Effect of temperature on oxidative stress induced by lead in the leaves of Plantago major L.

Open access


Fluctuation of the summer day-time temperatures in the mid-latitudes in a range from 16 to 30°C should not have irreversible negative effects on plants, but may influence metabolic processes including the oxidative stress. To test the effect of moderately high temperature on oxidative stress induced by lead in the leaves of Plantago major L.; the plants were incubated in a water solution of 0, 150, 450, and 900 μM Pb (NO3)2 at 20 and 28°C. Plant reactions were evaluated by the content of thiobarbituric acid reactive substances and ascorbate peroxidase and glutathione reductase activities in leaves after 2, 24, 48, and 72 h. The Pb concentration in the leaves rose with the increase in the Pb content and was higher at 20°C. The increase in stomatal resistance caused by Pb was higher at 28°C. The contents of TBARS increased after 2 h of plant exposure to Pb and the increase was the highest at 900 μM Pb, 28°C. The AsP activity increased up to 50% after 24 h of Pb-treatment at 28°C; the highest increase in glutathione reductase activity was observed after 72 h at 20°C. Thus, the moderately high temperature 28°C compared with optimal 20°C caused a decrease in Pb accumulation in Plantago leaves but amplified the negative effects of lead, especially in the beginning of stress development.

Ahmad M.S.A., Hussain M., Ijaz S., and Alvi A.K., 2008. Photosynthetic performance of two mung bean Vigna radiata cultivars under lead and copper stress. Int. J. Agr. Biol., 10, 167-172.

Atkin O.K., Scheurwater I., and Pons T.L., 2007. Respiration as a percentage of daily photosynthesis in whole plants is homeostatic at moderate, but not high, growth temperatures. New Phytol., 174, 367-380.

Bafeel S.O., 2010. Physiological and biochemical aspects of tolerance in Lepidium sativum cress to lead toxicity. Catrina, 51, 1-7.

Balakhnina T.I., Gavrilov A.B., Wlodarczyk T.M., Borkowska A., Nosalewicz M., and Fomina I.R., 2009. Dihydroquercetin protects barley seeds against mould and increases seedling adaptive potential under soil flooding. Plant Growth Regul., 57, 127-135.

Balakhnina T.I., Kosobryukhov A.A., Ivanov A.A., and Kreslavskii V.D., 2005. The effect of cadmium on CO2 exchange, variable fluorescence of chlorophyll, and the level of antioxidant enzymes in pea leaves. Russ. J. Plant Physiol., 521, 15-20.

Balakhnina T.I., Bennicelli R., Stępniewska Z., Stępniewski W., Borkowska A., and Fomina I.R., 2012a. Stress responses of spring rape plants to soil flooding. Int Agrophys., 26, 347-353.

Balakhnina T.I., Matichenkov V.V., Wlodarczyk T., Borkowska A., Nosalewicz M., and Fomina I.R., 2012b. Effects of silicon on growth processes and adaptive potential of barley plants under optimal soil watering and flooding. Plant Growth Regul., 67, 35-43.

Balakhnina T.I. and Borkowska A., 2013. Effects of silicon on plant resistance to environmental stresses: review. Int. Agrophys., 27, 225-232.

Bazzaz F.A., Rolfe G.L., and Windle P., 1974. Differing sensitivity of corn and soybean photosynthesis and transpiration to lead contamination. J. Environ. Qual., 3, 156-158.

Chaitanya K.V., Sundar D., Masilamani S. and Ramachandra Reddy A., 2002. Variation in heat stress-induced antioxidant enzyme activities among three mulberry cultivars. Plant Growth Regul., 36, 175-180.

Chaitanya K.V., Sundar D., and Ramachandra Reddy A., 2001. Mulberry leaf metabolism under high temperature stress. Biol. Plant, 44, 379-384.

Choudhury S. and Panda S.K., 2005. Toxic effects, oxidative stress and ultrastructural changes in moss Taxithelium nepalense Schwaegr. Broth under chromium and lead phytotoxicity. Water Air Soil Poll., 167, 73-90.

Erdei S., Hegedus A., Hauptmann G., Szalai J., and Horvath G., 2002. Heavy metal induced physiological changes in the antioxidative response system. Acta Biol. Szegediensis, 46, 89-90.

