Protective efficacy of 2-PAMCl, atropine and curcumin against dichlorvos induced toxicity in rats

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Protective efficacy of 2-PAMCl, atropine and curcumin against dichlorvos induced toxicity in rats

The effect of 2- pyridine aldoxime methyl chloride (2-PAMCl) and atropine with or without curcumin was investigated in dichlorvos (2,2-dichlorovinyl dimethyl phosphate; DDVP) induced toxicity in rats. Rats were exposed to DDVP (2 mg/kg sub-cutaneously) once daily for the period of 21 days. Post DDVP exposure, rats were further treated with 2-PAMCl (50 mg/kg intramuscular, once daily) + atropine (10 mg/kg, i.m. once daily) with or without curcumin (200 mg/kg; oral; once daily) for further 21 days. We observed a significant increase in lipid peroxidation (LPO), reactive oxygen species (ROS), oxidized glutathione (GSSG), while there was a significant decrease in antioxidant enzymes, brain acetylcholinesterase (AChE) and 5-hydroxy tryptamine (5-HT) activity on DDVP exposure of rats. These alterations were restored significantly by co-administration of 2-PAMCl + atropine in DDVP exposed rats. Curcumin when co-supplemented with 2-PAMCl + atropine also significantly protected serum aspartate aminotransferase (AST) and restored brain AChE activity and 5-HT level in animals sub-chronically exposed to DDVP. Histopathological observations along with biochemical changes in rat blood and tissues revealed significant protection offered by 2-PAMCl + atropine against DDVP. The results indicate that DDVP-induced toxicity can be significantly protected by co-administration of 2-PAMCl + atropine individually, however, curcumin co-supplementation with 2-PAMCl + atropine provides more pronounced protection, concerning particularly neurological disorders.

Abdollahi M, Rainba A, Shadnia S, Nikfar S, Rezaie A. (2004). Pesticide and oxidative stress: a review. Med Sci Monitor 10: RA141-RA147.

Aebi H. (1984). Catalase, in Methods in Enzymol, vol. 105, (Packer L, ed) pp. 125-126, Academic Press, Orlando, FL.

Anand P, Thomas SG, Kunnumakkara AB, Sundaram C, Harik-umar KB, Sung B, Tharakan ST, Misra K, Priyadarsini IK, Rajasekharan KN, Aggarwal BB. (2008). Biological activities of curcumin and its analogues (Congeners) made by man and Mother Nature. Biochem Pharmacol 76: 1590-1611.

Ataman Kose, Nurullah Gunay, Beril Kose, Ali R. Ocak, Ozcan Erel, Abdullah T. Demiryurek. (2010). Effects of atropine and pralidoxime pretreatment on serum and cardiac oxidative stress parameters in acute dichlorvos toxicity in rats. Pestic. Biochem Physiol. 97: 249-255.

Bengmark S. (2006). Curcumin, an atoxic antioxidant and natural NFkappaB, cyclooxygenase-2, lipooxygenase, and inducible nitric oxide synthase inhibitor: a shield against acute and chronic diseases. JPEN J Parenter Enteral Nutr 30: 45-51.

Celik I, Isik I. (2009). Neurotoxic effects of subacute exposure to dichlorvos and methyl parathion at sublethal dosages in rats. Pestic. Biochem Physiol 94: 1-4.

Celik I, Suzek H. (2008). Effects of subacute exposure to dichlorvos at sublethal dosages on erythrocyte and tissue antioxidant defense systems and lipid peroxidation in rats, Fd. Chem. Toxicol. 46: 2796-2801.

Delgado Eduardo HB, Streck EL, Quevedo JL, Dal-Pizzol F. (2006). Mitochondrial respiratory dysfunction and oxidative stress after chronic malathion exposure. Neurochemistry Research 31: 1021-1025.

Dwivedi N, Bhutia YD, Kumar V, Yadav P, Kushwaha P, Swarnkar H, Flora SJS. (2010). Effects of combined exposure to dichlorvos and monocrotophos on blood and brain biochemical variables in rats. Human Exp. Toxicol. 29: 121-129.

Dwivedi N, Flora SJS. (2011). Concomitant exposure to arsenic and organophosphates on tissue oxidative stress in rats. Fd Chem Toxicol. 49: 1152-1159.

Ellman GL. (1959). Tissue sulphydryl groups. Arch Biochem 82: 70-77.

Ellman GL, Courtney KD, Andres V, Feaderstone RM. (1961). A new and rapid colorimetric determination of acetyl cholinesterase activity. Biochem Pharmacol 7: 88-95.

Fu Y, Zheng S, Lin J, Ryerse J, Chen A. (2008). Curcumin protects the rat liver from CCl4-caused injury and fibrogenesis by attenuating oxidative stress and suppressing inflammation. Mol. Pharmacol. 73: 399-409.

Hissin PJ, Hilf R. (1976). A fluorometric method for the determination of oxidized and reduced glutathione in tissues. Anal Biochem 74: 214-216.

Hodgson E. (2004). A Textbook of Modern Toxicology, 3rd ed. John Wiley and Sons, Inc., New Jersey, pp. 203-211.

Jacobwitz DW, Richardson JS. (1978). Method for rapid determination of norepinephrine, dopamine and serotonin in the same brain region. Pharmacol Biochem Behav 8: 515-519.

