefeld K., Ehrhardt K. (2007) - German honey bee selection program on disease tolerance. Proceedings 40th International Apicultural Congress, Melbourne, Australia, 399.

Büchler R., Garrido C., Bienefeld K., Ehrhardt K. (2008) - Selection for Varroa tolerance: concept and results of a long-term selection project. Apidologie, 39(5): 598.

Büchler R., Berg S., Le Conte Y. (2010) - Breeding for resistance to Varroa destructor in Europe. Apidologie, 41(3): 393-408.

Chauzat M. P., Carpentier P., Martel A. C., Bougeard S., Cougoule N., Porta P., Lachaize J., Madec F., Aubert M., Faucon J. P. (2009) - Influence of pesticide residues on honey bee (Hymenoptera: Apidae) colony health in France. Environ. Entomol. 38: 514-523.

Crane E. (1990) - Bees and Beekeeping. Science, Practice and World resources. Cornell University Press, Ithaca, New York.

Cox-Foster D. L., Conlan S., Holmes E. C., Palacios G., Evans J. D., Moran N. A., Quan P. L., Briese T., Hornig M., Geiser D. M., Martinson V., vanEngelsdorp D., Kalkstein A. L., Drysdale A., Hui J., Zhai J., Cui L., Hutchinson S. K., Simons J.F., Egolm M., Pettis J. S., Lipkin W. I. (2007) - A metagenomic survey of microbes in honey bee colony collapse disorder. Science, 318: 283-287.

De Miranda J. R., Genersch E. (2010) - Deformed Wing Virus. J. Invertebr. Pathol., 103 Suppl.1: 48-61.

De Miranda J. R., Cordoni G., Budge G. (2010) - The Acute-Bee-Paralysis Kashmir-Bee-Virus Israeli - Acute - Paralysis - Virus complex. J. Invertebr. Pathol., 103 Suppl. 1: 30-47.

Desneux N., Decourtye A., Delpuech J. M. (2007) - The sublethal effects of pesticides on beneficial arthropods. Annu. Rev. Entomol., 52, 81-106.

Fries I., Bommarco R. (2007) - Possible hostparasite adaptations in honey bees infested by Varroa destructor mites. Apidologie, 38(6): 525-533.

Gallai N., Michel Salles J. M., Settele J., Vaissière B. E. (2009) - Economic valuation of the vulnerability of world agriculture confronted with pollinator decline. Ecol. Econom., 68(3): 810-821. DOI: 10.1016/j.ecolecon.2008.06.014

Garrido C., Büchler R., Bienefeld K., Ehrhardt K. (2005) - Breeding for tolerance against Varroosis factors influencing colony survival without treatment in a long-term survey. Proceedings of 39th Apimondia congress, Dublin, 21.-26.08.2005, 76.

Harris J. W. (2007) - Bees with Varroasensitive hygiene preferentially remove miteinfested pupae aged ≤ 5 days postcapping. J. Apic. Res., 46(3): 134-139.

Harris J. W., Harbo J. R. (2000) - Changes in reproduction of Varroa destructor after honey bee queens were exchanged between resistant and susceptible colonies. Apidologie, 31: 689-699. DOI.org/10.1051/apido:2000153

Higes M., Martin R., Meana A. (2006) - Nosema ceranae, a new microsporidian parasite in honeybees in Europe. J. Invertebr. Pathol., 92: 93-95.

Ibrahim A., Spivak M. (2006) - The relationship between hygienic behaviour and suppression of mite reproduction as honey bee (Apis mellifera) mechanisms of resistance to Varroa destructor. Apidologie, 37(1): 31-40. DOI.org/10.1051/apido:2005052

Imdorf A., Buehlmann G., Gerig L., Kilchenmann V., Wille H. (1987) - Überprüfung der Schätzmethode zur Ermittlung der Brutfläche und der Anzahl Arbeiterinnen in freifliegenden Bienenvölkern. Apidologie, 18(2): 137-146.

Johnson R. M., Evans J. D., Robinson G. E., Berenboum M. R. (2009) - Changes in transcript abundance relating to colony collapse disorder in honey bees (Apis mellifera). Proceedings of the National Academy of Sciences, 106(35): 14790-14795.