Filipović-Trajković R., Ilić Z.S., Šunić L., and Andjelković S., 2012. The potential of different plant species for heavy metals accumulation and distribution. J. Food Agric. Environ., 10, 959-964.

Foyer C.H. and Halliwell B., 1976. The presence of glutathione and glutathione reductase in chloroplasts: a proposed role in ascorbic acid metabolism. Planta, 133, 21-25.

Gajic G., Mitrovic M., Pavlovic P., Stevanovic B., Djurdjevic L., and Kostic O., 2009. An assessment of the tolerance of Ligustrum ovalifolium Hassk to traffic-generated Pb using physiological and biochemical markers. Ecotox. Environ. Saf., 72, 1090-1101.

Geebelen W., Vangronsveld J., Adriano D.C., Poucke L.C., and Van Clijsters H., 2002. Effects of Pb-EDTA and EDTA on oxidative stress reactions and mineral uptake in Phaseolus vulgaris. Physiol. Plant, 115, 377-384.

Girard S., Clement A., Cochard H., Boulet-Gercourt B., Guehl J-M., and Anderson J.A., 2002. Catalase activity, hydrogen peroxide content and thermotolerance of pepper leaves. Sci. Hort., 95, 277-284.

Gulen H. and Eris A., 2004. Effect of heat stress on peroxidase activity and total protein content in strawberry plants. Plant Sci., 166, 739-744.

Gupta D.K., Nicoloso F.T., Schetinger M.R.C., Rossato L.V., Pereira L.B., Castro G.Y., Srivastava S., and Tripathi R.D., 2009. Antioxidant defense mechanism in hydroponically grown Zea mays seedlings under moderate lead stress. J. Hazard. Mater., 172,479-484.

Halliwell B. and Gutteridge J.M.C., 1999. Free radicals in biology and medicined. Oxford University Press, New York, USA.

Hoagland D.R. and Arnon D.I., 1938. The water-culture method for growing plants without soil. University of California, College of Agriculture, Agricultural Experiment Station, Berkeley.

Islam E., Liu D., Li T.Q., Yang X., Jin X.F., Mahmooda Q., Tian S., and Li J.Y., 2008. Effect of Pb Toxicity on leaf growth, physiology and ultrastructure in the two ecotypes of Elsholtzia argyi. J. Hazard. Mater., 154, 914-926.

Kabata-Pendias A. and Pendias H., 1992. Trace elements in soils and plants. CRC Press, Boca Ratón, FL, USA.

Kosobryukhov A., Knyazeva I., and Mudrik V., 2004. Plantago major plants responses to increase content of lead in soil: Growth and photosynthesis. Plant Growth Regul., 42, 145-151.

Li X., Bu N., Li Y., Ma L., Xin Sh., and Zhang L., 2012. Growth, photosynthesis and antioxidant responses of endophyte infected and non-infected rice under lead stress conditions. J. Hazard. Mater., 213-214, 55-61.

Lichtenthaller H.K. and Wellburn A.K., 1983. Determination of total carotenoids and chlorophyll ‘a’ and ‘b’ of leaf extracts in different solvents. Biochem. Soc. Trans., 11, 591-592.

Lipiec J., Doussan C., Nosalewicz A., and Kondracka K., 2013. Effect of drought and heat stresses on plant growth and yield: a review. Int Agrophys., 27, 463-477.

Liu D., Zou J., Meng Q., Zou J., and Jiang W., 2009. Uptake and accumulation and oxidative stress in garlic Allium sativum L. under lead phytotoxicity. Ecotoxicol., 18, 134-43.

Malar S., Sahi S.V., Favas P.J.C., and Venkatachalam P., 2014. Lead heavy metal toxicity induced changes on growth and antioxidative enzymes level in water hyacinths Eichhornia crassipes (Mart.). Bot. Stud., 55, http://www.as-botanical-studies.com/content/55/1/54

Malecka A., Jarmuszkiewicz W., and Tomaszewska B., 2001. Antioxidative defense to lead stress in subcellular compartments of pea roots. Acta Biochim. Polon., 48, 687-698.

Mishra S., Srivastava S., Tripathi R.D., Kumar R., Seth C.S., and Gupta D.K., 2006. Lead detoxification by coontail Ceratophyllum demersum L. involves induction of phytochelatins and antioxidant system in response to its accumulation. Chemosphere, 65, 1027-1039.

