Exposure-dependent variation in cryolite induced lethality in the nontarget insect, Drosophila melanogaster

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Abstract

The starting point of toxicity testing of any chemical in an organism is the determination of its Lethal Concentration 50 (LC50). In the present study, LC50 of a fluorinated insecticide cryolite is determined in a non-target insect model, Drosophila melanogaster. Interestingly, the result shows that acute LC50 of cryolite was much greater in comparison to the chronic one in case of Drosophila larvae. Larvae which were exposed to 65,000 to 70,000 μg/ml cryolite through food showed 50% mortality after 18 hours of acute exposure, whereas only 150 to 160 μg/ml cryolite was sufficient to cause 50% mortality in case of chronic exposure. Thus cryolite in a small amount when applied once cannot produce noticeable changes in Drosophila, whereas the same amount when used continuously can be fatal. The non-feeding pupal stage was also seen to be affected by chemical treatment. This suggests that the test chemical affects the developmental fate and results in failure of adult emergence. Absence of chemical-induced mortality in adults assumes that the toxicity of cryolite might be restricted to the preimaginal stages of the organism. Reduction in body size of larvae after ingestion of cryolite (with food) in acute treatment schedule is another interesting finding of this study. Some individuals consuming cryolite containing food cannot survive whereas the few survivors manifest a significant growth retardation which might be due to a tendency of refusal in feeding. Hence the present findings provide a scope of assessment of risk of other similar non-target groups

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  • Chen Y. (2003). Differences in fluoride effects on fecundity among varieties of the silkworm Bombyx mori. Fluoride 36(3): 163-169.

  • Dad I Yousuf MJ and Anjum SI. (2011). Determination of LC50 of Chlorpyrifos and Neem extract on third instar larvae of house flies and their effect on fecundity. J Basic App Sci 7(2): 169-174.

  • Das SK Podder S Roy S. (2010). Effect of fungicide Thiovit®Jet on several life history trait of Drosophila melanogaster (Diptera: Drosophilidae). JABS 4(3): 31-36.

  • Delong DM. (1934). The present status of cryolite as an insectide. Ohio J Sci 34(3): 175-200.

  • Edgar BA Orr-Weaver TL. (2001). Endoreplication cell cycles: more for less. Cell 105: 297-306.

  • Ellisor LO Floyd EH. (1939). Further investigations on the control of the velvetbean caterpillar Anticarsia gemmenatilis (Hbm.). J Econ Ent Menasha Wis 32(6): 863-867.

  • Fluoride Detective. (2012). Available on: http://fluoridedetective.com/fluoride-facts/cryolite (Accessed: 30 April 2012).

  • Gupta SC Siddique HR Saxena DK Kar Chowdhuri D. (2005). Comparative toxic potential of market formulation of two organophosphate pesticides in transgenic Drosophila melanogaster (hsp70-lacZ). Cell Biol Toxicol 21: 149-162.

  • Jahan M Ahmed I Naqvi SNH. (1990). Toxicteratogenic effects of Juliflurine and Morgosan-OTM on the Musca domestica L. larvae. Proc Pak Cong Zool 10: 293-299.

  • Jatav KV Agrawala D Kumar S Sharma S. (2011). Lethal efficiency of Acorus calamus in larval and adult stages of Drosophila melanogaster. WJST 1(10): 64-73.

  • Karatas A Bahceci Z. (2009). Toxic effects of Diazinon on adult individuals of Drosophila melanogaster. JABS. 3 (2): 102-108.

  • Khan MW Naqvi SNH Ahmed I Tabassum R Muhammad FA. (1991). Toxicology of crude neem extracts (N-4 & N-9) against late 2nd instar larvae of Musca domestica L. (PCSIR strain). Pak J Pharm Sci 4: 77-82.

  • Largent EJ. (1948). The comparative toxicity of Cryolite for rats and rabbits. J Industry Hyg 30: 92-97.

  • Lawrenz M Mitchell HH Ruth WA. (1939). The comparative toxicity of fluorine in calcium fluoride and in cryolite. J Nutrition 18(2): 115-125.

  • Majumdar TN Gupta A. (2012). Acute and chronic toxicity of copper on aquatic insect Chironomus ramosus from Assam India. J Environ Biol 33(1): 139-142.

  • Mitchell B Gerdes RA. (1973). Mutagenic effect of sodium and stannous fluoride upon Drosophila melanogaster. Fluoride 6(2): 113-117.

  • Nazir A Mukhopadhyay I Saxena DK Kar Choudhuri D. (2001). Chlorpyrifos- induced hsp70 expression and effect on reproductive performance in transgenic Drosophila melanogaster (hsp70-lacZ) Bg9. Arch. Environ Contam Toxicol 41(4): 443-449.

  • Nazir A Saxena DK Kar Choudhuri D. (2003). Induction of hsp70 in transgenic Drosophila: biomarker of exposure against phthalimide group of chemicals. Biochem Biophys Acta 1621(2): 218-225.

  • Paumen ML Borgman E Kraak MHS van Gestel CAM Admiraal W. (2008). Life cycle responses of the midge Chironomus riparius to polycyclic aromatic compound exposure. Environ Pollut 108: 225-232.

  • Podder S Roy S. (2013). Study of the changes in life cycle parameters of Drosophila melanogaster exposed to fluorinated insecticide Cryolite. Toxicol Ind Health. DOI: 10.1177/0748233713493823.

  • Reddy KL Rovani MK Wohlwill A Katzen A Storti RV. (2006). The Drosophila Par domain protein I gene Pdp1 is a regulator of larval growth mitosis and endoreplication. Dev Biol. 289(1): 100-114.

  • Ware GW Whitacre DM. (2004). An introduction to insecticides in The Pesticide Book - 6th ed. (Ware GW ed) Meister Publishing Co. Willoughby Ohio.

  • Wene G Hansberry R. (1944). Toxicity of cryolite to Mexican bean beetle larvae. J Econ Ent 37(5): 656-659.

  • United States Environmental Protection Agency (US-EPA). (1996). Reregistration eligibility decision - Cryolite Case 0087 EPA-738-R-96-016.

  • United States Environmental Protection Agency (US-EPA). (1997). The cryolite task force; Pesticide Tolerance petition filing [PF-712; FRL-5587-7]. Federal Register 62(48): 42546-42551

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