Pot experiments were designed to test the applicability of root electrical capacitance measurement for in situ monitoring of root water uptake activity by growing cucumber and bean cultivars in a growth chamber. Half of the plants were inoculated with Funneliformis mosseae arbuscular mycorrhizal fungi, while the other half served as non-infected controls. Root electrical capacitance and daily transpiration were monitored during the whole plant ontogeny. Phenology-dependent changes of daily transpiration (related to root water uptake) and root electrical capacitance proved to be similar as they showed upward trends from seedling emergence to the beginning of flowering stage, and thereafter decreased continuously during fruit setting. A few days after arbuscular mycorrhizal fungi-colonization, daily transpiration and root electrical capacitance of infected plants became significantly higher than those of non-infected counterparts, and the relative increment of the measured parameters was greater for the more highly mycorrhizal-dependent bean cultivar compared to that of cucumber. Arbuscular mycorrhizal fungi colonization caused 29 and 69% relative increment in shoot dry mass for cucumbers and beans, respectively. Mycorrhization resulted in 37% increase in root dry mass for beans, but no significant difference was observed for cucumbers. Results indicate the potential of root electrical capacitance measurements for monitoring the changes and differences of root water uptake rate.
Aston M.J. and Lawlor D.W., 1979. The relationship between transpiration, root water uptake and leaf water potential. J.Exp. Botany, 114, 169-181.
Aubrecht L., Staněk Z., and Koller J., 2006. Electrical measurement of the absorption surfaces of tree roots by the earth impedance methods: 1. Theory. Tree Physiol., 26, 1105-1112.
Augé R.M., 2001. Water relations, drought and vesicular-arbuscular mycorrhizal symbiosis. Mycorrhiza, 11, 3-42.
Cao Y., Repo T., Silvennionen R., Lehto T., and Pelkonen P., 2010. An appraisal of the electrical resistance method for assessing root surface area. J. Exp. Botany, 61, 2491-2497.
Čermák J., Ulrich R., Staněk Z., Koller J., and Aubrecht L., 2006. Electrical measurement of tree root absorbing surfaces by the earth impedance method: 2. Verification based on allometric relationships and root severing experiments. Tree Physiol., 26, 1113-1121.
Chloupek O., 1972. The relationship between electric capacitance and some other parameters of plant roots. Biologia Plantarum, 14, 227-230.
Chloupek O., Dostál V., Středa T., Psota V., and Dvořáčková O., 2010. Drought tolerance of barley varieties in relation to their root system size. Plant Breeding, 129, 630-636.
Cseresnyés I., Fekete G., Végh R.K., Székács A., Mörtl M., and Rajkai K., 2012. Monitoring of herbicide effect in maize based on electrical measurements. Int. Agrophys., 26, 243-247.
Cseresnyés I., Rajkai K., and Vozáry E., 2013a. Role of phase angle measurement in electrical impedance spectroscopy. Int. Agrophys., 27, 377-383.
Cseresnyés I., Takács T., Végh R.K., Anton A., and Rajkai K., 2013b. Electrical impedance and capacitance method: a new approach for detection of functional aspects of arbuscular mycorrhizal colonization in maize. Eur. J. Soil Biol., 54, 25-31.
Ellis T., Murray W., and Kavalieris L., 2013. Electrical capacitance of bean (Vicia faba) root systems was related to tissue density - a test for the Dalton Model. Plant Soil, 366, 575-584.
Fu S., Cheng W., and Susfalk R., 2002. Rhizosphere respiration varies with plant species and phenology: a greenhouse pot experiment. Plant Soil, 239, 133-140.
Gao S., Pan W.L., and Koenig R.T., 1998. Integrated root system age in relation to plant nutrient uptake activity. Agronomy J., 90, 505-510.
Lai C-T. and Katul G., 2000. The dynamic role of root water-uptake in coupling potential to actual transpiration. Advances Water Res., 23, 427-439.
Medrano E., Lorenzo P., Sánchez-Guerrero M.C., and Montero J.I., 2005. Evaluation and modelling of greenhouse cucumber crop transpiration under high and low radiation conditions. Scientia Horticulturae, 105, 163-175.
Muleta D., 2010. Legume responses to arbuscular mycorrhizal fungi inoculation in sustainable agriculture. In: Inoculation Microbes for Legume Improvement (Eds M.S Khan, A. Zaidi, J. Musarrat). Springer Press, Wien, Austria.
Nomiyama R., Yasutake D., Sago Y., and Kitano M., 2013. Transpiration integrated model for root ion absorption under salinized condition. Biologia, 68, 1113-1117.
Novák V. and Vidovič J., 2003. Transpiration and nutrient uptake dynamics in maize (Zea mays L.). Ecol. Model., 166, 99-107.
Ozier-Lafontaine H. and Bajazet T., 2005. Analysis of root growth by impedance spectroscopy (EIS). Plant Soil, 277, 299-313.
Phillips J.M. and Hayman D.S., 1970. Improved procedures for clearing roots and staining parasitic and vesiculararbuscular mycorrhizal fungi for rapid assessment of infection. Trans. British Mycological Soc., 55, 157-160.
Rajkai K., Végh R.K., and Nacsa T., 2005. Electrical capacitance of roots in relation to plant electrodes, measuring frequency and root media. Acta Agron. Hung., 53, 197-210.
Siddique K.H.M., Belford R.K., and Tennant D., 1990. Root:shoot ratios of old and modern, tall and semi-dwarf wheats in a Mediterranean environment. Plant Soil, 121, 89-98.
Svačina P., Středa T., and Chloupek O., 2014. Uncommon selection by root system size increases barley yield. Agron. Sustain. Dev., 34, 545-551.
Yang X., Short T.H., Fox R.D., and Bauerle W.L., 1990. Transpiration, leaf temperature and stomatal resistance of a greenhouse cucumber crop. Agric. Forest Meteorol., 51, 197-209