There are no reports of addition of carob fibre to gluten-free bread, as only carob germ flour was used. The research task was to determine what level of carob fibre can be used and how it influences the physical and sensorial properties of gluten-free bread. Especially, the knowledge of the antioxidant properties of such bread is very valuable. The gluten-free bread from rice, corn, and buckwheat flour (35:35:30%) was prepared after mixing (5 min), proofing (40 min, 30°C), and baking (45-50 min, 230°C) of dough. Carob fibre was added in the amounts of 1, 2, 3, 4, and 5% of the total flour content. The results showed that increased content of carob fibre induced significant and favourable changes in the volume, colour, and texture (hardness and springiness) of the bread crumb. Carob fibre enriched the breads with lipophilic compounds able to chelate metal ions. The activity of hydrophilic compounds was significantly higher in the case of control bread and bread with the lowest percentage of the additive. In conclusion, the highest increase in antioxidant activity was found for breads with 1 and 2% of carob fibre. The most acceptable gluten-free bread can be obtained by adding up to 2% of carob.
Ayaz F.A., Torun H., Ayaz S., Correia P.J., Alaiz M., Sanz C., Gruz J., and Strand M., 2007. Determination of chemical composition of Anatolian carob pod (Ceratonia siliqua L.): Sugars, amino and organic acids, minerals and phenolic compounds. J. Food Quality, 30, 1040-1055.
Gawlik-Dziki U., Świeca M., Dziki D., Baraniak B., Tomiło J., and Czyż J., 2013. Quality and antioxidant properties of breads enriched with dry onion (Allium cepa L.) skin. Food Chemistry, 138, 1621-1628.
Gámbaro A., Gimenez A., Ares G., and Gilardi V., 2006. Influence of enzymes on the texture of brown pan bread. J. Texture Studies, 37, 300-314.
Guo J.T., Lee H.L., Chiang S.H., Lin H.I., and Chang C.Y., 2001. Antioxidant properties of the extracts from different parts of broccoli in Taiwan. J. Food Drug Analysis, 9, 96-101.
Haber B., 2002. Carob fiber benefits and applications. Cereal Food Word, 4, 41-47.
Hager A.S., Wolter A., Czerny M., Bez J., Zannini E., Arendt E.K., and Czerny M., 2012. Investigation of product quality, sensory profile and ultrastructure of breads made from a range of commercial gluten-free flours compared to their wheat counterparts. European Food Res. Technol., 235, 333-344.
ISO 20483:2006. Cereals and pulses – determination of the nitrogen content and calculation of the crude protein content-Kjeldahl method).
ISO 2171:2007. Cereals, pulses and by-products – determination of ash yield by incineration. The International Organization for Standardization.
ISO 11085:2008. Cereals, cereals-based products and animal feeding stuffs – determination of crude fat and total fat content by the randall extraction method.
Klenow S., Glei M., Haber B., Owen R., and Pool-Zobel B.L., 2008. Carob fiber compounds modulate parameters of cell growth differently in human HT29 colon adenocarcinoma cells than in LT97 colon adenoma cells. Food Chemical Toxicology, 46, 1389-1397.
Lim H.S., Park S.H., Ghafoor K., Hwang S.Y., and Park J., 2011. Quality and antioxidant property of bread containing turmeric (Curcuma longa L.) cultivated in South Korea. Food Chemistry, 112, 1577-1582.
Martínez Mario M., Díaz A., and Gómez M., 2014. Effect of different microstructural features of soluble and insoluble fibres on gluten-free dough rheology and bread-making. J. Food Eng., 142, 49-56.
Matos M.E. and Rosell C.M., 2012. Relationship between instrumental parameters and sensory characteristics in gluten-free breads. European Food Research Technol., 235(1), 107-117.
Miś A., Grundas S., Dziki D., and Laskowski J., 2012. Use of farinograph measurements for predicting extensograph traits of bread dough enriched with carob fibre and oat wholemeal. J. Food Eng., 108(1), 1-12.
Naghmouchi S., Khouja M.L., Romero A., Tous J., and Boussaid M., 2009. Tunisian carob (Ceratonia siliqua L.) populations: Morphological variability of pods and kernel. Sci. Hortic. – Amsterdam, 121, 125-130.
Oyaizu M., 1986. Studies on products of browning reaction – Antioxidative activities of products of browning reaction prepared from glucosamine. Japanese J. Nutrition, 44, 307-315.