Jollow DJ, Mitchell JR, Zamppaglione Z, Gillette JR. (1974). Bromobenzene induced liver necrosis. Protective role of glutathione and evidence for 3, 4-bromobenzene oxide as the hepatotoxic metabolite. Pharmacology 11: 151.

Kakkar P, Das B, Viswanathan PN. (1984). A modified spectrophotometric assay of superoxide dismutase. Ind J Biochem Biophy 21: 130-132.

Kassa J. (2005). The role of oximes in the antidotal treatment of chemical casualities exposed to nerve agents, in Medical Aspects of Chemical and Biological Terrorism; Chemical Terrorism and Traumatism, (Monov A, Dishovsky C, eds) pp. 193-208, Publishing House of the Union of Scientists in Bulgaria, Sofia, Bulgaria.

Llopis SP, Ferrando MD, Pena JB. (2003). Fish tolerance to organophosphate induced oxidative stress is dependent on the glutathione metabolism and enhanced by N-acetylcysteine. Aquat. Toxicol. 65: 337-360.

Maheshwari RK, Singh AK, Gaddipati J, Srimal RC. (2006). Multiple biological activities of curcumin: a short review. Life Sci 78: 2081-2087.

Manal, E. A., Elhalwagy Nevine, S., Darwish, Enass, M., Zaher, 2008. Prophylactic effect of green tea polyphenols against liver and kidney injury induced by fenitrothion insecticide. Pest. Biochem. Physiol. 91(2): 81-89.

McEvoy G. (2002). Anticholinergic agents. AHFS drug information 2002, American Hospital Formulary Service, Bethesda, MD. pp 1222-1228.

Miquel J, Bernd A, Sempere JM, Diaz-Alperi J, Ramirez A. (2002). The curcuma antioxidants: pharmacological effects and prospects for future clinical use. A review. Arch Geronto Geriatr 34: 37.

Mittal M, Flora SJS. (2006). Effects of individual and combined exposure to sodium arsenite and sodium fluoride on tissue oxidative stress, arsenic and fluoride levels in male mice. Chem. Biol. Inter. 162: 128-139.

Ohkawa H, Ohishi N, Yagi K. (1979). Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95: 351-358.

Ojha A, Yaduvanshi SK, Srivastava N. (2011). Effect of combined exposure of commonly used organophosphate pesticides on lipid peroxidation and antioxidant enzymes in rat tissues. Pesticide Biochem Physiol. 99: 148-156.

Reddy AC, Lokesh BR. (1994). Effect of dietary turmeric. (Curcuma longa) on iron-induced lipid peroxidation in the rat liver. Fd Chem Toxicol. 32: 279-283.

Reitman S, Frankel S. (1957). A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am J Clin Pathol 28: 56-63.

Savolainen K. (2001). Understanding the toxic action of organophosphates, in Handbook of Pesticide Toxicology, vol. 2., (Krieger RI, ed) pp 1013-1043, Academic Press, USA.

Shenouda J, et al. (2009). An evaluation of the inhibition of human butyrylcholinesterase and acetylcholinesterase by the organophosphate chlorpyrifos oxon. Toxicol Appl Pharmacol 241: 135-142.

Slotkin TA. (2004). Cholinergic systems in brain development and disruption by neurotoxicants: nicotine, environmental tobacco smoke, organophosphates. Toxicol Appl Pharmacol 198: 132-151.

Slotkin TA. (2005). Developmental neurotoxicity of organophosphates: a case study of chlorpyrifos, in Toxicity of Organophosphate and Carbamate Pesticides, (Gupta RC, ed) pp. 293-314, Elsevier Academic Press, San Diego.

Socci DJ, Bjugstad KB, Jones HC, Pattisapu JV, Arendash GW. (1999). Evidence that oxidative stress is associated with the pathophysiology of inherited hydrocephalus in the H-Tx rat model. Exp Neurol 155: 109-17.

Soobrattee MA, Neergheen VS, Luximon-Ramma A, Aruoma OI, Bahorun T. (2005). Phenolic as potential antioxidant theraputic agents: Mechanism and actions. Mut Res 579: 200-213.

Stojiljkovic MP, Jokanovic M. (2005). AUM shinrikyo and terrorist use of nerve agents in Japan, in Medical Aspects of Chemical and Biological Terrorism; Chemical Terrorism and Traumatism, (Monov A, Dishovsky C, eds) pp. 101-115, Publishing House of the Union of Scientists in Bulgaria, Sofia, Bulgaria.

Taylor P. (2001). Anticholinesterase agents, in The Pharmacological Basis of Therapeutics, 10th edition (Hardman GJ, Limbird LE, Gilman AG. eds) pp. 110-129, McGraw-Hill, New York.

Tirkey N, Kaur G, Vij G, Chopra K. (2005). Curcumin, a diferuloylmethane, attenuates cyclosporine-induced renal dysfunction and oxidative stress in rat kidneys. BMC Pharmacol. 5: 15.

Weissman BA, Raveh L. (2008). Therapy against organophosphate poisoning: The importance of anticholinergic drugs with antiglutamatergic properties. Toxicol Appl Pharmacol 232: 351-358.

Zhu QH, Huang JX, Lin Z. (2005). Therapeutic efficacy of pralidoxime chloride on acute dichlorvos poisoning. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi 23: 91-93.

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The Journal of Institute of Experimental Pharmacology of Slovak Academy of Sciences

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