Mudrik V., Kosobrukhov A., Knyazeva I., and Pigulevskaya T., 2003. Changes in the photosynthetic characteristics of Plantago major plants caused by soil drought stress. Plant Growth Regul., 40, 1-6.

Nakano Y. and Asada K., 1981. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol., 22, 867-880.

Nosalewicz M., Stępniewska Z., and Nosalewicz A., 2013. Effect of soil moisture and temperature on N2O and CO2 concentrations in soil irrigated with purified wastewater. Int Agrophys., 27, 299-304.

Reddy A.M., Kumar S.G., Jyonthsnakumari G., Thimmanaik S., and Sudhakar C., 2005. Lead induced changes in antioxidant metabolism of horsegram Macrotyloma uniflorum Lam. Verdc. and bengalgram Cicer arietinum L. Chemosphere, 60, 97-104.

Ruley A.T., Sharma N.C., and Sahi S.V., 2004. Antioxidant defense in a lead accumulating plant, Sesbania drummondii. Plant Physiol. Biochem., 42, 899-906.

Sękara A., Poniedziałek M., Ciura J., and Jędrszczyk E., 2005. Cadmium and lead accumulation and distribution in the organs of nine crops: Implications for phytoremediation. Polish J. Environ. Stud., 14(4), 509-516.

Sharma P. and Dubey R.S., 2005. Lead toxicity in plants. Braz. J. Plant Physiol., 17, 35-52.

Shen Z.G. and Liu Y.L., 1998. Progress in the study on the plants that hyperaccumulate heavy metal. Plant Physiol. Commun., 34, 133-139.

Suzuki N., Koussevitzky S., Mittler R., and Miller G., 2012. ROS and redox signaling in the response of plants to abiotic stress. Plant, Cell Environ., 35, 59-270.

Takeda R., Yukiko S., Rumi Y., Sadao K., and Akiyoshi S., 2006. Accumulation of heavy metals by cucumber and Brassica juncea under different cultivation conditions. Proc. Annual Int. Conf. Soils, Sediments, Water and Energy, http://scholarworks.umass.edu/soilsproceedings/vol11/iss1/19

Uchiyama M. and Mihara M., 1978. Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Anal. Biochem., 86, 287-297.

Uysal Y. and Taner F., 2011. The evaluation of the PbII removal efficiency of duckweed Lemna minor L. from aquatic mediums at different conditions In: Survival and sustainability. Environmental earth sciences (Eds H. Gökçekus, U. Türker, J.W. LaMoreaux). Springer-Verlag, Berlin-Heidelberg.

Verma S. and Dubey R.S., 2003 Lead toxicity induces lipid peroxidation and alters the activities of antioxidant enzymes in growing rice plants. Plant Sci., 164, 645-655.

Voskresenskaya O.L., Voskresenskiy V.S., and Alyabysheva E.A., 2013. Accumulation of heavy metals in soil and plants in location gathering and temporary storage solid waste (in Russian). Modern Problems of Science and Education, Biological Sciences, 2: http://www.science-education.ru/108-r8659

Wahid A., Gelani S., Ashraf M., and Foolad M.R., 2007. Heat tolerance in plants: An overview. Environ. Exp. Bot., 61, 199-223.

Wang H., Shan X., Wen B., Owen G., Fang J., and Zhang S., 2007. Effect of indole-3-acetic acid on lead accumulation in maize Zea mays L. seedlings and the relevant antioxidant response. Environ. Exp. Bot., 61, 246-253.

Xiong Z-T., 1997. Bioaccumulation and physiological effects of excess lead in a roadside pioneer species Sonchus oleraceus L. Environ. Pollut., 973, 275-279.

Yoon J., Cao X., Zhou Q., and Ma L.Q., 2006. Accumulation of Pb, Cu, and Zn in native plants growing on a contaminated Florida site. Sci. Total Environ., 368, 456-464.

International Agrophysics

The Journal of Institute of Agrophysics of Polish Academy of Sciences

Journal Information

IMPACT FACTOR 2017: 1.242
5-year IMPACT FACTOR: 1.267

CiteScore 2017: 1.38

SCImago Journal Rank (SJR) 2017: 0.435
Source Normalized Impact per Paper (SNIP) 2017: 0.849


All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 191 191 8
PDF Downloads 62 62 5