Koeniger N., Schmidt J., Wilde J., Kefuss J., Ducos de Lahitte J. (1995) - Versuche zur Varroatosetoleranz von Bienen aus Uruquay in Europa. Pszczeln. Zesz. Nauk., 39(1): 121-131.

Le Conte Y., De Vaublanc G., Crauser D., Jeanne F., Rousselle J. C., Bécard J. M. (2007) - Honey bee colonies that have survived Varroa destructor. Apidologie, 38: 566-572.

Martìn-Hernández R., Meana A., Prieto L., Martìnez Salvador A., Garrido-Bailón E., Higes M. (2007) - Outcome of colonization of Apis mellifera by Nosema ceranae. Appl. Environ. Microb. 73: 6331-6338.

Meixner M. D., Costa C., Kryger P., Hatjina F., Bouga M., Ivanova E., Büchler R., (2010) - Conserving diversity and vitality for honey bee breeding. J. Apic. Res., 49(1): 85-92.

Neumann P., Carreck N. L. (2010) - Honey bee colony losses. J. Apic. Res., 49(1): 1-6. DOI: 10.3896/IBRA.1.49.1.01

Nguyen B. K., Saegerman C., Pirard C., Mignon J., Widart J., Thirionet B., Verheggen F. J., Berkvens D., De Pauw E., Haubruge E. (2009) - Does imidacloprid seed-treated maize have an impact on honey bee mortality? J. Econ. Entomol., 102: 616-623.

Potts S. G., Biesmeijer J. C., Kremen C., Neumann P., Schweiger O., Kunin W. E. (2010) - Global pollinator declines: trends, impacts and drivers. Trends in Ecology & Evolution, 25: 345-353.

Rinderer T. E., De Guzman L. I., Delatte G. T., Stelzer J. A., Lancaster V. A., Kuznetsov V., Beaman L., Watts R., Harris J. W. (2001) - Resistance to the parasitic mite Varroa destructor in honey bees from far eastern Russia. Apidologie, 31: 381-394.

Rinderer T. E., De Guzman L. I., Sylvester H. A. (2004) - Reexamination of the accuracy of a detergent solution for varroa mite detection. Am. Bee J., 144: 560-562.

Rosenkranz P., Aumeier P., Ziegelmann B. (2009) - Biology and control of Varroa destructor. J. Invertebr. Pathol., 103, 96-S119.

Ruttner H. (1972) - Technical recommendations for methods of evaluating performance of bee colonies. In F. Ruttner, Controlled mating and selection of the honey bee Bucharest, Apimondia, pp. 87-92.

Seeley T. D. (2007) - Honey bees of the Arnot forest: a population of feral colonies persisting with Varroa destructor in the northeastern Unites States. Apidologie, 38:19-29.

Siuda M., Wilde J., Koeniger N. (1996) - Further Research on Honeybee Breeding with Short Post-capping Periods. Pszczeln. Zesz. Nauk., 40(2): 135-143.

Spivak M. (1996) - Honey bee hygienic behaviour and defence against Varroa jacobsoni. Apidologie, 27: 245-260.

Spivak M., Reuter G. S. (2001) - Varroa destructor infestation in untreated honey bee (Hymenoptera: Apidae) colonies selected for hygienic behaviour. J. Econ. Entomol., 94: 326-331.

vanEngelsdorp D., Meixner M. D. (2010) - A historical review of managed honey bee populations in Europe and the United States and the factors that may affect them. J. Invertebr. Pathol., 103, 80-95.

Wilde J. (1994) - Hodowla pszczół o skróconym okresie postlarwalnym, odpornych na Varroa jacobsoni Oudemans. [Breeding honey bees for short post-capping period resistant to Varroa jacobsoni Oudemans]. Acta Academiae Agriculturae ac Technicae Olstenensis, Zootechnica, Suplementum B 39: 1-43.

Wilde J., Koeniger N. (1992a) - Breeding for a short post-capping period in Apis mellifera carnica worker brood after initial crossing with Apis mellifera capensis. Apidologie, 23(4): 429-431.

Wilde J., Koeniger N. (1992b) - Selektion auf Verkürzung der Zellverdeckelungsdauer (ZVD) der Arbeiterinnenbrut von Apis mellifera carnica. Annales Universitatis Mariae Curie-Skłodowska, Sectio DD, 47(25): 133-136.

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