Papagiannopoulos M., Wollseifen H.R., Mellenthin A., Haber B., and Galensa R., 2004. Identification and quantification of polyphenols in carob fruits (Ceratonia siliqua L.) and derived products by HPLC-UV-ESI/MSn. J. Agric. Food Chemistry, 52, 3784-3791.
Re R., Pellegrini N., Proteggente A., Pannala A., Yang M., and Rice-Evans C., 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology Medicine, 6, 1231-1237.
Różyło R., 2014. New potential in using millet-based yeast fermented leaven for composite wheat bread preparation. J. Food Nutrition Res., 53(3), 240-250.
Różyło R., Dziki D., and Laskowski J., 2014a. Changes in the physical and the sensorial properties of wheat bread caused by interruption and slowing of the fermentation of yeast-based leaven. J. Cereal Sci., 59, 88-94.
Różyło R., Dziki D., Laskowski J., Skonecki S., Łysiak G., Kulig R., and Różyło K., 2014b. Texture and sensory evaluation of composite wheat-oat bread prepared with novel two-phase method using oat yeast fermented leaven. J. Texture Studies, 45, 235-245.
Różyło R., Dziki D., Gawlik-Dziki U., Cacak-Pietrzak G., Miś A., and Rudy S., 2015a. Physical properties of gluten-free bread caused by water addition. Int. Agrophys., 29, 353-364.
Różyło R., Rudy S., Krzykowski A., and Dziki D., 2015b. Novel application of freeze-dried amaranth sourdough in gluten-free bread production. J. Food Process Eng., 38, 135-143.
Różyło R., Rudy S., Krzykowski A., Dziki D., Gawlik-Dziki U., Różyło K., and Skonecki S., 2015 c. Effect of adding fresh and freeze-dried buckwheat sourdough on gluten-free bread quality. Int. J. Food Sci. Technol., 50, 313-322.
Sakac M., Torbica A., Sedej I., and Hadnadev M., 2011. Influence of breadmaking on antioxidant capacity of gluten free breads based on rice and buckwheat flours. Food Res. Int., 44(9), 2806-2813.
Salinas M.V., Carbas B., Brites C., and Puppo M.C., 2015. Influence of different carob fruit flours (Ceratonia siliqua L.) on wheat dough performance and bread quality. Food Bioprocess Technol., 8, 1561-1570.
Siastała M., Dziki D., and Różyło R., 2014. The influence of carob pod fiber on textural properties of wheat bread crumb (in Polish). Zeszyty Problemowe Postępów Nauk Rolniczych, 578, 111-119.
Singleton V.L. and Rossi J., 1965. Colorimetry of total phenolics with phosphomolybdic-phosphotungstics acid reagents. American J. Enology Viticulture, 16, 144-158.
Smith B.M., Bean S.R., Herald T.J., and Aramouni F.M., 2012. Effect of HPMC on the quality of wheat-free bread made from carob germ flour-starch mixtures. J. Food Sci., 77(6), C 684-C 689.
Su X.Y., Wanga Z.Y., and Liu J.R., 2009. In vitro and in vivo antioxidant activity of Pinus koraiensis seed extract containing phenolic compound. Food Chem., 117, 681-686.
Tsatsaragkou K., Gounaropoulos G., and Mandala I., 2014a. Development of gluten free bread containing carob flour and resistant starch. LWT – Food Science and Technology, 58, 124-129.
Tsatsaragkou K., Yiannopoulos S., Kontogiorgi A., Poulli E., Krokida M., and Mandala I., 2014b. Effect of carob flour addition on the rheological properties of gluten-free breads. Food Bioprocess Technol., 7, 868-876.
Tsatsaragkou K., Yiannopoulos S., Kontogiorgi A., Poulli E., Krokida M., and Mandala I., 2012. Mathematical approach of structural and textural properties of gluten free bread enriched with carob flour. J. Cereal Science, 56, 603-609.
Wang J., Rosell C.M., and Benedito de Barber C., 2002. Effect of the addition of different fibers on wheat dough performance and bread quality. Food Chemistry, 79, 221-226.
Wronkowska M. and Soral-Śmietana M., 2008. Buckwheat flour – a valuable component of gluten-free formulations. Polish J. Food Nutrition Sci., 581, 59-63.
Zunft H.J., Luder W., Harde A., Haber B., Graubaum H.J., Koebnick C., and Grunwald J., 2003. Carob pulp preparation rich in insoluble fibre lowers total and LDL cholesterol in hypercholesterolemic patients. European J. Nutrition, 42, 235-242.